Detailed information NTG0933724

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: V3829_RS07260
• Genome accession: NZ_CP144358
• Coordinates: 1425170..1426315 (+)
• Length: 381 a.a.
• NCBI ID: WP_003151067.1
• Uniprot ID: A0A6A8LBV3
• Organism: Bacillus velezensis strain DJ4
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 1420170..1431315

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V3829_RS07240	-	1420644..1421360 (-)	717	WP_014306017.1	Crp/Fnr family transcriptional regulator	-
V3829_RS07245	narK	1421458..1422648 (-)	1191	WP_095315495.1	nitrate transporter NarK	-
V3829_RS07250	argS	1422783..1424453 (-)	1671	WP_003151071.1	arginine--tRNA ligase	-
V3829_RS07255	-	1424450..1424878 (-)	429	WP_003151069.1	DUF1934 domain-containing protein	-
V3829_RS07260	rapF	1425170..1426315 (+)	1146	WP_003151067.1	tetratricopeptide repeat protein	Regulator
V3829_RS07265	-	1426299..1426418 (+)	120	WP_003151066.1	PhrC/PhrF family phosphatase-inhibitory pheromone	-
V3829_RS07270	-	1426617..1426820 (+)	204	WP_003151064.1	helix-turn-helix transcriptional regulator	-
V3829_RS07275	-	1426834..1427037 (+)	204	WP_003151062.1	hypothetical protein	-
V3829_RS07285	-	1427445..1427726 (+)	282	WP_003151060.1	hypothetical protein	-
V3829_RS07290	-	1427770..1428282 (+)	513	WP_194468841.1	stage II sporulation protein M	-
V3829_RS07295	-	1428873..1429091 (-)	219	WP_003151056.1	helix-turn-helix transcriptional regulator	-
V3829_RS07300	speB	1429261..1430133 (-)	873	WP_003151054.1	agmatinase	-
V3829_RS07305	speE	1430193..1431023 (-)	831	WP_003151052.1	spermidine synthase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45339.49 Da
• Isoelectric Point: 4.9293

>NTDB_id=933724 V3829_RS07260 WP_003151067.1 1425170..1426315(+) (rapF) [Bacillus velezensis strain DJ4]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEEDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELDQREYLSAIKFFKKAETKLSFIKDTIDKAEYFFKMSEVYYYLKQTYVS
TDYARQAYEIYKAQSSYDIRLIQCQSLFGANFLDLKLYDRAITHFQKAFSLAETEKQPQLMGRTLYNIALCKQSLNDYEA
AITYVEKAIDVFESAKMTPSLPQAYFLMTQIHYKLERYDKALEYHKKGVIYSEESADSVYLHEFEFLKSLYVNGPDEDAM
KRSFDFLESKMMYADIEDFALDAAKYYHKLEDYKKASTYFLKVEEARQHIQGGVTFYEAEV

Nucleotide

• Length: 1146 bp

>NTDB_id=933724 V3829_RS07260 WP_003151067.1 1425170..1426315(+) (rapF) [Bacillus velezensis strain DJ4]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAGCTTGCAGGCCTGGAAGAAGATCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGTTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGATCAGCGGGAATATTTGTCGGCTATAAAATTTTTTAAAAAGGCTGAGACGAAACTCTCATTTA
TTAAGGATACTATTGATAAAGCCGAGTACTTTTTCAAGATGTCTGAAGTCTACTACTATTTGAAACAAACGTATGTATCT
ACCGATTACGCAAGGCAGGCATATGAAATATATAAAGCTCAGTCGTCATACGATATCAGGCTCATACAATGCCAATCCTT
ATTCGGAGCAAATTTCCTGGATTTAAAACTATATGATCGGGCAATTACTCACTTTCAAAAGGCTTTTTCTTTGGCAGAAA
CAGAAAAACAGCCTCAATTAATGGGCAGGACGCTGTATAACATCGCACTTTGCAAACAAAGCCTGAACGACTATGAAGCA
GCTATAACTTATGTTGAAAAAGCCATTGATGTTTTTGAAAGTGCTAAAATGACCCCTTCTTTGCCCCAAGCATATTTTTT
AATGACACAGATTCATTATAAATTAGAAAGATACGACAAAGCGCTCGAGTATCATAAAAAAGGAGTTATCTATTCCGAAG
AGTCAGCAGATTCAGTTTATTTACATGAATTCGAATTTTTAAAATCTTTGTATGTGAACGGACCTGATGAGGATGCAATG
AAACGGAGTTTTGACTTTTTGGAAAGTAAGATGATGTATGCAGATATAGAAGATTTTGCTCTTGATGCAGCTAAATATTA
TCACAAACTTGAAGATTATAAAAAAGCTTCAACATACTTTTTAAAAGTTGAAGAAGCAAGACAGCATATTCAAGGGGGGG
TAACTTTTTATGAAGCTGAAGTATAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (5-65)

• Tetratricopeptide repeat (TPR_12) (223-287)

3D structure

• AlphaFold DB: A0A6A8LBV3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	74.803	100	0.748
rapC	Bacillus subtilis subsp. subtilis str. 168	53.158	99.738	0.53