Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MWM02_RS00540 Genome accession   NZ_AP025627
Coordinates   103144..104517 (+) Length   457 a.a.
NCBI ID   WP_244402732.1    Uniprot ID   -
Organism   Parageobacillus sp. KH3-4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 98144..109517
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWM02_RS00520 (PspKH34_00780) - 98504..98965 (+) 462 WP_064550572.1 CtsR family transcriptional regulator -
  MWM02_RS00525 (PspKH34_00790) - 98980..99528 (+) 549 WP_064550571.1 UvrB/UvrC motif-containing protein -
  MWM02_RS00530 (PspKH34_00800) - 99533..100615 (+) 1083 WP_064550570.1 protein arginine kinase -
  MWM02_RS00535 (PspKH34_00810) clpC 100622..103057 (+) 2436 WP_064550569.1 ATP-dependent protease ATP-binding subunit ClpC -
  MWM02_RS00540 (PspKH34_00820) radA 103144..104517 (+) 1374 WP_244402732.1 DNA repair protein RadA Machinery gene
  MWM02_RS00545 (PspKH34_00830) - 104626..105720 (+) 1095 WP_064550567.1 PIN/TRAM domain-containing protein -
  MWM02_RS00550 (PspKH34_00840) ispD 105744..106430 (+) 687 WP_064550566.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MWM02_RS00555 (PspKH34_00850) ispF 106448..106924 (+) 477 WP_064550565.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  MWM02_RS00560 (PspKH34_00860) gltX 107033..108493 (+) 1461 WP_244402733.1 glutamate--tRNA ligase -
  MWM02_RS00565 (PspKH34_00870) cysE 108835..109503 (+) 669 WP_064550563.1 serine O-acetyltransferase -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49759.39 Da        Isoelectric Point: 7.2490

>NTDB_id=93273 MWM02_RS00540 WP_244402732.1 103144..104517(+) (radA) [Parageobacillus sp. KH3-4]
MGKKKTKFVCQECGYESAKWLGRCPGCNTWNSMVEEIERTKPATRGVFVHSAPDIISKPVPITTVTTTQEPRIQTSISEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLAALQHKVLYISGEESVKQTKLRADRLRVAADQLYVLAETDLEYIV
EAIETIHPACVVIDSIQTIYRADIASAPGSVSQVRECTAELMKIAKTKGIAIFIVGHVTKEGAIAGPRILEHMVDTVLYF
EGERHHTYRILRAVKNRFGSTNEIGIFEMRESGLVEVANPSEVFLEERSRGAAGSTVVAAMEGTRPVLVEIQALVSPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFRDQPTNPHDVIIGEVGLT
GEVRRVSRIEQRVQEAVKLGFSRMIIPTNNLAGWNPPEHITVIGVSHVAEALEYTLQ

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=93273 MWM02_RS00540 WP_244402732.1 103144..104517(+) (radA) [Parageobacillus sp. KH3-4]
ATGGGGAAAAAGAAAACAAAGTTTGTTTGTCAAGAATGCGGCTATGAGTCAGCGAAGTGGCTTGGAAGATGCCCAGGATG
CAATACGTGGAATTCGATGGTTGAGGAAATCGAGCGGACCAAGCCGGCAACAAGAGGAGTGTTTGTTCATTCCGCGCCTG
ATATCATCTCTAAGCCAGTGCCGATTACTACAGTGACAACGACGCAAGAGCCGCGCATACAAACGAGCATTTCCGAGTTT
AACCGCGTATTGGGCGGAGGGGTTGTGAAAGGTTCGCTCGTTTTAATCGGCGGCGATCCGGGGATTGGAAAATCGACGCT
TCTATTGCAAGTTTCTGCGCAACTTGCCGCTTTGCAGCATAAAGTATTATATATATCCGGCGAGGAATCAGTAAAACAAA
CGAAATTGCGCGCTGACCGCCTTCGTGTTGCTGCTGACCAGCTGTATGTATTAGCAGAGACAGATTTGGAATATATTGTA
GAAGCAATAGAAACGATTCACCCTGCTTGTGTTGTGATCGATTCGATCCAAACGATTTACCGCGCGGATATTGCGTCTGC
GCCCGGAAGTGTATCACAAGTTCGCGAGTGCACGGCAGAGCTGATGAAAATCGCCAAAACAAAAGGAATCGCCATTTTTA
TTGTCGGGCATGTAACAAAAGAAGGAGCGATTGCCGGACCAAGAATTTTAGAGCATATGGTGGACACCGTTCTTTATTTT
GAAGGCGAGCGCCATCATACGTATCGTATTTTGCGGGCGGTGAAAAACCGTTTCGGCTCCACAAATGAAATTGGTATTTT
CGAAATGAGAGAATCGGGGCTTGTTGAGGTCGCAAATCCATCGGAGGTGTTTTTAGAGGAGCGCTCCCGCGGTGCAGCAG
GTTCAACCGTTGTCGCGGCAATGGAAGGGACCCGTCCTGTGTTAGTCGAAATTCAAGCGTTAGTGTCACCAACGAGCTTT
GGTAATCCGCGGCGGATGGCGACAGGAATTGATCATAACCGTGTTTCTTTATTGATGGCCGTATTGGAAAAAAGGGTCGG
TCTGCTTTTACAAAATCAGGATGCTTACTTGAAAGTGGCAGGGGGAGTAAAATTAGATGAACCGGCGATTGATTTAGCGA
TTGCTGTGAGCATTGCCTCGAGCTTTCGCGATCAGCCTACAAATCCGCATGACGTCATTATTGGCGAAGTTGGTTTGACA
GGAGAAGTACGCCGTGTTTCCCGTATTGAACAGCGGGTGCAGGAAGCTGTAAAATTAGGATTTTCCCGCATGATTATCCC
TACAAACAATTTAGCAGGATGGAACCCGCCGGAACATATAACCGTTATCGGCGTGTCCCATGTCGCCGAAGCGCTGGAGT
ATACGTTGCAGTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

AAA domain (AAA_25)

(68-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

76.805

100

0.768
  radA Streptococcus mitis NCTC 12261

64.238

99.125

0.637
  radA Streptococcus mitis SK321

64.238

99.125

0.637
  radA Streptococcus pneumoniae Rx1

64.018

99.125

0.635
  radA Streptococcus pneumoniae D39

64.018

99.125

0.635
  radA Streptococcus pneumoniae R6

64.018

99.125

0.635
  radA Streptococcus pneumoniae TIGR4

64.018

99.125

0.635

Multiple sequence alignment