Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   V3829_RS18495 Genome accession   NZ_CP144358
Coordinates   3736604..3737041 (+) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain DJ4     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3731604..3742041
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V3829_RS18480 - 3734462..3734824 (-) 363 WP_014304320.1 winged helix-turn-helix transcriptional regulator -
  V3829_RS18485 hxlA 3735056..3735691 (+) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  V3829_RS18490 hxlB 3735688..3736245 (+) 558 WP_014304318.1 6-phospho-3-hexuloisomerase -
  V3829_RS18495 nucA/comI 3736604..3737041 (+) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  V3829_RS18500 nin/comJ 3737063..3737461 (+) 399 WP_043866822.1 competence protein ComJ Regulator
  V3829_RS18505 - 3737502..3737720 (-) 219 Protein_3589 family 1 glycosylhydrolase -
  V3829_RS20260 - 3737752..3737997 (-) 246 WP_109567708.1 family 1 glycosylhydrolase -
  V3829_RS18510 - 3737939..3738214 (-) 276 WP_369719109.1 YckD family protein -
  V3829_RS18515 - 3738331..3738765 (-) 435 WP_021494203.1 RDD family protein -
  V3829_RS18520 - 3738780..3739916 (-) 1137 WP_048368274.1 zinc-dependent alcohol dehydrogenase -
  V3829_RS18525 - 3739981..3741183 (-) 1203 WP_331828023.1 GTP-binding protein -
  V3829_RS18530 - 3741336..3741461 (+) 126 Protein_3595 hypothetical protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=933758 V3829_RS18495 WP_003156588.1 3736604..3737041(+) (nucA/comI) [Bacillus velezensis strain DJ4]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=933758 V3829_RS18495 WP_003156588.1 3736604..3737041(+) (nucA/comI) [Bacillus velezensis strain DJ4]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCAATAAAAGAAGGGCATTCCAGCATTTGCACCATTGATAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641