Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   SA496_RS02065 Genome accession   NZ_CP143312
Coordinates   409988..411352 (+) Length   454 a.a.
NCBI ID   WP_120653022.1    Uniprot ID   -
Organism   Pseudomonas sp. JS3066     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 404988..416352
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SA496_RS02040 (SA496_02040) - 406276..406752 (+) 477 WP_153921589.1 DUF934 domain-containing protein -
  SA496_RS02045 (SA496_02045) - 407031..408593 (+) 1563 WP_120653026.1 TerC family protein -
  SA496_RS02050 (SA496_02050) mscL 408715..409131 (-) 417 WP_120653025.1 large-conductance mechanosensitive channel protein MscL -
  SA496_RS02055 (SA496_02055) - 409341..409586 (+) 246 WP_120653024.1 YdcH family protein -
  SA496_RS02060 (SA496_02060) - 409666..409944 (+) 279 WP_120653023.1 hypothetical protein -
  SA496_RS02065 (SA496_02065) radA 409988..411352 (+) 1365 WP_120653022.1 DNA repair protein RadA Machinery gene
  SA496_RS02070 (SA496_02070) - 411634..412002 (-) 369 WP_330114974.1 PilZ domain-containing protein -
  SA496_RS02075 (SA496_02075) - 412055..414220 (-) 2166 WP_330114975.1 hypothetical protein -
  SA496_RS02080 (SA496_02080) - 414207..415388 (-) 1182 WP_330115612.1 DUF3142 domain-containing protein -
  SA496_RS02085 (SA496_02085) - 415560..416066 (+) 507 WP_120653019.1 DUF3015 domain-containing protein -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 48404.85 Da        Isoelectric Point: 6.8310

>NTDB_id=928632 SA496_RS02065 WP_120653022.1 409988..411352(+) (radA) [Pseudomonas sp. JS3066]
MAKAKRMYGCTECGATFPKWAGQCADCGAWNTLVETVLEGAAATSGGRGGWAGQQAQIKTLAEVSVEEMPRFSTASTELD
RVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIATRLPALYVTGEESQQQVAMRARRLGLPEDKLKVMTETCIESIIAT
ARVEQPKVMVIDSIQTIFTEQLQSAPGGVAQVRESAALLVRFAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFEG
ESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQESVPGSVVMATWEGSRPMLVEVQALVDTSHLAN
PRRVTLGLDQNRLAMLLAVLHRHGGIPTYDQDVFLNVVGGVKVLETASDLALIAAIISSLRNRPLAHDVLVFGEVGLSGE
VRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEDPPGLTVVAVTRLEQALDALFE

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=928632 SA496_RS02065 WP_120653022.1 409988..411352(+) (radA) [Pseudomonas sp. JS3066]
ATGGCCAAGGCCAAGCGCATGTACGGCTGCACCGAGTGCGGCGCTACCTTCCCCAAATGGGCCGGCCAGTGTGCTGACTG
CGGGGCCTGGAACACCCTGGTGGAAACCGTGCTGGAGGGCGCCGCCGCTACCTCCGGGGGGCGCGGCGGCTGGGCCGGCC
AGCAGGCGCAGATCAAGACCCTGGCCGAGGTCAGCGTTGAAGAAATGCCGCGTTTTTCCACCGCTTCCACCGAGCTTGAT
CGGGTGCTCGGTGGTGGCTTGGTGGACGGGTCGGTGGTTCTGATCGGCGGCGATCCGGGCATCGGCAAATCCACCATCCT
CCTGCAAACCCTCTGCAACATCGCCACGCGTCTGCCCGCGCTCTATGTCACCGGTGAGGAGTCCCAGCAACAGGTCGCCA
TGCGTGCCCGCCGCCTGGGCTTGCCGGAAGACAAGCTGAAGGTCATGACCGAGACCTGCATCGAAAGCATCATCGCCACC
GCGCGGGTCGAGCAGCCCAAGGTGATGGTGATCGATTCCATCCAGACTATCTTCACCGAGCAATTGCAGTCCGCCCCAGG
CGGCGTGGCCCAGGTCCGCGAGAGTGCGGCACTGCTGGTGCGTTTCGCCAAGCAGAGTGGCACGGCGATCTTCCTGGTCG
GCCATGTGACCAAGGAAGGCGCCCTGGCTGGCCCGCGCGTGCTGGAGCACATGGTGGACACCGTGCTCTATTTCGAGGGC
GAGTCCGACGGCCGACTGCGCCTGTTGCGGGCGGTGAAGAATCGTTTCGGTGCGGTCAACGAGCTGGGCGTGTTCGGCAT
GACGGACAAAGGCCTGAAGGAGGTTTCCAACCCCTCGGCGATCTTCCTCACACGCGCTCAGGAGTCCGTGCCCGGCAGCG
TGGTAATGGCGACCTGGGAAGGCTCGCGGCCCATGCTGGTGGAGGTTCAGGCGCTGGTCGACACCAGCCACCTGGCCAAC
CCGCGACGGGTAACCCTGGGGCTGGACCAGAACCGCCTGGCCATGTTGCTGGCGGTATTGCACCGGCACGGCGGCATCCC
GACCTACGATCAGGATGTGTTCCTCAACGTGGTTGGCGGTGTGAAGGTACTTGAGACCGCTTCCGACCTTGCGCTGATCG
CCGCCATCATCTCCAGTCTGCGCAACCGCCCCCTGGCCCATGATGTGCTGGTGTTCGGTGAAGTGGGGTTGTCCGGTGAG
GTACGTCCGGTGCCCAGCGGCCAGGAGCGCCTGAAGGAAGCCGCCAAGCATGGTTTCAAGCGGGCGATCGTGCCCAAGGG
CAACGCCCCCAAGGAAGATCCGCCGGGCCTCACCGTGGTCGCGGTGACGCGCCTGGAGCAGGCGTTGGATGCGCTGTTCG
AGTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(362-428)

AAA domain (AAA_25)

(72-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.451

99.559

0.482
  radA Streptococcus pneumoniae Rx1

46.288

100

0.467
  radA Streptococcus pneumoniae R6

46.288

100

0.467
  radA Streptococcus pneumoniae TIGR4

46.288

100

0.467
  radA Streptococcus pneumoniae D39

46.288

100

0.467
  radA Streptococcus mitis SK321

46.476

100

0.465
  radA Streptococcus mitis NCTC 12261

46.256

100

0.463