Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   U6115_RS09565 Genome accession   NZ_CP142381
Coordinates   1929699..1931060 (+) Length   453 a.a.
NCBI ID   WP_043576018.1    Uniprot ID   -
Organism   Chromobacterium subtsugae strain ATCC 31532     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1924699..1936060
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U6115_RS09540 (U6115_09540) hpnC 1925312..1926124 (+) 813 WP_043576030.1 squalene synthase HpnC -
  U6115_RS09545 (U6115_09545) hpnD 1926192..1927037 (+) 846 WP_043576029.1 presqualene diphosphate synthase HpnD -
  U6115_RS09550 (U6115_09550) hpnE 1927034..1928311 (+) 1278 WP_043576026.1 hydroxysqualene dehydroxylase HpnE -
  U6115_RS09555 (U6115_09555) - 1928308..1929063 (+) 756 WP_043576024.1 SDR family oxidoreductase -
  U6115_RS09560 (U6115_09560) - 1929080..1929619 (+) 540 WP_043576021.1 hypothetical protein -
  U6115_RS09565 (U6115_09565) radA 1929699..1931060 (+) 1362 WP_043576018.1 DNA repair protein RadA Machinery gene
  U6115_RS09570 (U6115_09570) - 1931067..1931627 (+) 561 WP_043576016.1 sigma-70 family RNA polymerase sigma factor -
  U6115_RS09575 (U6115_09575) - 1931624..1931803 (+) 180 WP_043576004.1 zf-HC2 domain-containing protein -
  U6115_RS09580 (U6115_09580) - 1931902..1932798 (+) 897 WP_059184263.1 hypothetical protein -
  U6115_RS09585 (U6115_09585) rlmF 1932893..1933879 (+) 987 WP_043576000.1 23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF -
  U6115_RS09590 (U6115_09590) - 1933957..1934307 (-) 351 WP_043575998.1 DUF2325 domain-containing protein -
  U6115_RS09595 (U6115_09595) - 1934556..1935341 (+) 786 WP_043575996.1 transporter substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 48557.87 Da        Isoelectric Point: 6.9341

>NTDB_id=925624 U6115_RS09565 WP_043576018.1 1929699..1931060(+) (radA) [Chromobacterium subtsugae strain ATCC 31532]
MAKTKTVFSCTECGGQTPKWQGQCPHCNAWNTLTEAVSTPAAAGPRFQSWAANVTKVQRLSEVQTEETPRDPSGIDELDR
VLGGGIVRGAVVLIGGDPGIGKSTLLLQALSVIGQRRKVLYVSGEESAQQIALRASRLALDTSSVNLLAEICIENILATL
KNDPPEVVVIDSIQTLYTEHVTSAPGSVSQVRECAAQLTRMAKQSGITVLLVGHVTKDGSLAGPRVLEHMVDTVLYFEGD
SHSSHRMIRAIKNRFGAVNELGVFAMTDRGLKGVSNPSAIFLSSYRDDVAGSCVLVTQEGTRPLLVEVQALVDDCHGGQP
KRLAVGLEQNRLSMLLAVLHRHGGVACFDQDVFLNAVGGVKIIEPAADLAIILAMVSSLRDKPLPEKLVVFGEVGLAGEV
RPVARGQERLKEAAKLGFTRAIVPTANKPRQEIEGLKVLAVDRLDQAVEFCRE

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=925624 U6115_RS09565 WP_043576018.1 1929699..1931060(+) (radA) [Chromobacterium subtsugae strain ATCC 31532]
ATGGCCAAAACCAAAACCGTATTCAGCTGCACCGAGTGTGGCGGGCAGACGCCCAAATGGCAGGGCCAATGCCCGCATTG
CAATGCCTGGAACACCCTGACCGAAGCGGTGTCCACGCCGGCGGCGGCCGGCCCGCGCTTCCAGTCGTGGGCGGCCAATG
TCACCAAGGTGCAGCGGCTGTCCGAAGTGCAGACCGAGGAAACCCCGCGCGACCCATCCGGCATCGACGAGCTGGACCGG
GTGCTGGGCGGCGGCATCGTGCGCGGCGCGGTGGTGCTGATCGGCGGCGACCCCGGTATCGGCAAGTCCACGCTGCTGTT
GCAGGCGCTGTCGGTGATAGGCCAGCGCCGCAAGGTGCTGTACGTGTCGGGCGAGGAGTCGGCGCAGCAGATCGCGCTGC
GCGCCTCGCGGCTGGCGCTGGATACCAGCAGCGTCAACCTGCTGGCGGAAATCTGCATAGAAAACATCCTGGCCACCCTG
AAGAACGACCCGCCGGAAGTGGTGGTGATCGACTCGATCCAGACGCTGTACACCGAGCACGTCACCTCGGCGCCGGGCTC
GGTGTCGCAGGTGCGCGAATGCGCGGCGCAACTGACGCGGATGGCCAAGCAAAGCGGCATCACCGTGCTGCTGGTGGGCC
ACGTCACCAAGGACGGCTCGCTGGCCGGCCCGCGCGTGCTGGAGCACATGGTGGACACCGTGCTGTATTTCGAGGGCGAT
TCGCATTCCAGCCACCGGATGATACGCGCGATCAAGAACCGCTTCGGCGCGGTCAACGAGCTGGGCGTGTTCGCGATGAC
CGACCGCGGCCTGAAAGGCGTGTCCAATCCGTCGGCCATCTTCCTGTCCTCGTACCGCGACGATGTCGCCGGCTCCTGCG
TGCTGGTGACCCAGGAAGGCACCCGCCCGCTGCTAGTGGAGGTGCAGGCGCTGGTGGACGATTGCCACGGCGGCCAGCCC
AAGCGGCTGGCGGTGGGCCTGGAGCAGAACCGGCTGTCCATGTTGCTGGCGGTGCTGCACCGCCACGGCGGCGTCGCCTG
TTTCGACCAGGATGTGTTCCTCAACGCGGTGGGCGGGGTGAAGATCATCGAGCCGGCGGCCGACCTCGCCATCATCCTGG
CAATGGTGTCGTCGCTGCGCGACAAGCCCTTGCCGGAAAAGCTGGTGGTGTTCGGTGAAGTAGGGCTGGCCGGCGAAGTG
CGGCCGGTGGCGCGCGGGCAGGAGCGGCTGAAGGAAGCGGCCAAGCTCGGCTTCACCCGCGCCATCGTGCCGACGGCCAA
CAAGCCGCGCCAGGAAATCGAGGGCCTGAAAGTGCTGGCGGTGGACCGCCTGGACCAGGCGGTGGAATTCTGCAGGGAGT
AG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(64-219)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(365-446)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.439

100

0.508
  radA Streptococcus mitis SK321

47.788

99.779

0.477
  radA Streptococcus mitis NCTC 12261

47.566

99.779

0.475
  radA Streptococcus pneumoniae Rx1

49.528

93.598

0.464
  radA Streptococcus pneumoniae D39

49.528

93.598

0.464
  radA Streptococcus pneumoniae R6

49.528

93.598

0.464
  radA Streptococcus pneumoniae TIGR4

49.528

93.598

0.464