Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   V1499_RS00610 Genome accession   NZ_CP143543
Coordinates   119100..120479 (+) Length   459 a.a.
NCBI ID   WP_409273018.1    Uniprot ID   -
Organism   Neobacillus sp. SCS-31     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 114100..125479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1499_RS00590 (V1499_00590) - 114387..114848 (+) 462 WP_316572510.1 CtsR family transcriptional regulator -
  V1499_RS00595 (V1499_00595) - 114896..115444 (+) 549 WP_409273013.1 UvrB/UvrC motif-containing protein -
  V1499_RS00600 (V1499_00600) - 115447..116520 (+) 1074 WP_409273014.1 protein arginine kinase -
  V1499_RS00605 (V1499_00605) clpC 116534..118972 (+) 2439 WP_409273016.1 ATP-dependent protease ATP-binding subunit ClpC -
  V1499_RS00610 (V1499_00610) radA 119100..120479 (+) 1380 WP_409273018.1 DNA repair protein RadA Machinery gene
  V1499_RS00615 (V1499_00615) - 120600..121682 (+) 1083 WP_409273020.1 PIN/TRAM domain-containing protein -
  V1499_RS00620 (V1499_00620) ispD 121740..122432 (+) 693 WP_409273022.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  V1499_RS00625 (V1499_00625) ispF 122478..122960 (+) 483 WP_409273024.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  V1499_RS00630 (V1499_00630) gltX 123045..124502 (+) 1458 WP_409273026.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49699.15 Da        Isoelectric Point: 7.1326

>NTDB_id=929444 V1499_RS00610 WP_409273018.1 119100..120479(+) (radA) [Neobacillus sp. SCS-31]
MAKRKTKFMCTNCGYESPKWMGKCPGCGEWNKMVEEVEPASTGRRGAFAHSEGSAVSSKAMPITAVETISEPRIYTEMTE
LNRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSSQLANKGHKVLYISGEESLRQTKLRAERLGVASENLLVYSETNLEEI
SRTIGQTAPEFVIVDSIQTVFHPEVTSAPGSVSQVRECTAELMRIGKTKGIAIFIVGHVTKEGNIAGPRLLEHMVDTVLY
FEGERHHTYRILRAVKNRFGSTNEMGIFEMKEAGLEEVENPSEIFLEERSRGAAGSTVVASMEGTRPVLVEIQALISPTS
FGNPRRMATGIDHNRVPLLMAVLEKRVGMLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDKPTRPTDCIIGEVGL
TGEVRRVSRIEQRVQEAAKLGFERVILPANNLGGWTGPKGLELIGVSTVSEALRAALGE

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=929444 V1499_RS00610 WP_409273018.1 119100..120479(+) (radA) [Neobacillus sp. SCS-31]
ATGGCAAAACGTAAAACGAAATTTATGTGCACGAATTGCGGATACGAATCGCCCAAGTGGATGGGAAAATGTCCTGGTTG
TGGGGAATGGAATAAAATGGTCGAAGAAGTAGAACCCGCCTCGACGGGCAGGCGAGGCGCATTTGCCCATTCCGAAGGTT
CGGCGGTTTCCTCAAAGGCTATGCCGATCACTGCAGTTGAAACAATTAGTGAACCAAGGATTTATACCGAAATGACGGAA
TTAAACAGAGTGCTTGGTGGCGGTGTTGTCAAAGGTTCGCTTGTACTTATAGGCGGCGATCCAGGAATCGGGAAATCGAC
CCTTCTCCTTCAGGTATCTTCCCAACTTGCAAATAAAGGACACAAAGTCCTATATATCTCAGGGGAAGAATCGCTCCGTC
AGACAAAGTTGCGGGCAGAGAGGCTTGGAGTTGCGTCAGAGAATCTTCTGGTGTACTCTGAAACCAATCTGGAAGAAATC
AGCAGGACAATCGGACAAACTGCTCCCGAATTCGTAATCGTTGATTCGATTCAGACCGTTTTTCACCCGGAGGTTACTTC
AGCGCCTGGAAGCGTTTCGCAGGTCAGGGAGTGTACAGCGGAGCTGATGAGAATTGGTAAAACAAAGGGAATTGCCATCT
TTATTGTCGGCCATGTCACAAAGGAAGGCAATATCGCCGGACCCAGGTTATTGGAGCATATGGTCGATACCGTACTTTAC
TTTGAAGGGGAACGTCACCATACGTACCGGATTCTCCGCGCTGTCAAAAACCGTTTTGGTTCCACGAATGAGATGGGAAT
TTTTGAAATGAAGGAAGCTGGCCTGGAGGAAGTCGAGAACCCATCTGAAATTTTTCTTGAGGAACGTTCACGCGGTGCGG
CAGGCTCTACGGTTGTTGCATCAATGGAAGGGACGAGGCCGGTGCTTGTTGAAATACAGGCGCTCATTTCACCGACTAGC
TTTGGTAATCCGAGGAGAATGGCTACGGGAATAGATCATAACAGGGTTCCACTTTTAATGGCAGTCCTCGAAAAACGGGT
TGGGATGCTCCTCCAGAACCAGGATGCTTATTTGAAGGTGGCTGGCGGCGTCAAATTGGATGAACCGGCCATAGACTTGG
CGGTTGCCGTGAGCATTGCATCCAGCTTTCGTGATAAACCGACAAGGCCGACAGACTGCATCATAGGCGAGGTTGGGCTC
ACAGGTGAGGTCAGGCGGGTATCAAGAATTGAGCAGCGCGTCCAGGAAGCGGCCAAGCTTGGTTTTGAGCGTGTCATTCT
TCCAGCGAATAACCTGGGGGGCTGGACAGGCCCGAAAGGCTTGGAGCTGATAGGAGTTTCCACTGTAAGTGAAGCGTTAA
GGGCTGCGCTTGGAGAATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-431)

AAA domain (AAA_25)

(74-223)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

76.688

100

0.767
  radA Streptococcus mitis NCTC 12261

63.319

99.782

0.632
  radA Streptococcus pneumoniae Rx1

63.319

99.782

0.632
  radA Streptococcus pneumoniae D39

63.319

99.782

0.632
  radA Streptococcus pneumoniae R6

63.319

99.782

0.632
  radA Streptococcus pneumoniae TIGR4

63.319

99.782

0.632
  radA Streptococcus mitis SK321

63.1

99.782

0.63