Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   V1603_RS00340 Genome accession   NZ_CP143680
Coordinates   63003..64436 (+) Length   477 a.a.
NCBI ID   WP_028014571.1    Uniprot ID   A0AAU7N964
Organism   Enterobacter sp. ECC-219     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 58003..69436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1603_RS00310 (V1603_00305) - 58570..59547 (+) 978 WP_028014566.1 calcium/sodium antiporter -
  V1603_RS00315 (V1603_00310) kdsD 59561..60547 (+) 987 WP_163329752.1 arabinose-5-phosphate isomerase KdsD -
  V1603_RS00320 (V1603_00315) kdsC 60562..61128 (+) 567 WP_395313374.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  V1603_RS00325 (V1603_00320) lptC 61125..61700 (+) 576 WP_028014568.1 LPS export ABC transporter periplasmic protein LptC -
  V1603_RS00330 (V1603_00325) lptA 61669..62223 (+) 555 WP_028014569.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  V1603_RS00335 (V1603_00330) lptB 62230..62955 (+) 726 WP_028014570.1 LPS export ABC transporter ATP-binding protein -
  V1603_RS00340 (V1603_00335) rpoN/rpoN1 63003..64436 (+) 1434 WP_028014571.1 RNA polymerase factor sigma-54 Machinery gene
  V1603_RS00345 (V1603_00340) hpf 64459..64746 (+) 288 WP_014171818.1 ribosome hibernation promoting factor -
  V1603_RS00350 (V1603_00345) ptsN 64829..65320 (+) 492 WP_116343515.1 PTS IIA-like nitrogen regulatory protein PtsN -
  V1603_RS00355 (V1603_00350) rapZ 65366..66220 (+) 855 WP_014885397.1 RNase adapter RapZ -
  V1603_RS00360 (V1603_00355) npr 66217..66489 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  V1603_RS00365 (V1603_00360) mtgA 66528..67253 (-) 726 WP_116343516.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  V1603_RS00370 (V1603_00365) elbB 67250..67903 (-) 654 WP_116343517.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54181.95 Da        Isoelectric Point: 4.3173

>NTDB_id=930567 V1603_RS00340 WP_028014571.1 63003..64436(+) (rpoN/rpoN1) [Enterobacter sp. ECC-219]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQADLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDEEIEIEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=930567 V1603_RS00340 WP_028014571.1 63003..64436(+) (rpoN/rpoN1) [Enterobacter sp. ECC-219]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGCTGGCGATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
GCTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCCCTGCTGGAGCAAGCCGATCTTCATG
ACGAGGTAGATACCCAGCAGTCACAGGATACAGAAGCGCTCGACACCGCGGATGCCCTCGAACAAAAAGAGATGCCGGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAAATCTATACCGCCGGGACCCCTTCCGGCACGCGTGCAGACTACCAGGACGA
CGAGCTACCGGTCTACCAGGGCGAAACCACCCAGTCGCTGCAGGATTACCTCATGTGGCAGGTAGAGCTTACCCCCTTCT
CCGATACCGACCGTGCCATTGCCACGTCGATTGTCGACGCCGTTGATGACACCGGTTATTTGACCGTCACGCTGGACGAC
ATTCTGGAAAGCATGGGCGATGAAGAGATTGAGATTGAGGAGATTGAAGCGGTACTGAAACGCATTCAGCGCTTCGATCC
GGTCGGGGTGGCCGCGAAAGACCTGCGCGACTGCCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCGTGGCTCG
ACGAAGCGCGCTTAATCATCAGCGATCATCTGGATCTTCTGGCTAACCACGATTTCCGCACCCTGATGCGCGTGACGCGC
CTGAAGGAAGAGGTGCTGAAAGAGGCGGTAAATCTGATCCAGTCTCTCGATCCGCGTCCCGGTCAGTCGATCCAGACCAG
CGAACCCGAGTACGTTATCCCGGACGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCTGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTACGCCTCTATGTGCACCAGCGCGCGCAACGACTCCGACAATCAATATATTCGC
AGCAATCTTCAGGAAGCGCGATGGTTGATCAAGAGCCTGGAGAGCCGGAATGATACCCTGCTGCGCGTGAGCCGCTGCAT
CGTCGAACAGCAGCAGGCATTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCCACCATTTCCCGCGTCACCACGCAGAAGTATCTGCACAGTCCGCGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAACACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCGATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTAAGTGACAGTAAGTTGACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48