Detailed information NTG0927919

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: BCC0191_RS14455
• Genome accession: NZ_CP142981
• Coordinates: 3132474..3133634 (+)
• Length: 386 a.a.
• NCBI ID: WP_006753287.1
• Uniprot ID: B1FJC3
• Organism: Burkholderia ambifaria strain BCC0191
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3127474..3138634

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BCC0191_RS14425 (BCC0191_002770)	-	3128111..3128719 (+)	609	WP_175672455.1	50S ribosomal protein L25/general stress protein Ctc	-
BCC0191_RS14430 (BCC0191_002771)	pth	3128838..3129437 (+)	600	WP_006753283.1	aminoacyl-tRNA hydrolase	-
BCC0191_RS14435 (BCC0191_002772)	hisC	3129563..3130630 (+)	1068	WP_175672456.1	histidinol-phosphate transaminase	-
BCC0191_RS14440 (BCC0191_002773)	-	3130724..3130990 (-)	267	WP_006753285.1	YfhL family 4Fe-4S dicluster ferredoxin	-
BCC0191_RS14445 (BCC0191_002774)	coaD	3131050..3131547 (-)	498	WP_006398238.1	pantetheine-phosphate adenylyltransferase	-
BCC0191_RS14450 (BCC0191_002775)	rsmD	3131659..3132273 (-)	615	WP_175672457.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
BCC0191_RS14455 (BCC0191_002776)	pilA	3132474..3133634 (+)	1161	WP_006753287.1	signal recognition particle-docking protein FtsY	Machinery gene
BCC0191_RS14460 (BCC0191_002778)	maiA	3133733..3134377 (-)	645	WP_006753289.1	maleylacetoacetate isomerase	-
BCC0191_RS14465 (BCC0191_002779)	ybiB	3134567..3135541 (+)	975	WP_006753290.1	DNA-binding protein YbiB	-
BCC0191_RS14470 (BCC0191_002780)	leuA	3135934..3137580 (+)	1647	WP_175672458.1	2-isopropylmalate synthase	-

Sequence

Protein

• Length: 386 a.a.
• Molecular weight: 41132.05 Da
• Isoelectric Point: 4.8762

>NTDB_id=927919 BCC0191_RS14455 WP_006753287.1 3132474..3133634(+) (pilA) [Burkholderia ambifaria strain BCC0191]
MFSFFKRFKKTQESDSTESQSADALQPDEPSDAPAPDARQAIEAPPAPAQPRPAAPAVVMTVTPANDGSDDVVEAVEIVP
PPTQDASAKKSWLVRLKTGLAKTGSSITGVFVNTKIDEDLYEELEAALLMSDAGVDATEYLLGALREKVKTGRLTDPQQV
KSALHDLLVELLKPLEKSLMLGRAQPLVMMITGVNGAGKTTSIGKLAKHLQSFDQSVLLAAGDTFRAAAREQLAVWGERN
NVTVVQQESGDPAAVIFDAVSAARARKIDVMMADTAGRLPTQLHLMEELKKVKRVISKAHDGAPHEVLLVIDANTGQNAL
TQVKAFDDALGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVGEKVEDLQPFSAVEFADALLD

Nucleotide

• Length: 1161 bp

>NTDB_id=927919 BCC0191_RS14455 WP_006753287.1 3132474..3133634(+) (pilA) [Burkholderia ambifaria strain BCC0191]
ATGTTCAGCTTCTTCAAACGATTCAAGAAAACGCAGGAGTCCGACTCCACGGAATCGCAATCGGCCGACGCGCTGCAACC
GGACGAGCCGTCCGACGCGCCCGCCCCCGATGCCCGTCAGGCGATCGAAGCACCGCCAGCGCCCGCGCAACCGCGACCGG
CCGCGCCGGCCGTCGTGATGACGGTCACGCCGGCCAACGACGGCAGCGACGACGTCGTCGAAGCGGTCGAGATCGTGCCG
CCGCCGACGCAGGACGCCTCGGCGAAGAAATCGTGGCTCGTGCGCCTGAAAACGGGGCTCGCGAAGACGGGTTCGAGCAT
CACCGGCGTGTTCGTCAACACGAAGATCGACGAGGATCTGTACGAAGAACTCGAAGCCGCGCTGCTGATGTCCGACGCCG
GCGTCGATGCAACCGAGTACCTGCTCGGTGCGCTGCGCGAGAAAGTGAAAACGGGCCGGCTGACCGATCCGCAGCAGGTG
AAGAGCGCGCTGCACGACCTGCTCGTCGAACTGCTGAAGCCACTCGAGAAATCGTTGATGCTCGGCCGCGCGCAGCCGCT
CGTGATGATGATCACGGGCGTGAACGGCGCGGGCAAGACCACCAGCATCGGCAAGCTCGCGAAGCATCTGCAGAGCTTCG
ACCAGTCGGTACTGCTGGCCGCGGGCGACACGTTCCGCGCGGCCGCACGCGAACAGCTGGCGGTCTGGGGCGAACGCAAC
AACGTGACGGTCGTCCAGCAGGAAAGCGGCGACCCGGCCGCGGTGATCTTCGACGCGGTCAGCGCCGCGCGCGCGCGCAA
GATCGACGTGATGATGGCCGACACGGCCGGCCGCCTGCCGACGCAGCTGCACCTGATGGAAGAGCTGAAGAAGGTGAAGC
GCGTGATCTCGAAGGCGCACGACGGCGCACCGCACGAGGTGCTGCTCGTGATCGACGCGAACACCGGCCAGAACGCGCTC
ACGCAGGTGAAGGCCTTCGACGACGCGCTCGGCCTCACGGGCCTGATCGTCACGAAGCTCGACGGCACCGCCAAGGGCGG
GATCCTCGCCGCGATCGCGCGGCAGCGCCCGGTGCCTGTCTACTTCATCGGCGTCGGCGAGAAGGTCGAGGACCTGCAGC
CGTTCAGCGCCGTGGAATTCGCGGACGCGCTACTCGACTGA

Domains

Predicted by InterproScan.

• SRP54-type protein, helical bundle domain (SRP54_N) (104-166)

• SRP54-type protein, GTPase domain (SRP54) (187-385)

3D structure

• AlphaFold DB: B1FJC3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	52.983	100	0.575