Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   V1577_RS00395 Genome accession   NZ_CP143681
Coordinates   78146..79579 (+) Length   477 a.a.
NCBI ID   WP_028014571.1    Uniprot ID   A0AAU7N964
Organism   Enterobacter bugandensis strain ECC-209     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 73146..84579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1577_RS00365 (V1577_00360) - 73713..74690 (+) 978 WP_028014566.1 calcium/sodium antiporter -
  V1577_RS00370 (V1577_00365) kdsD 74704..75690 (+) 987 WP_028014567.1 arabinose-5-phosphate isomerase KdsD -
  V1577_RS00375 (V1577_00370) kdsC 75705..76271 (+) 567 WP_025756695.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  V1577_RS00380 (V1577_00375) lptC 76268..76843 (+) 576 WP_028014568.1 LPS export ABC transporter periplasmic protein LptC -
  V1577_RS00385 (V1577_00380) lptA 76812..77366 (+) 555 WP_028014569.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  V1577_RS00390 (V1577_00385) lptB 77373..78098 (+) 726 WP_028014570.1 LPS export ABC transporter ATP-binding protein -
  V1577_RS00395 (V1577_00390) rpoN/rpoN1 78146..79579 (+) 1434 WP_028014571.1 RNA polymerase factor sigma-54 Machinery gene
  V1577_RS00400 (V1577_00395) hpf 79602..79889 (+) 288 WP_014171818.1 ribosome hibernation promoting factor -
  V1577_RS00405 (V1577_00400) ptsN 79972..80463 (+) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  V1577_RS00410 (V1577_00405) rapZ 80509..81363 (+) 855 WP_014885397.1 RNase adapter RapZ -
  V1577_RS00415 (V1577_00410) npr 81360..81632 (+) 273 WP_025756690.1 PTS phosphocarrier protein NPr -
  V1577_RS00420 (V1577_00415) mtgA 81671..82396 (-) 726 WP_045630731.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  V1577_RS00425 (V1577_00420) elbB 82393..83046 (-) 654 WP_333043500.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54181.95 Da        Isoelectric Point: 4.3173

>NTDB_id=930591 V1577_RS00395 WP_028014571.1 78146..79579(+) (rpoN/rpoN1) [Enterobacter bugandensis strain ECC-209]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQADLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDEEIEIEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=930591 V1577_RS00395 WP_028014571.1 78146..79579(+) (rpoN/rpoN1) [Enterobacter bugandensis strain ECC-209]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGCTGGCGATGACGCCGCAGTTACAGCAGGCAATTCGTCTGTTGCA
ACTGTCCACCTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCCCTGCTGGAGCAAGCCGATCTTCATG
ACGAGGTAGATACCCAGCAGTCACAGGACACAGAAGCACTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCGGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAAATCTATACCGCCGGGACCCCTTCCGGCACGCGTGCAGACTACCAGGACGA
CGAGCTACCGGTCTACCAGGGCGAAACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTAGAGCTTACCCCCTTCT
CCGATACCGACCGCGCCATTGCCACCTCGATTGTCGACGCCGTTGATGACACCGGTTATTTGACCGTCACGCTGGACGAC
ATTCTGGAAAGCATGGGCGATGAAGAGATTGAGATTGAGGAGATTGAAGCGGTACTGAAACGCATTCAGCGCTTCGATCC
GGTCGGGGTGGCCGCTAAAGACCTGCGCGACTGTCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCGTGGCTCG
ACGAGGCACGCTTAATCATCAGCGATCATCTGGATCTTCTGGCTAACCACGATTTCCGCACCCTGATGCGCGTGACGCGC
CTGAAGGAAGAGGTGCTGAAAGAGGCGGTAAATCTGATCCAGTCCCTCGATCCGCGTCCCGGTCAGTCGATCCAGACCAG
CGAACCCGAGTACGTTATCCCGGACGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCTGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTACGCCTCCATGTGTACCAGCGCGCGCAACGACTCCGACAATCAATATATTCGC
AGCAATCTTCAGGAAGCGCGATGGTTGATCAAGAGCCTGGAGAGCCGGAATGATACCCTGCTTCGCGTGAGCCGCTGCAT
CGTCGAACAGCAGCAGGCCTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCCACTATTTCCCGCGTTACTACGCAGAAGTATCTGCACAGTCCGCGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAGGGTGGCGGCGAAGCCTCCTCAACGGCGATTCGCGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTAAGTGACAGTAAGTTGACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48