Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   V1586_RS00280 Genome accession   NZ_CP143650
Coordinates   48291..49724 (+) Length   477 a.a.
NCBI ID   WP_025756691.1    Uniprot ID   A0A0F1BDQ8
Organism   Enterobacter sichuanensis strain ECC-263     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 43291..54724
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1586_RS00250 (V1586_00245) - 43858..44835 (+) 978 WP_045284743.1 calcium/sodium antiporter -
  V1586_RS00255 (V1586_00250) kdsD 44849..45835 (+) 987 WP_025756696.1 arabinose-5-phosphate isomerase KdsD -
  V1586_RS00260 (V1586_00255) kdsC 45850..46416 (+) 567 WP_059385405.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  V1586_RS00265 (V1586_00260) lptC 46413..46988 (+) 576 WP_047959546.1 LPS export ABC transporter periplasmic protein LptC -
  V1586_RS00270 (V1586_00265) lptA 46957..47511 (+) 555 WP_025756693.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  V1586_RS00275 (V1586_00270) lptB 47518..48243 (+) 726 WP_025756692.1 LPS export ABC transporter ATP-binding protein -
  V1586_RS00280 (V1586_00275) rpoN/rpoN1 48291..49724 (+) 1434 WP_025756691.1 RNA polymerase factor sigma-54 Machinery gene
  V1586_RS00285 (V1586_00280) hpf 49747..50034 (+) 288 WP_013098943.1 ribosome hibernation promoting factor -
  V1586_RS00290 (V1586_00285) ptsN 50117..50608 (+) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  V1586_RS00295 (V1586_00290) rapZ 50654..51508 (+) 855 WP_014885397.1 RNase adapter RapZ -
  V1586_RS00300 (V1586_00295) npr 51505..51777 (+) 273 WP_025756690.1 PTS phosphocarrier protein NPr -
  V1586_RS00305 (V1586_00300) mtgA 51817..52542 (-) 726 WP_095441913.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  V1586_RS00310 (V1586_00305) elbB 52539..53192 (-) 654 WP_025756688.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54195.98 Da        Isoelectric Point: 4.3358

>NTDB_id=930389 V1586_RS00280 WP_025756691.1 48291..49724(+) (rpoN/rpoN1) [Enterobacter sichuanensis strain ECC-263]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDEEIELEEVEAVLKRIQRFDPVGVAARDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNSDSIPRLQINQQYASMCTSVRNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=930389 V1586_RS00280 WP_025756691.1 48291..49724(+) (rpoN/rpoN1) [Enterobacter sichuanensis strain ECC-263]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCAATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAACTCCAGCAGGCGCTGGACAGTAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACCCAGCAATCGCAGGATACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCGGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAGATCTACACCGCCGGAACCCCATCCGGAACGCGTGCGGACTACCAGGACGA
TGAGCTACCGGTCTATCAGGGAGAAACCACTCAGTCGCTTCAGGATTACCTGATGTGGCAGGTGGAACTGACCCCCTTCT
CCGACACCGATCGTGCGATTGCCACATCCATTGTCGATGCCGTTGATGACACAGGCTATTTAACCGTCACGCTGGACGAC
ATCCTCGAAAGCATGGGCGACGAAGAGATTGAGCTTGAGGAGGTTGAAGCCGTTTTGAAGCGCATTCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAGAGATCTGCGCGACTGCCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCGTGGCTTG
ATGAGGCGCGCTTAATCATCAGCGATCATCTGGATCTGTTGGCTAACCACGATTTCCGCACCCTGATGCGCGTCACGCGG
CTGAAAGAAGATGTGCTGAAAGAAGCGGTGAATCTCATCCAGTCGCTCGATCCGCGCCCCGGTCAGTCGATCCAGACCAG
CGAACCCGAATACGTGATCCCGGATGTGCTGGTCCGAAAACACAATGGCCGCTGGGTCGTTGAACTGAATTCTGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGTGCGTAACGACTCCGACAATCAATATATTCGT
AGCAATTTGCAGGAAGCGCGATGGTTGATCAAAAGTCTGGAGAGCCGTAATGATACGCTGCTTCGCGTCAGCCGCTGCAT
TGTAGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTCAATACCGAGGGTGGCGGCGAAGCGTCGTCAACCGCGATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTGAGTGACAGTAAGTTAACCACCATGCTGTCCGAACAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F1BDQ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.941

100

0.486