Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PanNE5_RS04200 Genome accession   NZ_AP025517
Coordinates   911092..912579 (+) Length   495 a.a.
NCBI ID   WP_017232647.1    Uniprot ID   A0A9E9DAH4
Organism   Pandoraea sp. NE5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 906092..917579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PanNE5_RS04175 (PanNE5_08370) - 907250..908230 (+) 981 WP_017232643.1 KpsF/GutQ family sugar-phosphate isomerase -
  PanNE5_RS04180 (PanNE5_08380) - 908338..908823 (+) 486 WP_051060087.1 KdsC family phosphatase -
  PanNE5_RS04185 (PanNE5_08390) lptC 908847..909506 (+) 660 WP_017232645.1 LPS export ABC transporter periplasmic protein LptC -
  PanNE5_RS04190 (PanNE5_08400) lptA 909503..910201 (+) 699 WP_150562278.1 lipopolysaccharide transport periplasmic protein LptA -
  PanNE5_RS04195 (PanNE5_08410) lptB 910227..910997 (+) 771 WP_026131715.1 LPS export ABC transporter ATP-binding protein -
  PanNE5_RS04200 (PanNE5_08420) rpoN/rpoN1 911092..912579 (+) 1488 WP_017232647.1 RNA polymerase factor sigma-54 Machinery gene
  PanNE5_RS04205 (PanNE5_08430) hpf 912722..913075 (+) 354 WP_017232648.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  PanNE5_RS04210 (PanNE5_08440) ptsN 913305..913760 (+) 456 WP_026131716.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PanNE5_RS04215 (PanNE5_08450) hprK 913858..914823 (+) 966 WP_017232650.1 HPr(Ser) kinase/phosphatase -
  PanNE5_RS04220 (PanNE5_08460) - 914970..915281 (+) 312 WP_261939298.1 PsiF family protein -
  PanNE5_RS04225 (PanNE5_08470) rapZ 915512..916387 (+) 876 WP_017232652.1 RNase adapter RapZ -
  PanNE5_RS04230 (PanNE5_08480) mutY 916403..917560 (-) 1158 WP_017232653.1 A/G-specific adenine glycosylase -

Sequence

Protein


Download         Length: 495 a.a.        Molecular weight: 55303.24 Da        Isoelectric Point: 5.1872

>NTDB_id=92446 PanNE5_RS04200 WP_017232647.1 911092..912579(+) (rpoN/rpoN1) [Pandoraea sp. NE5]
MKPTLQLRTSQHLTLTPQLQQSIRLLQLSTLELQQEVEHALTQNPMLEREDDWLAGTIRVGTDGSLRNERGSSSSSTNDT
PESASGQDDARELDGETRFDDNARDGGNDWSLNDFARSGSASDDDDNERPQLHVDHPNLREHLLAQLRLTKASPRDRLLV
EFLIESLDEDGYLGTLLDEMQAEMSEELGLETEEINAALALLQSFDPAGVGARTPSECLRLQLQRLPASSVRTLSLRIVS
DHLELLAARDYTRLRRLLGVSEDSLRAAHQLIRSLDPFPGAAYGSPQADYVIPDVLVRKQGGGWTAELNPEVMPRLRINE
MYARILRNNRNAPGTGNLQQQLQEARWLIKNIQQRFDTILRVSQAIVERQKNFFTHGEIGMRPLVLREIADTLGLHESTI
SRVTTSKYMLTPFGTFELKYFFGSHVATEAGGAASSTAIRALIKQLIGAEDPQTPLSDSRIAELLAEQGFVVARRTVAKY
REAMRIPAVNLRKSL

Nucleotide


Download         Length: 1488 bp        

>NTDB_id=92446 PanNE5_RS04200 WP_017232647.1 911092..912579(+) (rpoN/rpoN1) [Pandoraea sp. NE5]
ATGAAACCGACCCTTCAACTCCGCACCTCCCAGCACCTGACCCTCACGCCTCAGTTGCAGCAATCCATCCGATTGTTGCA
ACTGTCGACGCTCGAACTTCAGCAGGAAGTCGAGCACGCCCTCACCCAGAACCCGATGCTCGAGCGCGAGGACGATTGGC
TCGCCGGCACGATCCGTGTGGGCACGGACGGATCGCTGCGCAATGAGCGGGGCTCGTCGAGCAGCAGCACCAACGACACG
CCGGAATCGGCCAGCGGCCAGGACGACGCCCGCGAACTCGACGGCGAGACCCGCTTCGACGACAACGCGCGCGACGGTGG
CAACGACTGGAGCCTGAACGACTTTGCCCGCTCGGGCAGTGCGTCGGACGACGACGACAATGAGCGGCCGCAACTGCACG
TCGATCATCCCAATCTTCGCGAACACCTGCTCGCCCAGTTGCGCCTGACGAAAGCCAGCCCGCGCGATCGCCTGCTGGTG
GAATTCCTGATCGAATCGCTCGACGAAGACGGCTATCTCGGCACCCTGCTCGACGAAATGCAGGCGGAAATGTCCGAGGA
ACTTGGGCTCGAGACCGAAGAGATCAACGCCGCGCTCGCGCTGCTGCAGAGCTTCGACCCGGCAGGCGTGGGCGCCCGAA
CCCCGTCAGAATGCCTTCGCCTGCAACTCCAGCGGCTGCCGGCAAGCAGCGTGCGAACTCTGTCGCTGCGCATCGTTTCC
GATCATCTGGAGCTGCTCGCCGCGCGCGACTACACACGGTTGCGTCGTCTGCTGGGCGTGAGCGAGGACAGCCTGCGCGC
GGCGCATCAACTGATTCGCTCGCTCGACCCGTTCCCGGGCGCCGCCTACGGCTCGCCCCAGGCGGATTACGTCATCCCCG
ACGTGCTCGTGCGCAAGCAAGGCGGCGGCTGGACCGCCGAACTCAATCCCGAGGTCATGCCGCGCCTGCGCATCAATGAA
ATGTATGCGCGCATCCTTCGCAACAACCGCAATGCGCCGGGAACCGGCAACCTGCAGCAGCAACTTCAGGAAGCGCGCTG
GCTCATCAAGAACATCCAACAGCGGTTCGACACGATCCTGCGCGTGTCGCAGGCGATCGTCGAGCGTCAGAAGAACTTCT
TCACGCATGGCGAAATCGGCATGCGGCCCTTGGTTTTACGCGAAATTGCTGATACGCTGGGTTTACATGAATCCACGATT
TCACGCGTGACAACCAGTAAGTACATGCTCACTCCATTCGGAACCTTCGAGCTTAAGTACTTCTTTGGTTCACACGTGGC
CACGGAAGCCGGGGGCGCCGCCTCATCGACCGCCATCCGCGCACTCATCAAGCAACTCATAGGAGCAGAAGATCCGCAGA
CCCCCCTTTCCGACAGCCGTATCGCGGAGCTGCTGGCAGAGCAAGGGTTCGTGGTAGCGCGACGCACCGTCGCGAAATAC
CGCGAAGCCATGCGCATTCCCGCAGTGAACTTGCGCAAATCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(338-493)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(133-322)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.465

100

0.683

Multiple sequence alignment