Detailed information NTG0928913

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: V1232_RS30720
• Genome accession: NZ_CP143357
• Coordinates: 6527192..6528340 (-)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain 2023CK-01621
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6522192..6533340

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V1232_RS30700 (V1232_30700)	-	6522930..6524114 (-)	1185	WP_003110017.1	cystathionine gamma-synthase	-
V1232_RS30705 (V1232_30705)	-	6524111..6525484 (-)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
V1232_RS30710 (V1232_30710)	-	6525727..6526131 (-)	405	WP_003084560.1	NINE protein	-
V1232_RS30715 (V1232_30715)	czcD	6526285..6527184 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
V1232_RS30720 (V1232_30720)	pilU	6527192..6528340 (-)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
V1232_RS30725 (V1232_30725)	pilT	6528518..6529552 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
V1232_RS30730 (V1232_30730)	-	6529767..6530459 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
V1232_RS30735 (V1232_30735)	proC	6530471..6531292 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
V1232_RS30740 (V1232_30740)	-	6531303..6531896 (+)	594	WP_003084544.1	YggT family protein	-
V1232_RS30745 (V1232_30745)	-	6531893..6532189 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=928913 V1232_RS30720 WP_003110016.1 6527192..6528340(-) (pilU) [Pseudomonas aeruginosa strain 2023CK-01621]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=928913 V1232_RS30720 WP_003110016.1 6527192..6528340(-) (pilU) [Pseudomonas aeruginosa strain 2023CK-01621]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382