Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   V1497_RS00625 Genome accession   NZ_CP143541
Coordinates   118791..120161 (+) Length   456 a.a.
NCBI ID   WP_349409100.1    Uniprot ID   -
Organism   Pseudalkalibacillus sp. SCS-8     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 113791..125161
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1497_RS00605 (V1497_00605) - 114001..114465 (+) 465 WP_349409096.1 CtsR family transcriptional regulator -
  V1497_RS00610 (V1497_00610) - 114480..115007 (+) 528 WP_349409097.1 UvrB/UvrC motif-containing protein -
  V1497_RS00615 (V1497_00615) - 115010..116083 (+) 1074 WP_349409098.1 protein arginine kinase -
  V1497_RS00620 (V1497_00620) clpC 116097..118529 (+) 2433 WP_349409099.1 ATP-dependent protease ATP-binding subunit ClpC -
  V1497_RS00625 (V1497_00625) radA 118791..120161 (+) 1371 WP_349409100.1 DNA repair protein RadA Machinery gene
  V1497_RS00630 (V1497_00630) disA 120165..121238 (+) 1074 WP_349409101.1 DNA integrity scanning diadenylate cyclase DisA -
  V1497_RS00635 (V1497_00635) - 121373..122488 (+) 1116 WP_349409102.1 PIN/TRAM domain-containing protein -
  V1497_RS00640 (V1497_00640) ispD 122516..123214 (+) 699 WP_349409103.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  V1497_RS00645 (V1497_00645) ispF 123207..123680 (+) 474 WP_349409104.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49875.05 Da        Isoelectric Point: 7.7764

>NTDB_id=929350 V1497_RS00625 WP_349409100.1 118791..120161(+) (radA) [Pseudalkalibacillus sp. SCS-8]
MAKKKTKFLCQDCGYDSPKWMGKCPGCNKWNTMVEEIIQPDKGHRRSFQTGGSSQKPEPITKIKSEKEPRIDTKINELNR
VLGGGVVPGSLVLVGGDPGIGKSTLLLQASAKLAQSDQTVLYISGEESIKQTKLRAERLGINHDRLYVHAETDMDLIEKA
VQEVNPSLLIIDSIQTVYRSEIASAPGSVSQVRECTSQFMRISKTNGIATFIVGHVTKEGSIAGPRLLEHMVDAVLYFEG
ERHHTYRILRAVKNRFGSTNEIGVFEMKEEGLDEVQNPSEVFLEERSNGAAGSAIVASMEGTRPVLVEIQALVSPTSFGN
PRRMGNGIDHNRVSLLMAVLEKRVGLLMQNQDAYVNVAGGVRLDEPAIDLAIAVSIASSFRDQPTNPTDVYIGEIGLTGE
VRRVSRVEQRVHEVAKLGFKRVILPEKNLGGWSAPKGIEIVGVSSVQETLRHTLGG

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=929350 V1497_RS00625 WP_349409100.1 118791..120161(+) (radA) [Pseudalkalibacillus sp. SCS-8]
ATGGCGAAGAAAAAAACAAAGTTCTTATGTCAGGATTGTGGATATGATTCACCTAAATGGATGGGAAAGTGCCCAGGGTG
TAATAAGTGGAATACGATGGTCGAAGAAATCATCCAGCCAGATAAAGGACATAGACGCTCGTTTCAAACTGGCGGTTCCA
GTCAGAAGCCCGAACCGATCACAAAGATCAAAAGTGAAAAGGAACCAAGAATTGATACGAAAATCAATGAATTGAATCGT
GTTTTAGGTGGAGGCGTCGTGCCGGGATCCCTTGTCCTGGTCGGAGGTGATCCGGGTATCGGAAAATCGACCCTCTTACT
TCAAGCCTCTGCAAAATTAGCCCAATCAGATCAGACGGTTTTATATATATCAGGTGAAGAATCGATCAAACAGACGAAAT
TGAGAGCCGAGAGGTTAGGGATCAATCATGATCGGTTGTATGTCCATGCAGAAACAGACATGGATTTGATTGAAAAAGCT
GTACAGGAAGTCAATCCATCACTCTTGATCATCGATTCGATTCAAACCGTATATCGATCGGAAATCGCTTCTGCACCCGG
CAGTGTTTCACAAGTACGGGAATGTACGTCCCAATTCATGCGGATCTCGAAAACGAATGGAATTGCCACTTTCATTGTCG
GACACGTTACGAAGGAAGGATCCATAGCAGGACCAAGACTCCTTGAACATATGGTAGATGCTGTCCTGTATTTTGAAGGA
GAGCGGCATCATACGTATCGAATTCTGCGGGCAGTGAAAAACCGTTTCGGATCGACAAATGAAATCGGTGTTTTCGAAAT
GAAGGAAGAAGGCCTTGATGAGGTGCAGAATCCTTCTGAAGTCTTTTTGGAAGAACGATCAAACGGGGCAGCTGGTTCTG
CTATCGTGGCATCGATGGAAGGCACACGTCCTGTTCTCGTTGAGATTCAAGCATTGGTTTCCCCTACGAGCTTCGGTAAC
CCGAGACGAATGGGAAATGGAATCGATCATAACCGGGTATCCTTATTAATGGCTGTCCTGGAAAAAAGGGTCGGGTTATT
GATGCAGAATCAGGATGCTTATGTGAATGTCGCAGGAGGGGTCCGCCTCGATGAACCAGCGATTGATTTAGCGATTGCTG
TCAGCATCGCATCAAGCTTCCGTGACCAACCGACAAATCCGACAGATGTCTATATCGGAGAAATCGGATTGACCGGCGAG
GTACGCAGAGTGTCTCGGGTTGAACAGCGTGTACATGAAGTCGCGAAACTAGGATTCAAACGGGTCATACTTCCAGAGAA
GAACCTCGGAGGCTGGTCAGCGCCGAAAGGAATTGAAATCGTAGGTGTATCATCTGTCCAAGAAACCCTACGTCACACAT
TAGGAGGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(344-428)

AAA domain (AAA_25)

(60-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

73.97

100

0.748
  radA Streptococcus pneumoniae Rx1

61.099

99.781

0.61
  radA Streptococcus pneumoniae D39

61.099

99.781

0.61
  radA Streptococcus pneumoniae R6

61.099

99.781

0.61
  radA Streptococcus pneumoniae TIGR4

61.099

99.781

0.61
  radA Streptococcus mitis NCTC 12261

61.099

99.781

0.61
  radA Streptococcus mitis SK321

60.879

99.781

0.607