Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   VXM69_RS17135 Genome accession   NZ_CP142886
Coordinates   692611..694116 (+) Length   501 a.a.
NCBI ID   WP_034189274.1    Uniprot ID   -
Organism   Burkholderia sp. Ed8     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 687611..699116
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VXM69_RS17110 (VXM69_17090) kdsD 688843..689826 (+) 984 WP_034189278.1 arabinose 5-phosphate isomerase KdsD -
  VXM69_RS17115 (VXM69_17095) - 689826..690362 (+) 537 WP_367189947.1 KdsC family phosphatase -
  VXM69_RS17120 (VXM69_17100) lptC 690366..690980 (+) 615 WP_034189276.1 LPS export ABC transporter periplasmic protein LptC -
  VXM69_RS17125 (VXM69_17105) lptA 691001..691666 (+) 666 WP_006483139.1 lipopolysaccharide transport periplasmic protein LptA -
  VXM69_RS17130 (VXM69_17110) lptB 691663..692439 (+) 777 WP_034189275.1 LPS export ABC transporter ATP-binding protein -
  VXM69_RS17135 (VXM69_17115) rpoN/rpoN1 692611..694116 (+) 1506 WP_034189274.1 RNA polymerase factor sigma-54 Machinery gene
  VXM69_RS17140 (VXM69_17120) raiA 694275..694634 (+) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  VXM69_RS17145 (VXM69_17125) - 694829..695341 (+) 513 WP_049034209.1 PTS sugar transporter subunit IIA -
  VXM69_RS17150 (VXM69_17130) hprK 695471..696439 (+) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  VXM69_RS17155 (VXM69_17135) rapZ 696515..697423 (+) 909 WP_034189273.1 RNase adapter RapZ -
  VXM69_RS17160 (VXM69_17140) - 697451..698086 (+) 636 WP_034189272.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54721.84 Da        Isoelectric Point: 4.8711

>NTDB_id=927461 VXM69_RS17135 WP_034189274.1 692611..694116(+) (rpoN/rpoN1) [Burkholderia sp. Ed8]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAS
ANGSSTSSDRAERDEPAGADDYDGYGSGDGNDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPPGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=927461 VXM69_RS17135 WP_034189274.1 692611..694116(+) (rpoN/rpoN1) [Burkholderia sp. Ed8]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACACCTGGCGCTGACGCCGCAGCTGCAGCAGTCGATCCGGCTCCTGCA
GTTGTCCACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGTGTCGCGGCGGACGGATCGCTGATCGCGCAGACGCCGCCCGCGCAGAACACCGAGCCGGCCTCG
GCAAACGGCAGCAGCACGTCCAGCGATCGCGCGGAGCGCGACGAGCCGGCCGGTGCCGACGACTACGACGGTTACGGTTC
CGGCGACGGCAACGACGGCCCGCAATGGAATCTCGACGAATTCGGCCGCGCGAGCGGCGCGTCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAGTCGCTCGACGACGACGGCTACCTGACCGCGACGCTCGACGAGGTGCT
CGCCGACCTGCCGCCCGAGCTGGAGATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGC
CCGGGGTCGGCGCACGCTCGGCGTCCGAATGCCTGAAGCTGCAGCTGCTGCGCCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCACCTCGAACTGCTCGCGGCCCGCGATTTCACACGGTTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCATGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGGTGGCTCGCGGAACTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGTTCGCTGAAGCAGCA
GCTCCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGTTTCGACACGATCCTGCGAGTCGCGCAGGCTATTGTCG
AGCGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTGGAATTTAAGTACTT
CTTCGGATCTCACGTTTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.182

100

0.689