Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   V1587_RS00280 Genome accession   NZ_CP143660
Coordinates   48275..49708 (+) Length   477 a.a.
NCBI ID   WP_371899188.1    Uniprot ID   -
Organism   Enterobacter sp. ECC-249     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 43275..54708
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1587_RS00250 (V1587_00245) - 43842..44819 (+) 978 WP_023309354.1 calcium/sodium antiporter -
  V1587_RS00255 (V1587_00250) kdsD 44833..45819 (+) 987 WP_371899187.1 arabinose-5-phosphate isomerase KdsD -
  V1587_RS00260 (V1587_00255) kdsC 45834..46400 (+) 567 WP_023309356.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  V1587_RS00265 (V1587_00260) lptC 46397..46972 (+) 576 WP_023309357.1 LPS export ABC transporter periplasmic protein LptC -
  V1587_RS00270 (V1587_00265) lptA 46941..47495 (+) 555 WP_023309358.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  V1587_RS00275 (V1587_00270) lptB 47502..48227 (+) 726 WP_023309359.1 LPS export ABC transporter ATP-binding protein -
  V1587_RS00280 (V1587_00275) rpoN/rpoN1 48275..49708 (+) 1434 WP_371899188.1 RNA polymerase factor sigma-54 Machinery gene
  V1587_RS00285 (V1587_00280) hpf 49731..50018 (+) 288 WP_008501444.1 ribosome hibernation promoting factor -
  V1587_RS00290 (V1587_00285) ptsN 50101..50592 (+) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  V1587_RS00295 (V1587_00290) rapZ 50638..51492 (+) 855 WP_014885397.1 RNase adapter RapZ -
  V1587_RS00300 (V1587_00295) npr 51489..51761 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  V1587_RS00305 (V1587_00300) mtgA 51801..52526 (-) 726 WP_371899189.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  V1587_RS00310 (V1587_00305) elbB 52523..53176 (-) 654 WP_371899220.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54111.86 Da        Isoelectric Point: 4.3332

>NTDB_id=930456 V1587_RS00280 WP_371899188.1 48275..49708(+) (rpoN/rpoN1) [Enterobacter sp. ECC-249]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDDEIELEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=930456 V1587_RS00280 WP_371899188.1 48275..49708(+) (rpoN/rpoN1) [Enterobacter sp. ECC-249]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCAATGACGCCGCAGCTACAGCAGGCAATTCGCCTGCTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGACACTCAGCAGTCACAGGATACAGAAGCGCTCGACACTGCGGATGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTTCCGCTTGATGCCAGCTGGGATGAAATCTACACGGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGACGA
TGAGCTACCGGTCTATCAGGGCGAAACCACCCAGTCGCTGCAGGATTACCTTATGTGGCAGGTGGAACTTACCCCCTTCT
CCGATACCGACCGCGCGATTGCCACGTCAATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTTACGCTGGACGAT
ATTCTGGAAAGCATGGGCGACGATGAGATTGAGCTTGAGGAAGTTGAAGCCGTTCTGAAGCGCATTCAGCGTTTCGATCC
GGTAGGCGTAGCCGCAAAAGATCTGCGCGACTGCCTGCTGATCCAGCTCTCTCAGTTCAGCAAAGAGACGCCATGGCTCG
ACGAGGCGCGCTTAATCATCAGCGATCATCTGGATCTGCTGGCAAACCACGATTTCCGCAGCCTGATGCGCGTCACGCGC
CTGAAGGAAGAGGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGCCCCGGTCAGTCGATCCAGACCAG
CGAACCCGAGTACGTGATCCCGGATGTGCTGGTCAGAAAACACAACGGCCGCTGGGTAGTTGAACTGAATTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCGCGTAACGACTCCGACAATCAATACATTCGT
AGCAATCTTCAGGAAGCGCGATGGCTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTACGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATATTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48