Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   caldi_RS15885 Genome accession   NZ_AP025628
Coordinates   3274627..3276042 (+) Length   471 a.a.
NCBI ID   WP_264842723.1    Uniprot ID   A0AA35CN01
Organism   Caldinitratiruptor microaerophilus strain JCM 16183     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3269627..3281042
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  caldi_RS15865 (caldi_32080) - 3269630..3270169 (+) 540 WP_264842719.1 UvrB/UvrC motif-containing protein -
  caldi_RS15870 (caldi_32090) - 3270156..3271226 (+) 1071 WP_264842720.1 protein arginine kinase -
  caldi_RS15875 (caldi_32100) - 3271313..3273778 (+) 2466 WP_264842721.1 ATP-dependent Clp protease ATP-binding subunit -
  caldi_RS15880 (caldi_32110) - 3273898..3274416 (-) 519 WP_264842722.1 DoxX family protein -
  caldi_RS15885 (caldi_32120) radA 3274627..3276042 (+) 1416 WP_264842723.1 DNA repair protein RadA Machinery gene
  caldi_RS15890 (caldi_32130) disA 3276036..3277097 (+) 1062 WP_264842724.1 DNA integrity scanning diadenylate cyclase DisA -
  caldi_RS15895 (caldi_32140) - 3277167..3277562 (-) 396 WP_264842725.1 DUF1573 domain-containing protein -
  caldi_RS15900 (caldi_32150) - 3278054..3279142 (+) 1089 WP_264842726.1 bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase type II -
  caldi_RS17840 - 3279189..3279287 (+) 99 Protein_3137 CarD family transcriptional regulator -
  caldi_RS15905 (caldi_32160) - 3279397..3280527 (+) 1131 WP_264842727.1 PIN/TRAM domain-containing protein -

Sequence

Protein


Download         Length: 471 a.a.        Molecular weight: 49152.32 Da        Isoelectric Point: 6.1948

>NTDB_id=93331 caldi_RS15885 WP_264842723.1 3274627..3276042(+) (radA) [Caldinitratiruptor microaerophilus strain JCM 16183]
MARERTVFVCQECGQEALRWQGRCPGCGAWGTMVEERVPAPSGRGAAGRARAGAGPGAVGHLPVPIAEVDPGEAPRVVTG
IGELDRVLGGGLVPGSMVLVGGDPGIGKSTLLLQAAHRLALQGVGVLYVSGEESARQVRLRADRLGALAPGLYVASETDM
DQIEENVRVLSPGVLVVDSIQTVFRPDLSSAPGSVTQVREAASDLLRLAKSTGVTVLVVGHVTKEGAIAGPRVLEHIVDT
VLYFEGERQGQLRVLRAVKNRFGSTGEIGLFEMRDGGLAEVPGASQVLLAERPAGAAGSVVVPCVEGTRPLLVEVQALVS
ATPYPNPRRTATGVDLTRVQLILAVLEKRVGLHLGGHDAYIKVAGGVRVDEPAVDLGLAVALASSFREVPADPHTVVLGE
VGLAGEVRAVSRLAERLYEAEKLGFRRAVIPAGNLRAEGRRGDIEWVGVRTVAEGLEAALGGSREEVGQGW

Nucleotide


Download         Length: 1416 bp        

>NTDB_id=93331 caldi_RS15885 WP_264842723.1 3274627..3276042(+) (radA) [Caldinitratiruptor microaerophilus strain JCM 16183]
ATGGCGAGGGAACGCACGGTCTTCGTGTGCCAGGAGTGCGGGCAGGAGGCCCTGCGCTGGCAGGGCCGCTGCCCGGGGTG
CGGGGCGTGGGGGACCATGGTCGAGGAGCGGGTGCCCGCCCCCTCCGGGCGCGGTGCCGCGGGCCGTGCCCGGGCCGGCG
CCGGCCCCGGGGCGGTGGGGCACCTTCCCGTCCCCATCGCCGAGGTGGACCCCGGGGAGGCGCCCCGGGTGGTCACGGGG
ATCGGCGAACTGGACCGGGTCCTGGGCGGCGGGCTGGTCCCCGGGTCGATGGTCCTCGTGGGCGGCGACCCGGGCATCGG
CAAGTCGACTCTGCTCCTCCAGGCCGCCCACCGGCTGGCCCTGCAGGGCGTGGGGGTGCTGTACGTCTCCGGGGAGGAAT
CCGCCCGGCAGGTGCGCCTGCGGGCGGACCGCCTCGGGGCCCTCGCCCCGGGGCTCTACGTGGCCTCCGAGACCGACATG
GACCAGATCGAGGAGAACGTCCGGGTCCTGTCCCCGGGCGTCCTGGTCGTGGACTCCATCCAGACGGTGTTCCGCCCGGA
CCTCTCCAGCGCCCCGGGCAGCGTCACCCAGGTGCGTGAGGCGGCGTCCGATCTTCTGCGCCTCGCCAAGTCGACCGGGG
TCACGGTGCTGGTGGTGGGCCACGTCACCAAGGAGGGCGCCATCGCCGGGCCCCGGGTGCTCGAGCACATCGTCGACACG
GTCCTCTACTTCGAGGGCGAGCGCCAGGGCCAGCTGCGGGTGCTGCGGGCGGTAAAGAACCGGTTCGGGTCGACCGGGGA
GATCGGGCTCTTCGAGATGCGGGACGGCGGCCTCGCCGAGGTGCCCGGCGCTTCCCAGGTGCTCCTGGCCGAGCGCCCCG
CCGGCGCGGCCGGGTCCGTGGTGGTGCCGTGCGTGGAGGGCACCCGCCCCCTCCTCGTCGAGGTGCAGGCCCTGGTCAGC
GCCACCCCCTACCCGAACCCGCGCCGGACCGCCACCGGGGTCGACCTGACCCGGGTCCAGCTCATCCTGGCCGTGCTCGA
GAAGCGCGTGGGGCTCCACCTGGGCGGGCACGACGCCTACATCAAGGTGGCGGGCGGGGTGCGGGTCGACGAGCCGGCGG
TCGACCTCGGCCTGGCCGTGGCGCTCGCCTCCAGCTTCCGGGAGGTGCCGGCCGACCCCCATACGGTCGTGCTCGGCGAG
GTGGGCCTGGCCGGCGAGGTCCGGGCGGTGAGCCGGCTCGCCGAGCGTCTCTACGAGGCAGAGAAGCTGGGCTTCCGCCG
GGCGGTGATCCCGGCCGGCAACCTGCGGGCCGAGGGCCGGCGCGGGGACATCGAGTGGGTCGGCGTGCGGACGGTGGCGG
AGGGCCTGGAGGCCGCTCTGGGCGGGTCCCGTGAGGAGGTCGGGCAAGGGTGGTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(367-434)

AAA domain (AAA_25)

(87-226)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

56.587

98.301

0.556
  radA Streptococcus pneumoniae Rx1

50.435

97.665

0.493
  radA Streptococcus pneumoniae D39

50.435

97.665

0.493
  radA Streptococcus pneumoniae R6

50.435

97.665

0.493
  radA Streptococcus pneumoniae TIGR4

50.435

97.665

0.493
  radA Streptococcus mitis NCTC 12261

50.435

97.665

0.493
  radA Streptococcus mitis SK321

50.435

97.665

0.493

Multiple sequence alignment