Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   MWM16_RS04145 Genome accession   NZ_AP025617
Coordinates   740567..741037 (-) Length   156 a.a.
NCBI ID   WP_244345082.1    Uniprot ID   -
Organism   Thermus thermophilus strain SNM7-6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 735567..746037
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWM16_RS04120 (TthSNM76_08040) - 736820..737194 (-) 375 WP_244345078.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MWM16_RS04125 (TthSNM76_08050) - 737467..738966 (-) 1500 WP_244345079.1 pilus assembly PilX N-terminal domain-containing protein -
  MWM16_RS04130 (TthSNM76_08060) - 738980..739690 (-) 711 WP_011228525.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MWM16_RS04135 (TthSNM76_08070) - 739687..740019 (-) 333 WP_244345080.1 prepilin -
  MWM16_RS04140 (TthSNM76_08080) - 740045..740482 (-) 438 WP_244345081.1 GspH/FimT family protein -
  MWM16_RS04145 (TthSNM76_08090) pilA/pilA1 740567..741037 (-) 471 WP_244345082.1 GspH/FimT family protein Machinery gene
  MWM16_RS04150 (TthSNM76_08100) - 741195..742175 (+) 981 WP_244345083.1 YpdA family putative bacillithiol disulfide reductase -
  MWM16_RS04155 (TthSNM76_08110) ilvB 742331..744019 (+) 1689 WP_244345084.1 biosynthetic-type acetolactate synthase large subunit -
  MWM16_RS04160 (TthSNM76_08120) ilvN 744016..744528 (+) 513 WP_011173280.1 acetolactate synthase small subunit -
  MWM16_RS04165 (TthSNM76_08130) ilvC 744525..745538 (+) 1014 WP_244345085.1 ketol-acid reductoisomerase -

Sequence

Protein


Download         Length: 156 a.a.        Molecular weight: 16908.68 Da        Isoelectric Point: 11.5069

>NTDB_id=93062 MWM16_RS04145 WP_244345082.1 740567..741037(-) (pilA/pilA1) [Thermus thermophilus strain SNM7-6]
MTRRGLTLLELLLVLGILGVLLGLALPLLSPNRLALDQAARSLAAQVTRARLEAIRRNAFVGLQVFTEGAGGYLLFVDQN
ANRRYDPGEEFGATRFGQGDWARVRLDPEKSALGNMPLFFDPRGIPAKPITATLVLTSGGATRKVVISQQGRARLE

Nucleotide


Download         Length: 471 bp        

>NTDB_id=93062 MWM16_RS04145 WP_244345082.1 740567..741037(-) (pilA/pilA1) [Thermus thermophilus strain SNM7-6]
ATGACGCGGCGGGGCCTTACCCTTTTGGAGCTCCTGCTGGTCTTGGGCATCCTGGGGGTCCTCCTAGGGCTGGCCCTTCC
ACTTCTTTCCCCCAACCGCCTCGCCCTGGACCAGGCCGCCCGCTCCCTCGCCGCCCAGGTGACCCGGGCAAGGCTCGAGG
CCATCCGCCGGAACGCCTTCGTGGGCCTTCAGGTGTTCACCGAAGGAGCGGGGGGTTACTTGCTCTTCGTGGACCAAAAC
GCCAACCGCCGCTACGACCCCGGGGAGGAGTTCGGCGCGACCCGCTTCGGCCAAGGGGACTGGGCCCGGGTGCGCCTGGA
CCCGGAGAAGAGCGCCCTCGGCAACATGCCCCTTTTCTTTGACCCCCGGGGCATCCCGGCCAAGCCCATCACCGCCACGC
TCGTCCTCACCTCAGGGGGCGCCACCCGCAAGGTGGTCATCTCCCAGCAGGGCCGGGCCCGCCTGGAGTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-26)

Type II transport protein GspH (GspH)

(39-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Thermus thermophilus HB27

97.436

100

0.974

Multiple sequence alignment