Detailed information NTG0926083

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: VVB72_RS09230
• Genome accession: NZ_CP142589
• Coordinates: 1867988..1868635 (-)
• Length: 215 a.a.
• NCBI ID: WP_010906131.1
• Uniprot ID: A0A3N6KQH1
• Organism: Lactococcus lactis subsp. lactis strain C20
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1862988..1873635

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VVB72_RS09205 (VVB72_09205)	atpF	1863057..1863563 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
VVB72_RS09210 (VVB72_09210)	atpB	1863578..1864291 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
VVB72_RS09215 (VVB72_09215)	-	1864336..1864551 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
VVB72_RS09220 (VVB72_09220)	-	1864736..1865512 (-)	777	WP_039115826.1	alpha/beta hydrolase	-
VVB72_RS09225 (VVB72_09225)	comEC	1865797..1868007 (-)	2211	WP_039115828.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
VVB72_RS09230 (VVB72_09230)	comEA	1867988..1868635 (-)	648	WP_010906131.1	ComEA family DNA-binding protein	Machinery gene
VVB72_RS09235 (VVB72_09235)	-	1868695..1870056 (-)	1362	WP_039115830.1	ABC transporter permease	-
VVB72_RS09240 (VVB72_09240)	-	1870053..1870985 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
VVB72_RS09245 (VVB72_09245)	-	1871090..1871488 (-)	399	WP_023188528.1	hypothetical protein	-
VVB72_RS09250 (VVB72_09250)	-	1871592..1872155 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
VVB72_RS09255 (VVB72_09255)	-	1872328..1873506 (-)	1179	WP_012898345.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23624.76 Da
• Isoelectric Point: 4.8659

>NTDB_id=926083 VVB72_RS09230 WP_010906131.1 1867988..1868635(-) (comEA) [Lactococcus lactis subsp. lactis strain C20]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=926083 VVB72_RS09230 WP_010906131.1 1867988..1868635(-) (comEA) [Lactococcus lactis subsp. lactis strain C20]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATCTAATTAAGGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGATAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A3N6KQH1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	76.147	100	0.772
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386