Detailed information NTG0931898

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: V2195_RS16195
• Genome accession: NZ_CP143903
• Coordinates: 3421931..3423079 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain PA13_L17_27.22_ST207
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3416931..3428079

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V2195_RS16170 (V2195_16170)	-	3418082..3418378 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
V2195_RS16175 (V2195_16175)	-	3418375..3418968 (-)	594	WP_003084544.1	YggT family protein	-
V2195_RS16180 (V2195_16180)	proC	3418979..3419800 (-)	822	WP_023092309.1	pyrroline-5-carboxylate reductase	-
V2195_RS16185 (V2195_16185)	-	3419812..3420504 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
V2195_RS16190 (V2195_16190)	pilT	3420719..3421753 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
V2195_RS16195 (V2195_16195)	pilU	3421931..3423079 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
V2195_RS16200 (V2195_16200)	czcD	3423087..3423986 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
V2195_RS16205 (V2195_16205)	-	3424141..3424545 (+)	405	WP_003084560.1	NINE protein	-
V2195_RS16210 (V2195_16210)	-	3424788..3426161 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
V2195_RS16215 (V2195_16215)	-	3426158..3427342 (+)	1185	WP_023092310.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=931898 V2195_RS16195 WP_003110016.1 3421931..3423079(+) (pilU) [Pseudomonas aeruginosa strain PA13_L17_27.22_ST207]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=931898 V2195_RS16195 WP_003110016.1 3421931..3423079(+) (pilU) [Pseudomonas aeruginosa strain PA13_L17_27.22_ST207]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382