Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   VJ374_RS00540 Genome accession   NZ_CP142850
Coordinates   96813..98189 (+) Length   458 a.a.
NCBI ID   WP_029343076.1    Uniprot ID   -
Organism   Exiguobacterium sp. 9-2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 91813..103189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VJ374_RS00520 (VJ374_00520) - 92315..92770 (+) 456 WP_023466578.1 CtsR family transcriptional regulator -
  VJ374_RS00525 (VJ374_00525) - 92796..93224 (+) 429 WP_029343073.1 hypothetical protein -
  VJ374_RS00530 (VJ374_00530) - 93217..94290 (+) 1074 WP_329469725.1 protein arginine kinase -
  VJ374_RS00535 (VJ374_00535) clpC 94290..96737 (+) 2448 WP_035413120.1 ATP-dependent protease ATP-binding subunit ClpC -
  VJ374_RS00540 (VJ374_00540) radA 96813..98189 (+) 1377 WP_029343076.1 DNA repair protein RadA Machinery gene
  VJ374_RS00545 (VJ374_00545) - 98317..99402 (+) 1086 WP_035413125.1 PIN/TRAM domain-containing protein -
  VJ374_RS00550 (VJ374_00550) ispD 99534..100217 (+) 684 WP_329469727.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  VJ374_RS00555 (VJ374_00555) ispF 100220..100702 (+) 483 WP_029343079.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  VJ374_RS00560 (VJ374_00560) gltX 100746..102194 (+) 1449 WP_290774586.1 glutamate--tRNA ligase -
  VJ374_RS00565 (VJ374_00565) epsC 102426..103145 (+) 720 WP_313489801.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50039.64 Da        Isoelectric Point: 6.6525

>NTDB_id=927352 VJ374_RS00540 WP_029343076.1 96813..98189(+) (radA) [Exiguobacterium sp. 9-2]
MAKLKTKFVCQSCGTESPKWMGRCSGCGEWNTMVEEVVEEKKGRRGAAFVHTTTKQLKPERLANIVSQEESRVFTGSGEF
DRVLGGGIVPGSMVLVGGDPGIGKSTILLQTSARLAQRGEKVLYISGEESLKQTKLRAERLGLPTQDLFVLSETDMNMIE
RVVDEEQPRFLIIDSIQTVYIDEIQSAPGSVTQVRECTAMLMKIAKSRGIAIFIVGHVTKQGSIAGPRLLEHMVDAVLYF
EGERHHTFRILRAVKNRFGSTNEIGIFEMRESGLEEVLNPSEIFLEERTSGVSGSTIVASMEGTRTVLVELQALISPTSF
GNPRRMATGIDQNKVALLMAVLEKRSGLLLQTQDAYLKAAGGVKLDEPAIDLAVCVAIASSFRDKPTRPTDVVIGEVGLT
GEVRRVSRIEQRVAEAAKLGFTRAIIPKNNLGGWTYPDGITVVGVESVDEALRETIPF

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=927352 VJ374_RS00540 WP_029343076.1 96813..98189(+) (radA) [Exiguobacterium sp. 9-2]
TTGGCTAAGTTAAAAACAAAATTCGTTTGCCAAAGCTGTGGAACTGAATCACCGAAATGGATGGGACGTTGTTCTGGATG
CGGAGAATGGAACACGATGGTCGAAGAAGTCGTCGAAGAAAAAAAAGGACGGCGCGGTGCCGCTTTTGTTCATACGACGA
CAAAACAATTAAAGCCTGAACGCCTCGCGAATATCGTCTCGCAAGAAGAGAGTCGTGTCTTTACCGGAAGTGGTGAATTC
GATCGTGTCCTCGGAGGCGGCATCGTTCCAGGTTCGATGGTGCTTGTTGGAGGAGACCCGGGGATCGGGAAGTCGACGAT
CTTACTGCAGACGAGTGCACGGCTTGCCCAACGTGGTGAAAAAGTGCTCTACATCTCAGGGGAAGAATCGCTTAAACAAA
CCAAATTGCGCGCGGAACGGCTTGGATTGCCGACACAGGATTTGTTCGTCCTTAGTGAAACGGACATGAACATGATTGAA
CGTGTCGTTGATGAAGAACAGCCACGGTTTTTAATCATCGACTCGATTCAAACCGTCTACATTGATGAAATTCAGTCCGC
ACCCGGGAGTGTGACACAGGTACGTGAATGTACGGCGATGCTGATGAAAATTGCAAAGAGTCGTGGTATTGCAATCTTTA
TCGTTGGTCATGTTACGAAACAGGGGTCGATTGCTGGACCACGTTTACTTGAGCACATGGTGGATGCTGTCTTGTATTTC
GAAGGCGAACGTCACCATACGTTTCGGATTCTGCGTGCTGTTAAAAACCGATTTGGTTCAACGAATGAGATCGGAATTTT
TGAAATGCGGGAGTCTGGTCTTGAAGAGGTCTTAAATCCGTCAGAAATTTTCCTCGAAGAGCGGACATCCGGCGTATCCG
GTTCAACGATCGTTGCTTCAATGGAAGGGACACGGACCGTCCTCGTCGAACTGCAAGCATTGATTTCACCGACGTCATTT
GGGAATCCTAGACGGATGGCGACCGGAATCGATCAAAATAAAGTAGCGTTATTGATGGCCGTTCTTGAGAAACGATCGGG
TCTTCTCTTACAAACACAAGATGCCTATCTCAAGGCAGCGGGTGGTGTGAAGCTCGATGAACCAGCGATCGACTTAGCAG
TCTGTGTTGCCATTGCCTCTAGTTTCCGTGACAAGCCGACGCGTCCGACGGATGTCGTGATTGGTGAGGTCGGGTTGACG
GGAGAAGTCCGTCGTGTTTCACGGATTGAGCAACGTGTAGCAGAAGCAGCCAAACTCGGTTTTACACGAGCGATCATTCC
GAAAAATAACTTAGGAGGCTGGACGTATCCGGACGGGATTACGGTCGTCGGTGTCGAAAGCGTGGACGAAGCATTACGCG
AAACAATTCCGTTTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(83-222)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

72.588

99.563

0.723
  radA Streptococcus pneumoniae Rx1

62.775

99.127

0.622
  radA Streptococcus pneumoniae D39

62.775

99.127

0.622
  radA Streptococcus pneumoniae R6

62.775

99.127

0.622
  radA Streptococcus pneumoniae TIGR4

62.775

99.127

0.622
  radA Streptococcus mitis NCTC 12261

62.775

99.127

0.622
  radA Streptococcus mitis SK321

62.775

99.127

0.622