Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AB1N70_RS02660 Genome accession   NZ_CP162603
Coordinates   563820..565253 (-) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain PEC1020     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 558820..570253
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB1N70_RS02625 elbB 559512..560165 (+) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -
  AB1N70_RS02630 mtgA 560162..560890 (+) 729 WP_020233285.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  AB1N70_RS02635 yrbL 560887..561519 (-) 633 WP_000620396.1 PhoP regulatory network protein YrbL -
  AB1N70_RS02640 npr 561732..562004 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  AB1N70_RS02645 rapZ 562001..562855 (-) 855 WP_000243741.1 RNase adapter RapZ -
  AB1N70_RS02650 ptsN 562901..563392 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AB1N70_RS02655 hpf 563510..563797 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  AB1N70_RS02660 rpoN/rpoN1 563820..565253 (-) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  AB1N70_RS02665 lptB 565301..566026 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  AB1N70_RS02670 lptA 566033..566590 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  AB1N70_RS02675 lptC 566559..567134 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  AB1N70_RS02680 kdsC 567131..567697 (-) 567 WP_000030006.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  AB1N70_RS02685 kdsD 567718..568704 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  AB1N70_RS02690 yrbG 568718..569695 (-) 978 WP_000922873.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=923947 AB1N70_RS02660 WP_000809051.1 563820..565253(-) (rpoN/rpoN1) [Escherichia coli strain PEC1020]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=923947 AB1N70_RS02660 WP_000809051.1 563820..565253(-) (rpoN/rpoN1) [Escherichia coli strain PEC1020]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CTGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCACTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCAGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAAACCACGCCATGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACGGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTTAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465