Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MWM05_RS02065 Genome accession   NZ_AP025593
Coordinates   380112..382781 (-) Length   889 a.a.
NCBI ID   WP_244363123.1    Uniprot ID   -
Organism   Thermus brockianus strain SNM4-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 375112..387781
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWM05_RS02045 (TbrSNM41_03920) - 375955..376584 (-) 630 WP_071676690.1 histidine phosphatase family protein -
  MWM05_RS02050 (TbrSNM41_03930) purM 376581..377582 (-) 1002 WP_244363116.1 phosphoribosylformylglycinamidine cyclo-ligase -
  MWM05_RS02055 (TbrSNM41_03940) gatB 377588..378997 (-) 1410 WP_071676688.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  MWM05_RS02060 (TbrSNM41_03950) pilT 379015..380100 (-) 1086 WP_244363120.1 type IV pilus twitching motility protein PilT Machinery gene
  MWM05_RS02065 (TbrSNM41_03960) pilF 380112..382781 (-) 2670 WP_244363123.1 type IV pilus assembly ATPase PilB Machinery gene
  MWM05_RS02070 (TbrSNM41_03970) - 382778..383260 (-) 483 WP_244363125.1 YqeG family HAD IIIA-type phosphatase -
  MWM05_RS02075 (TbrSNM41_03980) pgeF 383296..384021 (-) 726 WP_244363128.1 peptidoglycan editing factor PgeF -
  MWM05_RS02080 (TbrSNM41_03990) - 384093..384497 (+) 405 WP_071676683.1 DUF4395 domain-containing protein -
  MWM05_RS02085 (TbrSNM41_04000) ribH 384484..384957 (-) 474 WP_071676682.1 6,7-dimethyl-8-ribityllumazine synthase -
  MWM05_RS02090 (TbrSNM41_04010) crcB 384978..385349 (+) 372 WP_071676681.1 fluoride efflux transporter CrcB -
  MWM05_RS02095 (TbrSNM41_04020) aroQ 385346..385783 (-) 438 WP_243093684.1 type II 3-dehydroquinate dehydratase -
  MWM05_RS02100 (TbrSNM41_04030) - 385813..386781 (+) 969 WP_243093685.1 LysM peptidoglycan-binding domain-containing M23 family metallopeptidase -

Sequence

Protein


Download         Length: 889 a.a.        Molecular weight: 97929.95 Da        Isoelectric Point: 5.2167

>NTDB_id=92928 MWM05_RS02065 WP_244363123.1 380112..382781(-) (pilF) [Thermus brockianus strain SNM4-1]
MSVLTIGDKRLGAILLDAGLLTDEELQLALEKHREVGGSLAEVIVESGLLSERRIAQAIEDHFGIPLVELHQLEIPPKVK
ALLPAEKAKELGAIPFALDEEAGVVRVAFVNPLDTLALEEVEDLTGLVVEPYQTTKSAFQYALAKNYPELGLPLPPPPTG
PSQGELRLGEFLLEKGLIDRETLEEALVEQERTGDLLGQILVRKGLSEEVLYRTLAEQKGLEFLPSTEGLTPDPAATALL
LRSDALRYSAVPVAIREGKVEVVLADPRHKQAVEELLGRPARFYLTLPKPWEALFHRAYPEKGRLGEVLVQEGRLSREHL
REALEVQKRLPKAKPLGEILVELGLARPEDVEEALKKQRQGGGRLEDTLVQSGKLKPEALAQAVAHQLGYPYIDPQENPP
DPGAALLLPEDLARRYGVFPHHLEGKTLVLLMKDPRNILALDDVRLALKRKGLGYEVTPAVATEAAIVKLIERFYGKEEL
GELAKELSKGYQAEEEALTTELDESAAQRFVKQVIREAYLQDASDIHIEPRQSDVLVRLRIDGTLRQYTTLSKGALGPVI
SVIKIMGGLNIAEKRLPQDGRVRYREGAIDADLRLSTLPTVYGEKAVMRLLKKAADIPEIEGLGFAPGVFERFQEVISKP
YGIFLITGPTGSGKSFTTFSILKRIATPDKNTQTIEDPVEYEIPGINQTQVNPQAGLTFARALRAFLRQDPDIIMVGEIR
DSETAKIATEAALTGHLVIATLHTNDAAQAITRLDEMGVELFNISAALIGVLSQRLVRRICEHCKVEVKPDPEVLRRLGI
PEEEMQGAKLYKGTGCERCGGTGYKGRYAIHELLVVDDEIRHAIVAGKSATEIKEIARKKGMKTLREDGVYKALQGITTL
EEVLARTIE

Nucleotide


Download         Length: 2670 bp        

>NTDB_id=92928 MWM05_RS02065 WP_244363123.1 380112..382781(-) (pilF) [Thermus brockianus strain SNM4-1]
ATGAGCGTGCTGACCATTGGCGACAAGCGGCTTGGGGCCATCCTTTTGGACGCCGGGCTCCTCACGGACGAGGAACTCCA
GCTGGCCCTGGAGAAGCACCGGGAGGTGGGGGGAAGCCTGGCGGAGGTCATCGTGGAATCGGGCCTCCTCTCGGAAAGGC
GCATCGCTCAGGCCATTGAGGACCACTTCGGCATCCCCCTGGTGGAACTCCACCAGCTGGAAATCCCCCCGAAGGTGAAG
GCCCTCTTGCCGGCGGAGAAGGCTAAGGAGCTGGGCGCCATCCCCTTTGCCCTGGACGAGGAGGCGGGGGTGGTGCGGGT
GGCCTTCGTGAACCCCTTGGACACCTTGGCCCTCGAGGAGGTGGAGGACCTCACGGGCCTGGTGGTCGAACCCTACCAAA
CCACCAAGAGCGCCTTCCAGTACGCCCTGGCCAAGAACTACCCCGAGCTCGGCCTACCCCTTCCGCCCCCACCCACGGGG
CCAAGCCAAGGGGAGCTCCGCTTAGGGGAGTTCCTCCTGGAAAAGGGCCTCATTGACCGGGAAACCTTGGAAGAGGCCTT
GGTGGAACAGGAGCGGACGGGGGACCTCTTAGGCCAGATCCTGGTGCGAAAGGGGCTTTCCGAGGAGGTCCTCTATCGGA
CCCTGGCCGAGCAAAAGGGGCTAGAATTCCTGCCCTCCACCGAGGGGCTAACCCCCGACCCCGCCGCCACCGCCCTCCTC
CTGCGCTCGGATGCCCTGCGCTATAGCGCCGTTCCCGTGGCCATCCGCGAGGGCAAAGTGGAGGTGGTCCTGGCCGACCC
CCGGCACAAGCAGGCGGTGGAGGAGCTTCTGGGCCGCCCGGCCCGTTTCTACCTCACCCTGCCCAAGCCCTGGGAAGCCC
TCTTCCACCGGGCCTACCCGGAAAAGGGGCGTTTGGGAGAGGTCTTGGTGCAGGAGGGCCGCCTGAGCCGGGAACACCTG
CGGGAAGCCTTGGAGGTGCAAAAGCGCCTCCCCAAAGCGAAGCCCTTGGGGGAGATCCTGGTGGAGCTTGGCCTGGCCCG
CCCCGAGGACGTGGAGGAGGCCCTCAAGAAGCAACGCCAAGGGGGAGGCCGCCTGGAAGACACCTTGGTCCAGTCGGGCA
AGCTCAAGCCCGAGGCCCTGGCCCAGGCGGTGGCCCACCAGCTGGGCTACCCCTATATTGACCCCCAGGAAAATCCTCCA
GACCCCGGCGCCGCCCTCCTCCTCCCCGAGGACCTAGCCCGGCGCTACGGCGTCTTCCCCCACCACCTGGAGGGGAAAAC
CTTGGTCCTTCTCATGAAGGACCCCCGGAACATCCTGGCCCTGGACGACGTGCGCCTGGCCTTGAAGCGGAAGGGCCTGG
GGTACGAGGTGACCCCCGCCGTGGCCACCGAGGCGGCCATCGTCAAGCTCATTGAGCGCTTCTACGGCAAGGAGGAGCTG
GGCGAGCTGGCCAAGGAGCTCTCCAAGGGGTACCAGGCGGAGGAAGAAGCCCTCACCACGGAGCTGGACGAAAGCGCCGC
CCAGCGCTTCGTCAAGCAGGTGATCCGGGAGGCCTACCTTCAGGACGCCTCCGACATTCACATAGAACCCCGCCAGTCGG
ACGTCCTGGTGCGCCTACGCATTGACGGTACCCTGCGCCAATACACCACCTTGTCTAAAGGGGCCTTGGGCCCGGTGATC
AGCGTCATCAAGATCATGGGCGGGCTCAACATCGCGGAAAAGCGCCTCCCCCAGGACGGCCGCGTCCGCTACCGGGAAGG
GGCCATTGACGCGGACCTCAGGCTTTCCACCCTCCCCACGGTCTACGGGGAAAAGGCGGTGATGCGCCTTTTGAAGAAGG
CGGCGGACATCCCCGAGATAGAGGGGCTGGGCTTCGCCCCCGGGGTCTTTGAGCGCTTCCAGGAAGTGATCTCCAAGCCC
TACGGCATCTTCCTCATCACCGGCCCCACGGGAAGCGGCAAGAGCTTCACCACCTTCTCCATCCTGAAGCGCATCGCCAC
CCCCGACAAGAACACCCAGACCATTGAAGACCCCGTGGAGTACGAGATCCCCGGCATCAACCAGACCCAGGTGAACCCCC
AAGCCGGGCTCACCTTCGCCCGGGCCTTGCGCGCCTTCCTGCGCCAGGACCCGGACATCATCATGGTGGGGGAGATCCGC
GACTCGGAAACCGCCAAGATCGCCACGGAGGCGGCCCTCACCGGCCACCTGGTCATCGCTACCCTGCACACCAACGACGC
AGCCCAGGCCATCACCCGCTTGGACGAGATGGGGGTGGAGCTTTTCAACATCTCCGCCGCCCTCATCGGCGTCCTTTCCC
AGCGCTTGGTGCGCAGGATCTGCGAGCACTGCAAGGTGGAGGTGAAGCCCGACCCCGAGGTCCTCCGCCGGCTCGGTATC
CCCGAGGAGGAGATGCAAGGAGCAAAGCTCTACAAGGGCACGGGGTGCGAGCGCTGCGGGGGCACGGGGTACAAGGGCCG
CTACGCTATCCACGAGCTGTTGGTGGTGGACGACGAGATCCGGCACGCCATCGTGGCGGGCAAGTCGGCCACGGAAATCA
AGGAGATCGCCCGCAAGAAGGGCATGAAAACCTTGCGGGAAGACGGCGTCTACAAGGCCCTCCAGGGAATTACCACCCTC
GAGGAGGTCCTGGCGCGTACCATTGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(513-779)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-148)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(369-479)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(201-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

86.839

100

0.868
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

56.292

100

0.564

Multiple sequence alignment