Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   V1L70_RS19930 Genome accession   NZ_CP144278
Coordinates   4198105..4199538 (+) Length   477 a.a.
NCBI ID   WP_014171817.1    Uniprot ID   A0A839BTX8
Organism   Enterobacter ludwigii strain T977     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4193105..4204538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1L70_RS19900 (V1L70_19900) - 4193672..4194649 (+) 978 WP_020883528.1 calcium/sodium antiporter -
  V1L70_RS19905 (V1L70_19905) kdsD 4194663..4195649 (+) 987 WP_020883527.1 arabinose-5-phosphate isomerase KdsD -
  V1L70_RS19910 (V1L70_19910) kdsC 4195664..4196230 (+) 567 WP_014171813.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  V1L70_RS19915 (V1L70_19915) lptC 4196227..4196802 (+) 576 WP_014171814.1 LPS export ABC transporter periplasmic protein LptC -
  V1L70_RS19920 (V1L70_19920) lptA 4196771..4197325 (+) 555 WP_020883526.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  V1L70_RS19925 (V1L70_19925) lptB 4197332..4198057 (+) 726 WP_014171816.1 LPS export ABC transporter ATP-binding protein -
  V1L70_RS19930 (V1L70_19930) rpoN/rpoN1 4198105..4199538 (+) 1434 WP_014171817.1 RNA polymerase factor sigma-54 Machinery gene
  V1L70_RS19935 (V1L70_19935) hpf 4199561..4199848 (+) 288 WP_014171818.1 ribosome hibernation promoting factor -
  V1L70_RS19940 (V1L70_19940) ptsN 4199931..4200422 (+) 492 WP_014171819.1 PTS IIA-like nitrogen regulatory protein PtsN -
  V1L70_RS19945 (V1L70_19945) rapZ 4200468..4201322 (+) 855 WP_014171820.1 RNase adapter RapZ -
  V1L70_RS19950 (V1L70_19950) npr 4201319..4201591 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  V1L70_RS19955 (V1L70_19955) mtgA 4201630..4202355 (-) 726 WP_025202922.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  V1L70_RS19960 (V1L70_19960) elbB 4202352..4203005 (-) 654 WP_025202921.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54206.00 Da        Isoelectric Point: 4.3224

>NTDB_id=933266 V1L70_RS19930 WP_014171817.1 4198105..4199538(+) (rpoN/rpoN1) [Enterobacter ludwigii strain T977]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQADLHEEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESMGDEEIELEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSVRNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTILSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=933266 V1L70_RS19930 WP_014171817.1 4198105..4199538(+) (rpoN/rpoN1) [Enterobacter ludwigii strain T977]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCGATTCGCCTGTTGCA
ACTGTCCACCTTAGAACTCCAGCAGGAGCTCCAGCAAGCGCTGGATAGCAATCCGTTGCTGGAGCAAGCCGATCTTCATG
AAGAGGTAGATACCCAGCAGTCGCAGGATACTGAAGCACTCGACACCGCCGATGCGCTCGAACAAAAAGAGATGCCGGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGACGA
TGAGCTACCGGTTTATCAGGGTGAAACTACCCAGTCACTGCAGGATTACCTGATGTGGCAGGTGGAACTTACCCCCTTCT
CCGATACCGACCGCGCGATAGCGACATCCATTGTCGATGCCGTGGATGACACCGGTTATCTGACCGTCACGCTGGACGAA
ATTCTGGAAAGCATGGGTGACGAAGAGATTGAACTCGAGGAGATAGAAGCCGTTCTGAAGCGCATTCAGCGTTTCGATCC
GGTAGGGGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTTATTCAGCTTTCTCAGTTCAGCAAAGAGACGCCATGGCTTG
ATGAAGCCCGCTTGATCATCAGCGACCATCTGGATCTTCTGGCCAATCACGATTTCCGCAGCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAAGAGGCGGTCAATCTGATTCAGTCCCTCGATCCGCGCCCCGGTCAGTCCATTCAGACCAG
CGAACCCGAGTACGTCATCCCGGATGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAATTCGGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGTGCGTAACGACTCCGATAATCAATACATTCGT
AGCAATTTGCAGGAAGCGCGATGGTTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTCCGCGTAAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCCATTCGCGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTGAGCGACAGTAAGTTAACCACCATACTGTCCGACCAGGGTATTATGGTGG
CACGCCGAACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A839BTX8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.941

100

0.486