Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   V2I11_RS12175 Genome accession   NZ_CP143783
Coordinates   2523930..2524544 (-) Length   204 a.a.
NCBI ID   WP_032873955.1    Uniprot ID   -
Organism   Bacillus velezensis strain JSZ06     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2518930..2529544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V2I11_RS12145 rpsT 2519233..2519499 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  V2I11_RS12150 holA 2519516..2520557 (-) 1042 Protein_2342 DNA polymerase III subunit delta -
  V2I11_RS12155 - 2520597..2520748 (-) 152 Protein_2343 hypothetical protein -
  V2I11_RS12160 - 2520789..2520923 (+) 135 WP_003152870.1 YqzM family protein -
  V2I11_RS12165 comEC 2520942..2523293 (-) 2352 WP_032873958.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  V2I11_RS12170 - 2523294..2523863 (-) 570 WP_003152868.1 ComE operon protein 2 -
  V2I11_RS12175 comEA 2523930..2524544 (-) 615 WP_032873955.1 helix-hairpin-helix domain-containing protein Machinery gene
  V2I11_RS12180 comER 2524603..2525424 (+) 822 WP_012118027.1 late competence protein ComER -
  V2I11_RS12185 - 2525493..2526230 (-) 738 WP_032873951.1 class I SAM-dependent DNA methyltransferase -
  V2I11_RS12190 rsfS 2526227..2526583 (-) 357 WP_007408260.1 ribosome silencing factor -
  V2I11_RS12195 yqeK 2526587..2527162 (-) 576 WP_032873949.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  V2I11_RS12200 - 2527152..2527721 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  V2I11_RS12205 yhbY 2527732..2528022 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  V2I11_RS12210 aroE 2528016..2528858 (-) 843 WP_032873946.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22265.47 Da        Isoelectric Point: 6.4940

>NTDB_id=931413 V2I11_RS12175 WP_032873955.1 2523930..2524544(-) (comEA) [Bacillus velezensis strain JSZ06]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDLKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=931413 V2I11_RS12175 WP_032873955.1 2523930..2524544(-) (comEA) [Bacillus velezensis strain JSZ06]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATTTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAACGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA Latilactobacillus sakei subsp. sakei 23K

31.897

100

0.363