Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DPPLL_RS17275 Genome accession   NZ_AP025516
Coordinates   3827507..3828853 (+) Length   448 a.a.
NCBI ID   WP_284154659.1    Uniprot ID   -
Organism   Desulfofustis limnaeus strain PPLL     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3822507..3833853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DPPLL_RS17255 (DPPLL_34650) gatB 3823128..3824552 (+) 1425 WP_284152425.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  DPPLL_RS17260 (DPPLL_34660) radC 3824562..3825290 (+) 729 WP_284152426.1 DNA repair protein RadC -
  DPPLL_RS17265 (DPPLL_34670) hemW 3825303..3826430 (+) 1128 WP_284152427.1 radical SAM family heme chaperone HemW -
  DPPLL_RS17270 (DPPLL_34680) - 3826451..3827242 (-) 792 WP_284152428.1 EI24 domain-containing protein -
  DPPLL_RS17275 (DPPLL_34690) radA 3827507..3828853 (+) 1347 WP_284154659.1 DNA repair protein RadA Machinery gene
  DPPLL_RS17280 (DPPLL_34700) - 3828924..3829565 (+) 642 WP_284152429.1 hypothetical protein -
  DPPLL_RS17285 (DPPLL_34710) lon 3829855..3832296 (+) 2442 WP_284152430.1 endopeptidase La -
  DPPLL_RS17290 (DPPLL_34720) - 3832538..3833806 (+) 1269 WP_284152431.1 cyclic nucleotide-binding domain-containing protein -

Sequence

Protein


Download         Length: 448 a.a.        Molecular weight: 47855.24 Da        Isoelectric Point: 6.3190

>NTDB_id=92442 DPPLL_RS17275 WP_284154659.1 3827507..3828853(+) (radA) [Desulfofustis limnaeus strain PPLL]
MAFVCRECGHQSGRWLGRCPGCGQWECMVEEEVVGAGRSRSHEYVEPQPLTMAPDGDHQRIVIGVSELDRVLGGGIVPGS
VILIGGDPGIGKSTLLLQLLSALAARGHQVLYVSGEESAHQIKSRAKRLSAVHERHLLATETAVERIAAMTTAIRPMVVA
IDSIQTLTCADYPSSPGSVTQVRESASYLLDLAKKENIPMILVGHVTKEGTLAGPRVLEHMVDTVLYFEGDRSHAFRILR
TVKNRFGSTNEIGVFSMKEEGLVPVANPSELFLAERPLDVAGSVVLATMEGTRPILVEVQALVSPTNLGTARRTAAGADP
KRLALLCAVLEKKAGLDFYGHDIFLNIAGGMQIDEPALDLGVIGALASSLLERPVAADTVLAGEIGLAGEIRAVGHMEQR
VAEAERLGFKHFIMPKTNADRLAAGSTNIRVMGVATVDEFLKVIASSL

Nucleotide


Download         Length: 1347 bp        

>NTDB_id=92442 DPPLL_RS17275 WP_284154659.1 3827507..3828853(+) (radA) [Desulfofustis limnaeus strain PPLL]
ATGGCCTTTGTCTGTCGGGAATGCGGCCATCAAAGTGGACGTTGGTTGGGCCGCTGCCCTGGCTGCGGCCAGTGGGAGTG
CATGGTCGAGGAGGAGGTCGTTGGTGCCGGTCGTAGCCGAAGCCATGAATATGTGGAGCCGCAGCCGCTGACCATGGCAC
CGGACGGGGATCACCAACGCATCGTCATCGGCGTGAGCGAGCTTGACCGGGTACTCGGCGGCGGGATCGTGCCTGGGTCG
GTTATTTTGATCGGAGGCGATCCCGGCATCGGCAAATCAACCCTGCTGCTGCAGTTGTTGTCGGCGCTGGCCGCTCGGGG
GCACCAGGTACTCTACGTCTCCGGTGAGGAATCGGCACATCAGATCAAGTCGCGAGCGAAACGGTTGTCTGCCGTTCATG
AGAGGCACCTGTTGGCGACCGAAACGGCCGTGGAACGCATCGCGGCCATGACCACCGCCATCAGGCCGATGGTGGTCGCG
ATTGATTCGATCCAAACGCTGACCTGTGCCGATTACCCATCATCGCCGGGCTCGGTGACCCAGGTCCGTGAATCCGCCTC
GTATCTCCTTGATTTGGCCAAGAAGGAAAATATCCCGATGATCCTGGTCGGGCATGTGACCAAGGAGGGAACCCTGGCTG
GCCCGCGAGTCCTCGAGCATATGGTCGATACGGTGCTGTATTTCGAGGGCGATCGAAGTCACGCATTTCGTATTCTCCGC
ACGGTAAAAAACCGTTTCGGCTCGACCAACGAGATCGGGGTTTTCTCGATGAAGGAGGAAGGCCTGGTCCCAGTGGCCAA
TCCATCGGAGCTGTTTCTCGCCGAACGGCCGCTGGATGTTGCCGGTTCGGTAGTGCTGGCGACGATGGAAGGGACACGAC
CGATTCTCGTGGAAGTGCAAGCTCTGGTAAGCCCAACCAACCTGGGGACGGCGAGGAGGACGGCGGCGGGAGCCGATCCG
AAACGGTTGGCGCTGCTCTGTGCCGTCTTGGAAAAAAAGGCTGGCCTGGATTTTTACGGCCACGATATCTTTTTGAATAT
TGCCGGTGGCATGCAGATCGACGAACCGGCGCTCGACCTCGGGGTTATCGGCGCGCTCGCCTCGAGCCTCCTGGAGCGGC
CGGTGGCTGCCGACACGGTCCTCGCCGGAGAAATCGGCCTGGCTGGAGAGATTCGTGCGGTCGGGCACATGGAACAGCGG
GTTGCCGAAGCTGAGCGGCTTGGCTTCAAACACTTTATCATGCCCAAGACCAATGCCGACCGGTTGGCAGCGGGTTCGAC
GAATATACGCGTCATGGGGGTTGCAACGGTGGACGAATTCCTTAAGGTAATAGCGAGTTCCCTTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(3-30)

AAA domain (AAA_25)

(70-210)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(342-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae R6

51.902

99.777

0.518
  radA Streptococcus pneumoniae Rx1

51.902

99.777

0.518
  radA Streptococcus pneumoniae D39

51.902

99.777

0.518
  radA Streptococcus pneumoniae TIGR4

51.902

99.777

0.518
  radA Streptococcus mitis SK321

51.902

99.777

0.518
  radA Streptococcus mitis NCTC 12261

51.678

99.777

0.516
  radA Bacillus subtilis subsp. subtilis str. 168

51.23

99.777

0.511

Multiple sequence alignment