Detailed information NTG0929651

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: V1241_RS25410
• Genome accession: NZ_CP143552
• Coordinates: 3129238..3130380 (+)
• Length: 380 a.a.
• NCBI ID: WP_006416170.1
• Uniprot ID: A0A0H3KRG3
• Organism: Burkholderia multivorans strain 2023CK-01339
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3124238..3135380

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V1241_RS25380 (V1241_25385)	-	3124633..3125238 (+)	606	WP_012212698.1	50S ribosomal protein L25/general stress protein Ctc	-
V1241_RS25385 (V1241_25390)	pth	3125347..3125946 (+)	600	WP_006398241.1	aminoacyl-tRNA hydrolase	-
V1241_RS25390 (V1241_25395)	hisC	3126083..3127150 (+)	1068	WP_155823182.1	histidinol-phosphate transaminase	-
V1241_RS25395 (V1241_25400)	-	3127217..3127480 (-)	264	WP_006406632.1	YfhL family 4Fe-4S dicluster ferredoxin	-
V1241_RS25400 (V1241_25405)	coaD	3127801..3128298 (-)	498	WP_006398238.1	pantetheine-phosphate adenylyltransferase	-
V1241_RS25405 (V1241_25410)	rsmD	3128410..3129024 (-)	615	WP_039220118.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
V1241_RS25410 (V1241_25415)	pilA	3129238..3130380 (+)	1143	WP_006416170.1	signal recognition particle-docking protein FtsY	Machinery gene
V1241_RS25415 (V1241_25420)	maiA	3130451..3131095 (-)	645	WP_006416174.1	maleylacetoacetate isomerase	-
V1241_RS25420 (V1241_25425)	ybiB	3131269..3132240 (+)	972	WP_155823183.1	DNA-binding protein YbiB	-
V1241_RS25425 (V1241_25430)	leuA	3132633..3134279 (+)	1647	WP_155823184.1	2-isopropylmalate synthase	-
V1241_RS25430 (V1241_25435)	-	3134415..3134798 (-)	384	WP_006398232.1	nuclear transport factor 2 family protein	-

Sequence

Protein

• Length: 380 a.a.
• Molecular weight: 40336.19 Da
• Isoelectric Point: 4.9462

>NTDB_id=929651 V1241_RS25410 WP_006416170.1 3129238..3130380(+) (pilA) [Burkholderia multivorans strain 2023CK-01339]
MFSFFKRFKKTQEPGSAESQPADAPQPDAPSDAHDADVPPAPAPAEPAAPAVVMTVTPSDNGRDEVVETVEIVPPPVQDA
SAKKSWLARLKTGLAKTGSSITGVFVNTKIDDALYEELETALLMSDAGVDATEYLLGALREKVRVGRLTDPQQVKSALRD
LLVELLTPLEKSLVLGRAQPLVMMIAGVNGAGKTTSIGKLAKHLQSFDQSVLLAAGDTFRAAAREQLTVWGERNNVTVVQ
QESGDPAAVIFDAVSAARARKIDVMMADTAGRLPTQLHLMEELKKVKRVIAKAHDGAPHEVLLVIDANTGQNALTQVKAF
DDALGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVGEKVEDLQPFSAEEFADALLG

Nucleotide

• Length: 1143 bp

>NTDB_id=929651 V1241_RS25410 WP_006416170.1 3129238..3130380(+) (pilA) [Burkholderia multivorans strain 2023CK-01339]
ATGTTCAGCTTCTTCAAACGATTCAAGAAAACGCAGGAGCCCGGCTCCGCGGAATCGCAGCCGGCCGACGCGCCGCAACC
CGACGCCCCGTCCGACGCGCACGACGCCGACGTTCCGCCTGCACCCGCGCCTGCCGAACCGGCCGCGCCCGCCGTCGTGA
TGACGGTCACGCCGAGCGACAACGGCCGCGACGAAGTCGTCGAGACCGTCGAAATCGTGCCGCCGCCCGTGCAGGATGCA
TCGGCGAAGAAATCGTGGCTCGCGCGCCTGAAGACGGGCCTCGCGAAAACGGGCTCGAGCATCACCGGCGTATTCGTGAA
CACGAAGATCGACGACGCGCTGTACGAGGAGCTCGAAACCGCGCTGCTGATGTCCGATGCCGGCGTCGACGCGACCGAGT
ACCTGCTCGGCGCGCTGCGCGAAAAGGTGCGGGTGGGCCGCCTGACCGACCCGCAGCAGGTGAAGAGCGCGCTGCGCGAC
CTGCTCGTCGAACTGCTGACGCCGCTCGAGAAGTCGCTCGTGCTCGGCCGTGCGCAGCCGCTCGTGATGATGATCGCGGG
CGTCAACGGCGCCGGCAAGACGACCAGCATCGGCAAGCTGGCGAAGCATCTACAGAGCTTCGACCAGTCGGTGCTGCTCG
CCGCGGGCGATACGTTCCGCGCCGCGGCGCGCGAACAGCTGACGGTCTGGGGCGAACGCAACAACGTGACCGTCGTGCAG
CAGGAAAGCGGCGACCCGGCCGCGGTGATCTTCGACGCGGTCAGCGCCGCGCGCGCGCGCAAGATCGACGTGATGATGGC
CGACACGGCCGGCCGCCTGCCGACGCAGCTTCATCTGATGGAAGAGCTGAAGAAGGTGAAGCGCGTGATCGCGAAGGCAC
ACGACGGCGCGCCGCACGAAGTGCTGCTCGTGATCGACGCGAACACCGGTCAGAACGCGCTCACGCAGGTGAAGGCATTC
GACGATGCGCTCGGCCTGACGGGCCTCATCGTCACGAAGCTCGACGGCACCGCGAAGGGCGGGATTCTCGCCGCGATCGC
GCGGCAGCGGCCGGTGCCCGTGTACTTCATCGGCGTCGGCGAAAAGGTCGAGGATCTGCAGCCGTTCAGCGCCGAGGAAT
TCGCGGACGCGCTGCTCGGCTGA

Domains

Predicted by InterproScan.

• SRP54-type protein, helical bundle domain (SRP54_N) (98-160)

• SRP54-type protein, GTPase domain (SRP54) (181-380)

3D structure

• AlphaFold DB: A0A0H3KRG3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	53.846	100	0.589