Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilW   Type   Machinery gene
Locus tag   MWM06_RS08860 Genome accession   NZ_AP025596
Coordinates   1604554..1605432 (+) Length   292 a.a.
NCBI ID   WP_244360456.1    Uniprot ID   -
Organism   Thermus thermophilus strain SNM1-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1599554..1610432
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWM06_RS08835 (TthSNM11_17740) - 1600548..1601039 (+) 492 WP_011173430.1 DUF5317 domain-containing protein -
  MWM06_RS08840 (TthSNM11_17750) - 1601094..1602098 (+) 1005 WP_244340638.1 homoisocitrate dehydrogenase -
  MWM06_RS08845 (TthSNM11_17760) pilM 1602237..1603370 (+) 1134 WP_011173432.1 type IV pilus assembly protein PilM Machinery gene
  MWM06_RS08850 (TthSNM11_17770) pilN 1603363..1603986 (+) 624 WP_011228637.1 competence protein Machinery gene
  MWM06_RS08855 (TthSNM11_17780) pilO 1603976..1604557 (+) 582 WP_011173434.1 type 4a pilus biogenesis protein PilO Machinery gene
  MWM06_RS08860 (TthSNM11_17790) pilW 1604554..1605432 (+) 879 WP_244360456.1 competence protein Machinery gene
  MWM06_RS08865 (TthSNM11_17800) pilQ 1605429..1607702 (+) 2274 WP_244360459.1 secretin N-terminal domain-containing protein Machinery gene
  MWM06_RS08870 (TthSNM11_17810) aroC 1607761..1608912 (+) 1152 WP_011228640.1 chorismate synthase -
  MWM06_RS08875 (TthSNM11_17820) - 1608937..1609491 (+) 555 WP_008632876.1 shikimate kinase -

Sequence

Protein


Download         Length: 292 a.a.        Molecular weight: 29764.26 Da        Isoelectric Point: 4.5640

>NTDB_id=92963 MWM06_RS08860 WP_244360456.1 1604554..1605432(+) (pilW) [Thermus thermophilus strain SNM1-1]
MKNAWQRLKEAWANLPRSTKLLLAALLLVGGVALWYVGLYLPAQVAEAPTPAPSTQVIEAPPIPPLASQEEAKPQAEAPA
QEEAQAPASSQEEAQAPPPAPVARAEPPPPNPFVPLVVETPPPPPASASRPAPVPEGPAVRVQTPAPAPQAAPATRPIPG
TSGALPAPKILSPALSAPLPQARETPPRVAVPTALVEAPLSGPEAEGVKEAPAPASPLERLVAEKGLRLSGTLLGPVSVA
ILESKEGYLVVPVGSPIPGTEAVVRQVEEGSVTLALKEETLNLSLVQAGGGQ

Nucleotide


Download         Length: 879 bp        

>NTDB_id=92963 MWM06_RS08860 WP_244360456.1 1604554..1605432(+) (pilW) [Thermus thermophilus strain SNM1-1]
ATGAAGAACGCCTGGCAACGCCTGAAGGAAGCCTGGGCCAACCTGCCCAGGTCCACCAAGCTCCTCCTGGCGGCCCTCCT
TCTCGTGGGGGGCGTGGCCCTTTGGTACGTGGGCCTCTACCTCCCGGCCCAGGTGGCGGAAGCGCCCACCCCGGCCCCCT
CCACGCAGGTCATCGAGGCTCCCCCGATCCCGCCCCTCGCCTCCCAGGAAGAGGCCAAGCCACAGGCCGAAGCCCCGGCC
CAAGAGGAGGCCCAGGCGCCCGCTTCTTCCCAAGAGGAGGCCCAGGCGCCCCCGCCCGCCCCCGTGGCCCGGGCCGAGCC
CCCGCCCCCGAACCCCTTCGTCCCCCTGGTGGTGGAAACCCCACCCCCACCTCCGGCCTCCGCCTCCCGCCCGGCGCCCG
TCCCCGAGGGGCCCGCCGTCCGGGTCCAGACCCCTGCCCCTGCCCCCCAGGCGGCCCCCGCCACCCGGCCCATCCCCGGG
ACCTCCGGCGCCCTCCCGGCGCCCAAGATCCTCTCCCCCGCGCTGTCCGCCCCCCTGCCCCAGGCCAGGGAGACGCCGCC
TAGGGTCGCCGTGCCCACGGCGCTCGTGGAGGCCCCCCTTTCCGGGCCCGAGGCAGAAGGGGTGAAAGAAGCCCCCGCCC
CGGCCTCGCCGCTGGAGCGCCTGGTGGCGGAAAAGGGCCTCCGCCTCTCGGGGACCCTGCTCGGCCCCGTGAGCGTGGCC
ATCCTGGAGAGCAAGGAGGGCTACCTCGTGGTCCCTGTGGGAAGCCCCATCCCCGGCACGGAGGCCGTGGTGCGCCAGGT
GGAGGAAGGAAGCGTGACCCTGGCCTTGAAGGAGGAAACCTTGAACCTGTCCCTCGTCCAAGCTGGAGGTGGCCAATGA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilW Thermus thermophilus HB27

96.575

100

0.966

Multiple sequence alignment