Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   VSY18_RS20380 Genome accession   NZ_CP142670
Coordinates   3954208..3954642 (-) Length   144 a.a.
NCBI ID   WP_000811502.1    Uniprot ID   A0A5C5ACH0
Organism   Bacillus albus strain YK87     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3949208..3959642
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VSY18_RS20360 (VSY18_20360) - 3949613..3950203 (+) 591 WP_166688095.1 PadR family transcriptional regulator -
  VSY18_RS20365 (VSY18_20365) - 3950354..3950785 (-) 432 WP_166688094.1 NUDIX hydrolase -
  VSY18_RS20370 (VSY18_20370) metE 3951457..3953745 (+) 2289 WP_270673239.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  VSY18_RS20375 (VSY18_20375) comJ 3953783..3954172 (-) 390 WP_048530770.1 competence protein ComJ -
  VSY18_RS20380 (VSY18_20380) nucA/comI 3954208..3954642 (-) 435 WP_000811502.1 DNA-entry nuclease Machinery gene
  VSY18_RS20385 (VSY18_20385) - 3954879..3955628 (+) 750 WP_000388464.1 DUF3967 domain-containing protein -
  VSY18_RS20390 (VSY18_20390) kinB 3955682..3956956 (-) 1275 WP_048530774.1 sporulation sensor histidine kinase KinB -
  VSY18_RS20395 (VSY18_20395) - 3957265..3957465 (+) 201 WP_000929281.1 hypothetical protein -
  VSY18_RS20400 (VSY18_20400) - 3957589..3958752 (+) 1164 WP_166688093.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16010.38 Da        Isoelectric Point: 8.4331

>NTDB_id=926333 VSY18_RS20380 WP_000811502.1 3954208..3954642(-) (nucA/comI) [Bacillus albus strain YK87]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSIKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=926333 VSY18_RS20380 WP_000811502.1 3954208..3954642(-) (nucA/comI) [Bacillus albus strain YK87]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTGTATGAAGTACTTGTTCC
AGAGGAAACAAGTATCAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGCGATATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGACCGTGGTGGCGCTGCGGATAGACGAAAA
TTATCGTTAGCCCCATACCCATCAAAAAAAGGATATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCAGCAGATAACCGCGGAGCAGGCTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAGTTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C5ACH0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

71.171

77.083

0.549