Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   AB3433_RS02365 Genome accession   NZ_CP162469
Coordinates   434425..436698 (+) Length   757 a.a.
NCBI ID   WP_000594997.1    Uniprot ID   -
Organism   Staphylococcus aureus strain TUM20820     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 429425..441698
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3433_RS02350 (AB3433_02350) tgt 430250..431389 (+) 1140 WP_001112045.1 tRNA guanosine(34) transglycosylase Tgt -
  AB3433_RS02355 (AB3433_02355) yajC 431408..431668 (+) 261 WP_001160682.1 preprotein translocase subunit YajC -
  AB3433_RS02360 (AB3433_02360) secDF 431943..434222 (+) 2280 WP_000749795.1 protein translocase subunit SecDF -
  AB3433_RS02365 (AB3433_02365) recJ 434425..436698 (+) 2274 WP_000594997.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  AB3433_RS02370 (AB3433_02370) - 436720..437238 (+) 519 WP_000364542.1 adenine phosphoribosyltransferase -
  AB3433_RS02375 (AB3433_02375) - 437666..439855 (+) 2190 WP_001058587.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  AB3433_RS02380 (AB3433_02380) dtd 439867..440319 (+) 453 WP_000869983.1 D-aminoacyl-tRNA deacylase -
  AB3433_RS02385 (AB3433_02385) - 440316..441191 (+) 876 WP_000717800.1 N-acetylmuramoyl-L-alanine amidase -

Sequence

Protein


Download         Length: 757 a.a.        Molecular weight: 85630.85 Da        Isoelectric Point: 4.8732

>NTDB_id=923358 AB3433_RS02365 WP_000594997.1 434425..436698(+) (recJ) [Staphylococcus aureus strain TUM20820]
MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQAIESIISDTDINHDALQLSDMTKTIERIKRAIANDE
KILVYGDYDADGVTSTTILVTTLQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFKAIVAIGTIADLVSLTDENRSLV
KQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFIIGPRLNAVGRLDDASLACELLMTDDEEEAAFLAEQVEHFNRERK
DIVATITEEAMAMAETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSIDQVSMFEIL
SAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVKQVDVLLTENDITIKNIRDMNRLRPFGTDFS
RPIFEMDDLSVSSVKAIGQQKNHLKLTLGESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQIIIQDIAMN
EQQILDYRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDLSNSLQQLQFSQLYIVL
QHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCQHLSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRS
IDSSKVYQLRQQRMDVEKQLLYQDFSEIKNWIKSQLS

Nucleotide


Download         Length: 2274 bp        

>NTDB_id=923358 AB3433_RS02365 WP_000594997.1 434425..436698(+) (recJ) [Staphylococcus aureus strain TUM20820]
ATGATTAAACCGAAGTATAAGTGGAAATTAACTAAACCAGCTGAATACATAAGTGATGAATTAACATCAAAATTAAAGTT
AACGCCAATCGTAAAAAAAATATTAGAAAGTAAATCGATTATTGATGAACAAGCAATAGAATCCATCATTTCAGATACAG
ATATCAATCATGATGCATTGCAATTGAGCGATATGACTAAGACTATTGAAAGAATTAAAAGAGCGATAGCGAATGATGAG
AAAATCCTAGTTTATGGTGATTACGATGCTGACGGTGTTACCTCAACTACAATTTTAGTGACAACATTACAACTTTTAGG
TGCACAAGTAGGATGGCATATTCCTAACCGCTTTACCGAAGGATATGGACCAAACGAACTAGCATTTAGAAATGCTCATG
ATGAAGGCATAACATTAATCATAACTGTTGATAATGGCATACAAGGTCATAATGAAATTAAAATGGTTCAAGATTTAGGA
GTTGATGTCATTGTAACTGATCATCATGAAATTGGTAGTACATTACCAGAAGCATATGCAATCGTACATCCAATGCATCC
ATCGTTTAATTATCCGTTCCAACAATTGTGTGGTGCAGGTGTTGCATACAAATTAGCACAAGCATTGATTGAAAATGTAC
CTGATTATTTTAAAGCAATAGTTGCGATAGGTACCATTGCGGATTTAGTATCCTTAACTGATGAAAATCGATCATTAGTG
AAACAAGGATTAAAAGTCTTAAATGATCAATGTCCGACCTCTGTTAAAGCATTGCTAAAAGAAGCGGGATACAATGACAA
TATTGATGAAGAAACAATTGGTTTTATTATTGGACCTAGACTTAATGCTGTAGGTCGTTTAGATGACGCATCATTAGCTT
GCGAATTGTTGATGACTGACGACGAAGAAGAAGCTGCATTTTTAGCAGAACAAGTTGAACATTTTAACCGTGAAAGAAAA
GATATTGTAGCAACTATCACAGAAGAAGCAATGGCTATGGCAGAAACGAAAGTTAAAAAGGGAGATTTATTTTTACTTTT
AGCAAAAGAAAATTGGCATGAAGGTGTCTTAGGTATTGTTGCTTCGAAAATTGTCGAAACATTTGCTTTGCCAACATTAA
TTTTAAATATTGATAGGGAGCAAAATCATGCAAAAGGTTCCGCCAGATCAATTGATCAAGTGTCGATGTTTGAAATACTA
TCAGCACATCAAGAGTTAATCGCTAAATTTGGCGGCCATCATATGGCTGCAGGTATGACGATGGATATTGAAAATATAGA
ATCGTTAGCTGAAGGTTTAAATAAATGGATGAAAGAATTATCTAAAACAACTTCGTTAGATCCCGTAAAGCAAGTAGATG
TATTATTAACAGAAAATGATATAACGATTAAAAATATTCGTGATATGAATCGTTTAAGACCCTTCGGAACAGATTTTTCT
AGACCTATTTTTGAAATGGATGATTTATCAGTTTCTTCAGTTAAGGCGATAGGTCAACAAAAAAATCATCTAAAATTGAC
ACTTGGTGAAAGTAATATCGCCGCGCTATTTTGGCAAAATGGACACTTAGAACCTGAGTTACAAGATGAACAGCCAATTA
ATATATTAGGATCTGTTCAAATCAACGAATGGAATGGTAATCAATCACCGCAAATAATTATTCAAGATATTGCGATGAAT
GAACAGCAAATATTAGATTATAGAAGTAAGCGAAAAAGTTTACCTTTTACAGAAAATGATGAAAATATTGTCGTGCTTAT
TCATCCTAAAAGTGATAAAGTAAATGCGAATGAATATTATTATGGTGAAGAAATTAAACAACAAACTGATAAAGTAGTAT
TAAGAGATTTACCAACGTCAATGGAAGACTTGTCTAATTCCTTGCAACAACTGCAATTTTCTCAACTTTATATAGTTTTG
CAACATAATCATTCGATTTACTTCGATGGTATACCTAATATGGATATTTTTAAAAAGTGTTATAAAGCATTAATAACTAA
ACAAGAAACAAATATCCAGAAAGAGGGTATGTTATTGTGTCAACATTTAAGTGTGAAACCAGATACACTTAAATTCATGT
TGAAAGTTTTCTTAGACTTAAAATTTGTAACACAAGAAGATGGTTTAATTCGAATCAATCAACAACCTGATAAAAGATCG
ATTGATTCCAGCAAAGTATATCAATTAAGACAACAACGTATGGATGTTGAAAAGCAATTATTATATCAAGATTTTTCAGA
AATAAAAAATTGGATAAAGTCACAATTGTCGTGA

Domains

Predicted by InterProScan.

DHH family (DHH)

(81-222)

DHHA1 domain (DHHA1)

(312-436)

RecJ OB domain (RecJ_OB)

(452-556)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(563-752)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

37.758

100

0.387
  recJ Streptococcus pneumoniae strain D39V

37.895

100

0.38