Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   VXF94_RS10305 Genome accession   NZ_CP143956
Coordinates   2028671..2030050 (-) Length   459 a.a.
NCBI ID   WP_013350792.1    Uniprot ID   A0A9P1NFR7
Organism   Bacillus amyloliquefaciens strain Fad 108     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2023671..2035050
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VXF94_RS10285 (VXF94_10285) ispF 2025190..2025666 (-) 477 WP_013350796.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  VXF94_RS10290 (VXF94_10290) ispD 2025659..2026357 (-) 699 WP_038463178.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  VXF94_RS10295 (VXF94_10295) - 2026371..2027471 (-) 1101 WP_013350794.1 PIN/TRAM domain-containing protein -
  VXF94_RS10300 (VXF94_10300) disA 2027585..2028667 (-) 1083 WP_014469611.1 DNA integrity scanning diadenylate cyclase DisA -
  VXF94_RS10305 (VXF94_10305) radA 2028671..2030050 (-) 1380 WP_013350792.1 DNA repair protein RadA Machinery gene
  VXF94_RS10310 (VXF94_10310) clpC 2030144..2032579 (-) 2436 WP_013350791.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  VXF94_RS10315 (VXF94_10315) - 2032576..2033667 (-) 1092 WP_013350790.1 protein arginine kinase -
  VXF94_RS10320 (VXF94_10320) - 2033667..2034224 (-) 558 WP_013350789.1 UvrB/UvrC motif-containing protein -
  VXF94_RS10325 (VXF94_10325) ctsR 2034238..2034702 (-) 465 WP_003156396.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49522.91 Da        Isoelectric Point: 7.1316

>NTDB_id=932205 VXF94_RS10305 WP_013350792.1 2028671..2030050(-) (radA) [Bacillus amyloliquefaciens strain Fad 108]
MAKSKTKFICHSCGYESAKWMGKCPGCGAWNTMVEETIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVQTKLEEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLADTAGSVLYISGEESVKQTKLRADRLGINSQLLHVLSETDMEYIS
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGASGSSIVASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMVIPEANSDGWTIPKGIEVVGVANVAEALRTSLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=932205 VXF94_RS10305 WP_013350792.1 2028671..2030050(-) (radA) [Bacillus amyloliquefaciens strain Fad 108]
ATGGCTAAATCAAAAACAAAATTCATCTGCCACTCATGCGGTTATGAATCCGCAAAATGGATGGGAAAGTGCCCGGGCTG
CGGGGCTTGGAATACAATGGTGGAAGAAACCATAAAAAAAGCGCCGGCGAATCGGCGGGCGGCTTTCTCCCATTCCGTTC
AAACCGTCCAGAAACCATCACCCATTACATCAATTGAAACATCAGAAGAACCCCGCGTTCAAACCAAGCTCGAAGAATTC
AACAGAGTGCTTGGCGGCGGAGTTGTGAAGGGCTCGTTAGTATTAATCGGCGGCGATCCCGGCATCGGTAAATCAACGCT
CCTTCTTCAAGTATCCGCACAATTAGCAGACACGGCCGGCAGCGTGCTTTACATTTCTGGGGAAGAATCTGTGAAGCAAA
CGAAGCTGCGCGCCGACAGACTTGGAATCAACAGCCAATTGTTACATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAAACCCGCATTTGTTGTGGTAGACTCCATTCAGACCGTTTACCAAAGTGATATCACCTCAGC
TCCCGGCAGTGTATCACAAGTAAGAGAGTGTACGGCCGAATTGATGAAAATCGCGAAAACAAACGGCATACCTATTTTTA
TCGTCGGACACGTTACAAAAGAAGGATCGATCGCAGGACCGAGATTGCTTGAGCATATGGTCGATACCGTTCTATACTTT
GAAGGAGAGCGGCACCATACATTCCGTATTTTGCGGGCTGTAAAAAACCGTTTCGGCTCAACAAATGAAATGGGCATCTT
TGAAATGCGGGAAGAAGGGCTTACAGAAGTGCTGAACCCTTCTGAAATTTTCTTAGAAGAGCGTTCTGCCGGGGCTTCGG
GTTCGAGTATCGTTGCGTCAATGGAAGGCACGAGACCGATCTTAGTTGAAATCCAGGCGCTCATATCACCGACCAGCTTC
GGAAATCCGCGCCGCATGGCTACAGGAATTGACCACAACAGGGTGTCGTTAATCATGGCTGTGCTTGAGAAGCGGGTGGG
GCTGTTGCTTCAAAATCAAGATGCGTATTTAAAAGTCGCCGGCGGGGTCAAACTGGATGAACCAGCGATTGATTTGGCAG
TAGCCGTCAGCATTGCTTCAAGCTTCAGAGATACACCGCCAAATCCTGCAGATTGCTTTATCGGTGAAGTGGGTCTGACA
GGAGAAGTCCGCAGGGTGTCAAGAATCGAACAGCGCGTAAAAGAAGCGGCGAAGCTCGGCTTCAAGCGTATGGTCATACC
TGAGGCAAATTCAGACGGATGGACAATACCAAAAGGGATTGAGGTTGTCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(71-222)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

93.231

99.782

0.93
  radA Streptococcus pneumoniae Rx1

63.797

98.693

0.63
  radA Streptococcus pneumoniae D39

63.797

98.693

0.63
  radA Streptococcus pneumoniae R6

63.797

98.693

0.63
  radA Streptococcus pneumoniae TIGR4

63.797

98.693

0.63
  radA Streptococcus mitis NCTC 12261

63.797

98.693

0.63
  radA Streptococcus mitis SK321

63.576

98.693

0.627