Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA2   Type   Machinery gene
Locus tag   MWM12_RS06475 Genome accession   NZ_AP025603
Coordinates   1179916..1180497 (+) Length   193 a.a.
NCBI ID   WP_244340525.1    Uniprot ID   -
Organism   Thermus thermophilus strain SNM1-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1174916..1185497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWM12_RS06455 (TthSNM17_12640) ilvN 1175913..1176425 (-) 513 WP_011173280.1 acetolactate synthase small subunit -
  MWM12_RS06460 (TthSNM17_12650) ilvB 1176422..1178110 (-) 1689 WP_244340521.1 biosynthetic-type acetolactate synthase large subunit -
  MWM12_RS06465 (TthSNM17_12660) - 1178266..1179246 (-) 981 WP_244340523.1 YpdA family putative bacillithiol disulfide reductase -
  MWM12_RS06470 (TthSNM17_12670) pilA/pilA1 1179411..1179881 (+) 471 WP_165739610.1 GspH/FimT family protein Machinery gene
  MWM12_RS06475 (TthSNM17_12680) pilA/pilA2 1179916..1180497 (+) 582 WP_244340525.1 type II secretion system protein Machinery gene
  MWM12_RS06480 (TthSNM17_12690) pilA/pilA3 1180494..1181195 (+) 702 WP_244340527.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  MWM12_RS06485 (TthSNM17_12700) comZ 1181206..1182882 (+) 1677 WP_244340529.1 pilus assembly PilX N-terminal domain-containing protein Machinery gene
  MWM12_RS06490 (TthSNM17_12710) - 1183105..1183545 (+) 441 WP_244340531.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MWM12_RS06495 (TthSNM17_12720) - 1183618..1183977 (+) 360 WP_244340533.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MWM12_RS06500 (TthSNM17_12730) - 1184215..1184694 (+) 480 WP_244340535.1 DUF1640 domain-containing protein -
  MWM12_RS06505 - 1184770..1185307 (+) 538 Protein_1259 Uma2 family endonuclease -

Sequence

Protein


Download         Length: 193 a.a.        Molecular weight: 20449.51 Da        Isoelectric Point: 9.4639

>NTDB_id=92985 MWM12_RS06475 WP_244340525.1 1179916..1180497(+) (pilA/pilA2) [Thermus thermophilus strain SNM1-7]
MRKGLTLVEVLIALAVLGVAFGALLMSQVSNLRANAQARFATDAKAAAVQVLERRSAEVLKSEIVPALSPYKDAPLDPDN
PSGNWRSFYFVDYYFSCPTRVAPSPKQRGGSVANLRPGLTCSGTETVFGIPVAWSIRGESGILGEGVVTVVVTATHPRGP
KVTLGRRVTCYDVYPSPTQDQPAPCPPPGGGRP

Nucleotide


Download         Length: 582 bp        

>NTDB_id=92985 MWM12_RS06475 WP_244340525.1 1179916..1180497(+) (pilA/pilA2) [Thermus thermophilus strain SNM1-7]
ATGAGAAAAGGTCTTACCCTCGTGGAAGTCCTCATCGCCTTAGCCGTCTTGGGAGTGGCCTTCGGCGCTCTCCTTATGAG
CCAGGTTTCCAATCTCAGGGCGAACGCCCAGGCCCGCTTCGCCACCGACGCCAAGGCCGCCGCCGTCCAGGTTCTGGAGC
GCCGCTCGGCCGAGGTGCTGAAAAGCGAAATCGTCCCGGCCTTAAGCCCCTACAAGGACGCCCCCTTGGACCCGGACAAC
CCTTCGGGAAACTGGCGGAGCTTTTACTTCGTGGACTACTACTTCTCCTGCCCCACCCGGGTGGCCCCTTCCCCTAAGCA
GAGGGGAGGGTCTGTAGCCAACCTTCGTCCCGGCCTCACCTGCTCGGGCACGGAGACGGTCTTTGGCATCCCCGTGGCCT
GGAGCATCCGCGGGGAAAGCGGCATCCTGGGCGAGGGCGTGGTGACGGTGGTGGTCACGGCCACCCATCCCCGGGGGCCC
AAGGTCACCCTGGGCCGCCGGGTCACCTGCTACGACGTCTACCCTTCCCCCACCCAGGACCAGCCCGCCCCTTGCCCGCC
CCCGGGAGGTGGTCGCCCATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA2 Thermus thermophilus HB27

92.432

95.855

0.886

Multiple sequence alignment