Detailed information NTG0926315

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: VSY18_RS05940
• Genome accession: NZ_CP142670
• Coordinates: 1145327..1149052 (+)
• Length: 1241 a.a.
• NCBI ID: WP_270735046.1
• Uniprot ID: -
• Organism: Bacillus albus strain YK87
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1140327..1154052

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VSY18_RS05925 (VSY18_05925)	-	1140485..1141078 (+)	594	WP_048526648.1	TVP38/TMEM64 family protein	-
VSY18_RS05930 (VSY18_05930)	lepB	1141135..1141698 (+)	564	WP_048526650.1	signal peptidase I	-
VSY18_RS05935 (VSY18_05935)	addB	1141815..1145330 (+)	3516	WP_247998844.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
VSY18_RS05940 (VSY18_05940)	addA	1145327..1149052 (+)	3726	WP_270735046.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
VSY18_RS05945 (VSY18_05945)	-	1149065..1149352 (+)	288	WP_000255723.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
VSY18_RS05950 (VSY18_05950)	-	1149446..1149823 (-)	378	WP_048526657.1	DUF1433 domain-containing protein	-
VSY18_RS05955 (VSY18_05955)	-	1149882..1151051 (+)	1170	WP_071758310.1	hypothetical protein	-
VSY18_RS05960 (VSY18_05960)	-	1151062..1151442 (+)	381	WP_098344763.1	hypothetical protein	-
VSY18_RS05965 (VSY18_05965)	gerPF	1151721..1151936 (-)	216	WP_001141566.1	spore germination protein GerPF	-
VSY18_RS05970 (VSY18_05970)	-	1151979..1152365 (-)	387	WP_048526663.1	spore germination protein GerPE	-
VSY18_RS05975 (VSY18_05975)	gerPD	1152381..1152575 (-)	195	WP_001102342.1	spore germination protein GerPD	-
VSY18_RS05980 (VSY18_05980)	gerPC	1152582..1153196 (-)	615	WP_048526666.1	spore germination protein GerPC	-
VSY18_RS05985 (VSY18_05985)	gerPB	1153265..1153471 (-)	207	WP_001012508.1	spore germination protein GerPB	-
VSY18_RS05990 (VSY18_05990)	gerPA	1153486..1153707 (-)	222	WP_001111188.1	spore germination protein GerPA	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142632.69 Da
• Isoelectric Point: 4.8481

>NTDB_id=926315 VSY18_RS05940 WP_270735046.1 1145327..1149052(+) (addA) [Bacillus albus strain YK87]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVRFQLETAEQHI
RKATELAMLTDGPAPRVETLQADLALLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDIVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEHSENGEMNPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGSGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGATYPEGEDVAAELLCIQQTEEEVPDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEKELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPAEIYGYDTSWK
VEVVDGSTLLAPEPAQEEKQELLEALREKKAVPLQSERKDEVYDKLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIQTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITEEVIREQINGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVNIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=926315 VSY18_RS05940 WP_270735046.1 1145327..1149052(+) (addA) [Bacillus albus strain YK87]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAGTGGACAGATGACCAGTGGAAAGCCGTTGTAGCGAACGGACG
TGATATTTTAGTCGCGGCAGCAGCTGGTTCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGATGAACCAGGCTCTCAGCACATTAGAAAGCAGCTGAGCCTATTAAATAAAGC
TTCTATTTCGACGATCCATTCCTTTTGTTTACAAGTAATTAGAGGATACTATTACATGCTTGATGTTGATCCTCGTTTCC
GCATTGCGAATCAAACGGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGAATTGAA
GATAATACGATATTCTTTGAGCTCGTTGATCGTTATACGAGTGATCGTAGTGACGATGATTTACAGCGTATGATTTTAGC
ACTTCATACAGAATCAAGAGCACATCCAAATCCGGAAAAGTGGCTCGATAAATTAGTAGAAGCATATGACGTGGAAGGAA
AGACAATTGAAGATTTAGTGTATGCTTCTTATTTATTAGAAGATGTGAGATTCCAGCTTGAAACAGCGGAACAGCATATT
CGGAAAGCGACGGAACTCGCAATGCTTACTGACGGTCCAGCGCCTCGCGTTGAAACACTGCAAGCAGATTTAGCTTTACT
TGGAACGTTATCATCAGCCGCTCGTGAATCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAATGAAGATATTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGTTATTTAGCCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCACCCAGTATTAGA
AAAGCTTGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACATAGTGAAAACGGTGAAATGAATCCGTCAGCAGTAGCGCTTCAA
TATCGTAATAAATTTGCTGAAGTACTAGTCGATGAATATCAAGATACGAACTTCGTACAGGAATCTATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTAGGTGACGTAAAACAGTCAATTTATCGTTTCCGACTAGCAG
AACCAGGTTTATTCCTAGGAAAGTATAAACGCTTTACACAAGAAGGATCGGGCGGCGGAATGAAGATAGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCAGGTACGAACTTTATCTTCAAACAAATTATGGGTGAAGAAGTCGGAGAAAT
CGACTACGATGCAGACGCTGAATTAAAGTTAGGTGCTACCTATCCAGAAGGTGAAGATGTAGCGGCTGAACTACTATGTA
TTCAGCAAACGGAAGAAGAAGTGCCAGACGGTGAAGAAGGTGCCGAAGTAGAAAAAGCACAGCTTGAAGCTCGACTTATG
GCGCAGCGTATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGACCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGTGACTTCGTTATTTTGCTTCGCTCTATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAGTTGCAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATAATGATGAACGTATTCCGCGTGATCGATAAT
CCGATGCAAGATATTCCGCTTGCAGCAGTGCTTCGTTCACCAATCGTTGGATTAAATGATGAAGAACTTGCAACGCTTCG
TGCCCACGGAAAGAAAGGTTCGTTTTATGAAGTGATGAGCTCATTCTTAAAAGGAGCACCGCTTGAAGAAGAAAAAGAAC
TACATGATAAATTAGAGTGGTTCTATAACTTACTACAAGGATGGCGTGAATTCGCACGTCAACAGTCTCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTTGTCGGTGGTTTACCAGCCGGAAAGCAAAGACAGGCAAACTT
GCGTGTATTATATGACCGTGCAAGACAATACGAAGCAACATCGTTTAGAGGACTATTCCGCTTCTTACGTTTTATTGAAC
GTATTTTAGAACGCGGTGACGATATGGGTACGGCGAGAGCTTTAGGTGAACAAGAAGACGTCGTTCGCATTATGACGATT
CATAAAAGTAAAGGGCTTGAGTTCCCAGTCGTATTCGTAGCGGGGCTTGGTCGCCGTTTTAACACACAAGATTTAATGAA
ACGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCACAATTTATCGATCCTCGTAAACGTATTAAATATACGACATTAT
CGCAACTAGCAATTAAGCGTAAAATGAAAATGGAATTAATCGCGGAAGAAATGCGCGTATTATACGTAGCGTTAACGCGT
GCAAAAGAAAAGTTAATTTTAATTGGAACAGTGAAGGATGCAAATAAGGAAATGGAAAAATGGCTTGATGCGAGAGAGCA
TAGTGAATGGTTATTACCAGACCATATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGTAGTATTCCGGCTGAAATTTATGGATACGACACTAGCTGGAAA
GTAGAAGTTGTTGACGGTAGCACGTTACTTGCGCCAGAACCAGCTCAAGAAGAGAAACAAGAATTACTAGAAGCACTTCG
TGAGAAAAAAGCTGTTCCCCTGCAAAGTGAACGAAAAGATGAAGTGTACGACAAATTAATGTGGAAGTACGGATATGAGG
AAGCAACATCTCATCGTGCGAAACAGTCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAACTGCGTGCGCCAATTCAAACACGCCCTCGTTTTATGGAGAAAAAAGGATTAACGTACGCTGAAAGAGG
TACGGCAGTTCACGCTGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACAGAAGAAGTGATTCGGGAACAAATTAATG
GCATGGTCAATAAAGAGCTATTAACATTCGAACAAGCAGAAGAAATAGCAATCGAAAAAGTCATTTCATTCTTTGACAGT
GATCTAGGTAAAAGAGTATTAGCGGCGAAAAGCGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGAAAGAGCGGGGAATCAATTCTTGTTCAAGGGGTTATTGACTGTATGATTGAAGAAGAAGACG
GTATTACGTTAATCGATTTTAAAACGGATACGATTGAAGGGAAGTTCCCAGGCGGATTTGATCAAGCGAAACCAATTTTA
GAAGATCGATATAAAGTGCAACTTTCTTTATATGCAAAGGCACTTGAAAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATATTTCTTTGATGGGAATCACGTTGTAAATATTGAAGAATAG

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.666	100	0.537