Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   VJ143_RS18800 Genome accession   NZ_CP142740
Coordinates   4038536..4039339 (-) Length   267 a.a.
NCBI ID   WP_004725881.1    Uniprot ID   A0A6A1RK51
Organism   Acinetobacter guillouiae strain 13-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4033536..4044339
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VJ143_RS18780 (VJ143_18790) - 4034596..4035297 (-) 702 WP_004816992.1 YfgM family protein -
  VJ143_RS18785 (VJ143_18795) hisS 4035328..4036623 (-) 1296 WP_056512303.1 histidine--tRNA ligase -
  VJ143_RS18790 (VJ143_18800) ispG 4036620..4037735 (-) 1116 WP_004725885.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  VJ143_RS18795 (VJ143_18805) - 4037757..4038545 (-) 789 WP_004725882.1 helix-turn-helix domain-containing protein -
  VJ143_RS18800 (VJ143_18810) pilF 4038536..4039339 (-) 804 WP_004725881.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  VJ143_RS18805 (VJ143_18815) rlmN 4039405..4040649 (-) 1245 WP_004725880.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  VJ143_RS18810 (VJ143_18820) ndk 4040828..4041259 (-) 432 WP_004725878.1 nucleoside-diphosphate kinase -
  VJ143_RS18815 (VJ143_18825) iscX 4041403..4041603 (-) 201 WP_004725877.1 Fe-S cluster assembly protein IscX -
  VJ143_RS18820 (VJ143_18830) - 4041822..4042373 (-) 552 WP_004725875.1 phosphatase PAP2 family protein -
  VJ143_RS18825 (VJ143_18835) - 4042405..4043691 (-) 1287 WP_327857778.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 267 a.a.        Molecular weight: 29946.10 Da        Isoelectric Point: 9.2123

>NTDB_id=926653 VJ143_RS18800 WP_004725881.1 4038536..4039339(-) (pilF) [Acinetobacter guillouiae strain 13-3]
MYQKAVVILAIGLSALVVQGCQTTNSDTIIKKDPEKAVKVRTQLAAEYIRTGDLDAAKRALDQALETDSRDSAANMMMGV
LLQQEGSKISMEKADGYFKRAISSDSKNAQARNNYGTYLFVTERYNDAIEQLSIAGAALGYDQRYKALENLGRVYLKVGD
VANAEKAFKQALQVNRDSIISLVELSEIFYLQQKSAQASQLYEQYVRLVGQDKQGARALWIGLRIARANNDQMGMQVLVN
QLRALFPDSKEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 804 bp        

>NTDB_id=926653 VJ143_RS18800 WP_004725881.1 4038536..4039339(-) (pilF) [Acinetobacter guillouiae strain 13-3]
ATGTATCAAAAAGCAGTTGTAATATTGGCAATTGGTTTATCTGCATTGGTCGTACAGGGTTGTCAAACGACAAATTCAGA
CACAATAATAAAAAAAGATCCTGAAAAAGCCGTTAAAGTACGTACTCAGCTTGCTGCTGAATATATTCGTACAGGTGATT
TAGATGCTGCAAAACGCGCACTAGACCAAGCTTTAGAAACTGATTCACGCGATTCTGCGGCAAATATGATGATGGGTGTC
TTACTGCAACAAGAAGGCAGTAAGATCAGTATGGAAAAAGCAGACGGCTATTTTAAGCGTGCAATTTCCAGTGACTCAAA
AAATGCACAAGCTCGTAATAATTATGGAACATATTTATTTGTTACTGAGCGCTATAATGACGCGATTGAACAGTTGAGTA
TAGCTGGTGCTGCGCTTGGCTATGATCAGCGTTACAAGGCTTTAGAAAATTTAGGTCGGGTTTACTTAAAAGTGGGTGAT
GTGGCAAATGCTGAAAAAGCATTCAAACAAGCTTTACAAGTCAATCGCGATTCGATTATTTCTCTGGTAGAGTTATCAGA
AATTTTCTATTTGCAACAAAAAAGTGCGCAAGCAAGTCAACTCTATGAGCAATATGTTCGTTTAGTAGGTCAGGACAAAC
AGGGTGCGAGAGCGCTTTGGATTGGTTTACGTATTGCACGTGCCAATAATGATCAGATGGGAATGCAAGTGTTGGTCAAC
CAACTGCGTGCATTGTTCCCGGACAGTAAAGAATATCAACGTTATCTGCAATTACAGTACAGTACTGAGGCCGTATGGAA
GTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_2)

(146-177)

Tetratricopeptide repeat (TPR_8)

(40-69)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6A1RK51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

74.717

99.251

0.742
  pilF Acinetobacter baylyi ADP1

67.843

95.506

0.648