Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   V1573_RS00280 Genome accession   NZ_CP143658
Coordinates   48289..49722 (+) Length   477 a.a.
NCBI ID   WP_047060252.1    Uniprot ID   -
Organism   Enterobacter asburiae strain ECC-253     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 43289..54722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1573_RS00250 (V1573_00250) - 43856..44833 (+) 978 WP_048981182.1 calcium/sodium antiporter -
  V1573_RS00255 (V1573_00255) kdsD 44847..45833 (+) 987 WP_032659705.1 arabinose-5-phosphate isomerase KdsD -
  V1573_RS00260 (V1573_00260) kdsC 45848..46414 (+) 567 WP_048981180.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  V1573_RS00265 (V1573_00265) lptC 46411..46986 (+) 576 WP_033146749.1 LPS export ABC transporter periplasmic protein LptC -
  V1573_RS00270 (V1573_00270) lptA 46955..47509 (+) 555 WP_033146750.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  V1573_RS00275 (V1573_00275) lptB 47516..48241 (+) 726 WP_033146751.1 LPS export ABC transporter ATP-binding protein -
  V1573_RS00280 (V1573_00280) rpoN/rpoN1 48289..49722 (+) 1434 WP_047060252.1 RNA polymerase factor sigma-54 Machinery gene
  V1573_RS00285 (V1573_00285) hpf 49745..50032 (+) 288 WP_008501444.1 ribosome hibernation promoting factor -
  V1573_RS00290 (V1573_00290) ptsN 50115..50606 (+) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  V1573_RS00295 (V1573_00295) rapZ 50652..51506 (+) 855 WP_014885397.1 RNase adapter RapZ -
  V1573_RS00300 (V1573_00300) npr 51503..51775 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  V1573_RS00305 (V1573_00305) mtgA 51815..52540 (-) 726 WP_048981179.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  V1573_RS00310 (V1573_00310) elbB 52537..53190 (-) 654 WP_039260518.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54197.95 Da        Isoelectric Point: 4.3173

>NTDB_id=930434 V1573_RS00280 WP_047060252.1 48289..49722(+) (rpoN/rpoN1) [Enterobacter asburiae strain ECC-253]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDEEIELEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=930434 V1573_RS00280 WP_047060252.1 48289..49722(+) (rpoN/rpoN1) [Enterobacter asburiae strain ECC-253]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCAATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACTCAGCAGTCACAGGATACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTATCAGGGAGAAACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAACTTACCCCCTTCT
CCGATACCGACCGCGCGATTGCCACCTCAATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTGGACGAT
ATCCTGGAAAGCATGGGCGACGAAGAGATTGAACTTGAGGAGATCGAAGCCGTTCTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCATGGCTCG
ACGAGGCGCGCTTAATCATCAGCGATCATCTGGATCTGTTGGCCAACCACGATTTCCGCAGCCTGATGCGCGTCACGCGC
CTGAAGGAAGAGGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCACTCGATCCGCGCCCGGGACAGTCGATCCAGACCAG
CGAGCCCGAGTACGTCATCCCGGACGTGCTGGTGAGAAAACACAATGACCGCTGGGTCGTTGAACTGAATTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCGCGTAACGACTCCGACAATCAATATATTCGT
AGCAATCTTCAGGAAGCGCGATGGCTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATATTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.545

100

0.482