Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   VSX76_RS06400 Genome accession   NZ_CP143311
Coordinates   934066..935136 (-) Length   356 a.a.
NCBI ID   WP_330110977.1    Uniprot ID   -
Organism   Methylophaga thalassica strain IOM01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 929066..940136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VSX76_RS06365 (VSX76_06360) arsC 929162..929512 (-) 351 WP_273182333.1 arsenate reductase (glutaredoxin) -
  VSX76_RS06370 (VSX76_06365) lapB 929509..930690 (-) 1182 WP_330111253.1 lipopolysaccharide assembly protein LapB -
  VSX76_RS06375 (VSX76_06370) - 930695..930988 (-) 294 WP_007145801.1 lipopolysaccharide assembly LapA domain-containing protein -
  VSX76_RS06380 (VSX76_06375) fdxA 931154..931477 (+) 324 WP_007145800.1 ferredoxin FdxA -
  VSX76_RS06385 (VSX76_06380) rpoH 931530..932390 (-) 861 WP_007145799.1 RNA polymerase sigma factor RpoH -
  VSX76_RS06390 (VSX76_06385) ftsX 932491..933417 (-) 927 WP_330110976.1 permease-like cell division protein FtsX -
  VSX76_RS06395 (VSX76_06390) ftsE 933419..934069 (-) 651 WP_007145797.1 cell division ATP-binding protein FtsE -
  VSX76_RS06400 (VSX76_06395) pilA 934066..935136 (-) 1071 WP_330110977.1 signal recognition particle-docking protein FtsY Machinery gene
  VSX76_RS06405 (VSX76_06400) - 935202..936569 (+) 1368 WP_330110978.1 pitrilysin family protein -
  VSX76_RS06410 (VSX76_06405) - 936559..937863 (+) 1305 WP_330110979.1 pitrilysin family protein -
  VSX76_RS06415 (VSX76_06410) rsmD 937847..938443 (+) 597 WP_330110980.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  VSX76_RS06420 (VSX76_06415) coaD 938440..938928 (+) 489 WP_007145792.1 pantetheine-phosphate adenylyltransferase -
  VSX76_RS06425 (VSX76_06420) - 938968..939222 (+) 255 WP_007145791.1 YfhL family 4Fe-4S dicluster ferredoxin -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 38625.07 Da        Isoelectric Point: 4.9461

>NTDB_id=928612 VSX76_RS06400 WP_330110977.1 934066..935136(-) (pilA) [Methylophaga thalassica strain IOM01]
MFSFLKRKSKTPASDASIAEDNTVDNSQELSPADEPAEEKTDNAPQPGFFSRLKQGLSRTSSKLTEGFASLILGRKNIDD
DLLEELETQLLTADLGVEATTRIITDLTQRVARKQLNDVEALFAAMREDMISILEPSSQPLEIPEKTDGPYVILMVGING
VGKTTTIGKLAKQFQQQGKKVMLAAGDTFRAAAVEQLQVWGERNNIPVIAQKTGADSASVIFDGLQAAKARNMDILIADT
AGRLHTQSNLMEELKKVKRVMAKVDDTAPHEVMLVLDAGTGQNALQQAQQFQQSVGVSGITLTKLDGTAKGGIIFAIANK
TKLPIRYIGVGEKIDDLRPFNATDFVDALLGKEEIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=928612 VSX76_RS06400 WP_330110977.1 934066..935136(-) (pilA) [Methylophaga thalassica strain IOM01]
ATGTTTAGTTTCCTTAAAAGAAAGTCAAAAACACCCGCGTCAGACGCCTCTATTGCTGAAGATAACACTGTTGATAATTC
GCAAGAATTATCCCCTGCTGATGAACCCGCAGAGGAAAAGACAGATAACGCACCGCAACCCGGTTTTTTTAGTCGTCTGA
AACAAGGCTTGAGCCGAACCAGTAGTAAACTTACTGAAGGTTTCGCCAGCTTAATTTTAGGTCGTAAAAACATTGATGAT
GACCTGCTGGAAGAGCTTGAAACTCAACTTCTTACAGCCGATCTTGGTGTTGAAGCAACGACACGAATTATTACTGATCT
GACTCAGCGCGTGGCCCGCAAACAATTAAACGACGTAGAAGCCTTATTTGCCGCTATGCGTGAGGATATGATTTCTATCC
TTGAACCGAGTAGTCAACCACTGGAAATACCAGAAAAAACTGACGGACCCTATGTGATTCTGATGGTAGGAATAAACGGT
GTGGGCAAAACCACTACCATCGGCAAACTCGCTAAGCAATTCCAACAGCAAGGTAAAAAAGTCATGCTGGCAGCTGGTGA
TACCTTCCGTGCAGCAGCGGTAGAACAGTTACAAGTATGGGGTGAGCGTAACAATATCCCGGTTATCGCACAAAAGACCG
GCGCTGACTCGGCTTCTGTCATTTTTGATGGATTACAAGCCGCTAAAGCCAGAAATATGGATATTCTGATTGCTGACACC
GCTGGCCGCCTGCATACACAGTCCAATTTAATGGAAGAGTTAAAAAAAGTGAAACGTGTCATGGCTAAAGTGGATGACAC
AGCACCTCATGAGGTCATGCTCGTGCTGGATGCAGGTACTGGACAAAATGCACTTCAGCAAGCACAGCAATTTCAACAAT
CTGTTGGTGTTTCAGGCATTACATTGACCAAATTAGATGGCACAGCCAAAGGCGGTATTATTTTCGCCATTGCCAATAAA
ACAAAATTGCCTATCCGTTATATTGGTGTGGGTGAAAAAATCGATGATCTACGTCCATTTAATGCCACTGATTTTGTTGA
TGCTTTGTTGGGAAAAGAAGAGATTAAATGA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(151-351)

SRP54-type protein, helical bundle domain (SRP54_N)

(64-128)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

57.096

85.112

0.486