Detailed information NTG0930291

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QAA72_RS02435
• Genome accession: NZ_CP143647
• Coordinates: 513207..514355 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain CPO180
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 508207..519355

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QAA72_RS02410 (QAA72_002410)	-	509358..509654 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
QAA72_RS02415 (QAA72_002415)	-	509651..510244 (-)	594	WP_003084544.1	YggT family protein	-
QAA72_RS02420 (QAA72_002420)	proC	510255..511076 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
QAA72_RS02425 (QAA72_002425)	-	511088..511780 (-)	693	WP_015649764.1	YggS family pyridoxal phosphate-dependent enzyme	-
QAA72_RS02430 (QAA72_002430)	pilT	511995..513029 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
QAA72_RS02435 (QAA72_002435)	pilU	513207..514355 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
QAA72_RS02440 (QAA72_002440)	czcD	514363..515262 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
QAA72_RS02445 (QAA72_002445)	-	515416..515820 (+)	405	WP_003084560.1	NINE protein	-
QAA72_RS02450 (QAA72_002450)	-	516063..517436 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
QAA72_RS02455 (QAA72_002455)	-	517433..518617 (+)	1185	WP_015649763.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=930291 QAA72_RS02435 WP_003100959.1 513207..514355(+) (pilU) [Pseudomonas aeruginosa strain CPO180]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=930291 QAA72_RS02435 WP_003100959.1 513207..514355(+) (pilU) [Pseudomonas aeruginosa strain CPO180]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382