Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   KAN5_RS16490 Genome accession   NZ_AP025589
Coordinates   3710824..3712320 (-) Length   498 a.a.
NCBI ID   WP_016710073.1    Uniprot ID   A0A5S3RL53
Organism   Pseudoalteromonas sp. KAN5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3705824..3717320
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KAN5_RS16460 (KAN5_32210) - 3706230..3706388 (+) 159 WP_016710088.1 YqaE/Pmp3 family membrane protein -
  KAN5_RS16465 (KAN5_32220) mgtE 3707255..3708613 (-) 1359 WP_016710078.1 magnesium transporter -
  KAN5_RS16470 (KAN5_32230) - 3708731..3709003 (-) 273 WP_016710077.1 HPr family phosphocarrier protein -
  KAN5_RS16475 (KAN5_32240) rapZ 3709031..3709873 (-) 843 WP_016710076.1 RNase adapter RapZ -
  KAN5_RS16480 (KAN5_32250) ptsN 3710051..3710503 (-) 453 WP_016710075.1 PTS IIA-like nitrogen regulatory protein PtsN -
  KAN5_RS16485 (KAN5_32260) hpf 3710508..3710795 (-) 288 WP_016710074.1 ribosome hibernation promoting factor -
  KAN5_RS16490 (KAN5_32270) rpoN/rpoN1 3710824..3712320 (-) 1497 WP_016710073.1 RNA polymerase factor sigma-54 Machinery gene
  KAN5_RS16495 (KAN5_32280) lptB 3712387..3713112 (-) 726 WP_016710072.1 LPS export ABC transporter ATP-binding protein -
  KAN5_RS16500 (KAN5_32290) lptA 3713109..3713642 (-) 534 WP_016710071.1 lipopolysaccharide transport periplasmic protein LptA -
  KAN5_RS16505 (KAN5_32300) lptC 3713632..3714186 (-) 555 WP_016710070.1 LPS export ABC transporter periplasmic protein LptC -
  KAN5_RS16510 (KAN5_32310) kdsC 3714183..3714734 (-) 552 WP_138555744.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  KAN5_RS16515 (KAN5_32320) - 3714734..3715705 (-) 972 WP_016710068.1 KpsF/GutQ family sugar-phosphate isomerase -
  KAN5_RS16520 (KAN5_32330) - 3715730..3716701 (-) 972 WP_138555743.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 56249.61 Da        Isoelectric Point: 4.4830

>NTDB_id=92889 KAN5_RS16490 WP_016710073.1 3710824..3712320(-) (rpoN/rpoN1) [Pseudoalteromonas sp. KAN5]
MRQSLQLRMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALDSNPLLELEEHEYSDDTAGKNEQKQDNDDLTVSASADDK
PSEYEQESSEALTKETLSDEMAMDVTWDEYMSAAPNSSAGPMPEDESIYQGASTETLHEYLMWQLQLTPFSPTDEAIAIA
IVEAIDDSGILTLSCEDIVESLNQGNNEEEIELDEVQAVLKRIQLFDPVGIAARSLQECLCIQLNQFDPATPWINETKMI
LTDHIDLLASRDYRTLMKKTKLKEDELKEVMTLIHSLNPKPADTIVREESEYVIPDVSVKKIKGRWVVELNPDSMPKIRV
NSQYAAMSRSVKSSTDSQFIRSHLQEAKWFIKSLESRNDTLLKVTNCIVKQQQGFFEHGPEAMRPMVLNDVAEMVEMHES
TISRVTTQKYMHTPRGIFELKYFFSSHVSTENGGECSSTAIRALIKKLISAENSAKPLSDSKIADILAEQGIKVARRTIA
KYRESLAIPPSNQRKSLI

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=92889 KAN5_RS16490 WP_016710073.1 3710824..3712320(-) (rpoN/rpoN1) [Pseudoalteromonas sp. KAN5]
ATGAGGCAATCACTCCAGCTGCGCATGGGCCAGCAACTTACAATGACACCGCAACTGCAACAAGCTATCCGCTTATTGCA
GCTCAGCACATTGGATCTGCAGCAAGAAATTCAAGAAGCGTTAGATAGCAATCCGTTACTAGAGCTAGAAGAGCATGAGT
ATTCAGACGACACGGCGGGCAAGAATGAGCAAAAGCAAGATAACGACGACTTAACCGTTAGCGCCTCAGCAGATGACAAG
CCCAGCGAATACGAACAAGAAAGCAGCGAAGCACTCACCAAAGAAACACTCAGTGATGAAATGGCGATGGATGTCACATG
GGATGAGTACATGAGCGCTGCACCAAATAGCTCAGCAGGCCCAATGCCAGAGGACGAATCTATATATCAAGGCGCCTCAA
CAGAAACCTTGCATGAATATTTAATGTGGCAGCTACAGCTAACTCCATTTAGCCCTACAGATGAAGCAATAGCCATTGCA
ATTGTTGAGGCCATTGATGACTCAGGTATTTTAACGCTTAGCTGTGAAGACATTGTAGAGAGCTTAAATCAAGGCAACAA
CGAAGAAGAAATCGAACTTGACGAAGTACAAGCGGTTTTAAAACGTATCCAATTATTTGACCCTGTAGGCATCGCGGCTC
GCAGTCTACAAGAGTGTTTATGTATTCAGCTAAATCAATTTGATCCGGCAACCCCTTGGATTAATGAGACAAAAATGATT
TTAACTGATCATATCGATTTATTAGCTAGTCGCGACTATCGCACTTTGATGAAAAAGACTAAGCTTAAGGAAGATGAACT
AAAAGAAGTGATGACTCTGATCCACAGCCTAAATCCAAAGCCTGCGGATACCATAGTACGAGAAGAGTCAGAATACGTTA
TTCCTGACGTATCAGTGAAAAAAATTAAAGGCCGCTGGGTGGTTGAACTTAATCCAGACAGCATGCCAAAAATCAGAGTG
AACAGCCAATATGCAGCTATGTCGCGCTCAGTAAAGTCGAGCACTGATAGTCAGTTTATTCGTTCGCATCTGCAAGAAGC
TAAATGGTTTATAAAAAGCCTAGAAAGTCGCAATGACACATTGCTCAAAGTAACCAATTGCATCGTAAAACAGCAGCAAG
GTTTCTTTGAACATGGCCCTGAAGCCATGCGACCTATGGTACTCAATGACGTTGCTGAAATGGTAGAGATGCATGAGTCA
ACAATTTCTCGTGTAACTACCCAAAAATACATGCATACCCCGCGTGGTATTTTTGAGTTAAAGTATTTTTTCTCAAGCCA
TGTTAGCACTGAAAATGGCGGCGAATGCTCATCGACAGCTATTCGCGCACTAATCAAAAAACTGATTAGCGCAGAAAACT
CAGCGAAACCATTGAGTGATAGTAAAATTGCAGATATATTGGCAGAGCAAGGTATCAAAGTAGCTAGACGGACTATTGCA
AAATACCGCGAGTCACTGGCTATTCCACCGTCAAATCAAAGAAAGAGTTTGATTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(132-324)

Sigma-54, DNA binding domain (Sigma54_DBD)

(338-495)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S3RL53

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.332

100

0.482

Multiple sequence alignment