Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   VSF34_RS01410 Genome accession   NZ_CP142146
Coordinates   260492..261859 (+) Length   455 a.a.
NCBI ID   WP_326717342.1    Uniprot ID   -
Organism   Vagococcus jeotgali strain B2T-5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 255492..266859
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VSF34_RS01385 - 257054..258301 (-) 1248 WP_326717339.1 glutamate-5-semialdehyde dehydrogenase -
  VSF34_RS01390 proB 258298..259065 (-) 768 WP_326717992.1 glutamate 5-kinase -
  VSF34_RS01395 - 259396..259581 (+) 186 Protein_250 KUP/HAK/KT family potassium transporter -
  VSF34_RS01400 - 259625..259777 (+) 153 WP_326717340.1 hypothetical protein -
  VSF34_RS01405 - 259877..260404 (+) 528 WP_326717341.1 dUTP diphosphatase -
  VSF34_RS01410 radA 260492..261859 (+) 1368 WP_326717342.1 DNA repair protein RadA Machinery gene
  VSF34_RS01415 - 261877..262965 (+) 1089 WP_326717343.1 PIN/TRAM domain-containing protein -
  VSF34_RS01420 gltX 263075..264550 (+) 1476 WP_326717344.1 glutamate--tRNA ligase -
  VSF34_RS01425 cysS 264797..266215 (+) 1419 WP_326717345.1 cysteine--tRNA ligase -
  VSF34_RS01430 - 266212..266619 (+) 408 WP_326717346.1 Mini-ribonuclease 3 -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 49913.14 Da        Isoelectric Point: 6.3207

>NTDB_id=921971 VSF34_RS01410 WP_326717342.1 260492..261859(+) (radA) [Vagococcus jeotgali strain B2T-5]
MAKKKNEYVCQQCGYKSPKYLGRCPNCGEWNTLVEEIIQDKPTRHSRVNLSGEGSKVQKLKEVTSQKEPRVKTNLEELNR
VLGGGVVPGSMVLIGGDPGIGKSTLLLQVSQQLSLLDGNVLYVSGEESSEQIKMRAERLQDGSNDFYVYPETDIGAIKQT
IDQLQPEFVIIDSIQTMIHPDVDSAAGSVSQVRATTAELMQIAKTNHIAIFIVGHVTKEGSLAGPRMLEHMVDTVLYFEG
DRHHTFRILRAVKNRFGSTNEIGIFEMRQIGLVEVLNPSEAFLEERIDGATGSAIVCSMEGTRPILAEVQSLVTPSVFGN
AKRTATGLDYNRVSLIMAVLEKRAGLMLQNQDAYLKAAGGVKLDEPAIDLAIAVSIASSYKDQGTLPSDCFIGEIGLTGE
VRRVNRIDQRVLEAQKLGFKRAYVPKNNLPGWDKPQGIEVIGVETVSETLHKVFS

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=921971 VSF34_RS01410 WP_326717342.1 260492..261859(+) (radA) [Vagococcus jeotgali strain B2T-5]
ATGGCTAAAAAGAAAAATGAATATGTCTGTCAACAATGTGGGTATAAATCTCCTAAGTATCTTGGAAGGTGTCCTAATTG
CGGAGAGTGGAACACTTTAGTTGAGGAGATTATTCAAGATAAACCAACAAGACATTCTCGTGTCAATTTATCTGGTGAGG
GCAGCAAAGTTCAAAAATTAAAAGAGGTCACAAGTCAAAAAGAGCCTCGTGTGAAAACAAATCTAGAGGAATTAAACCGT
GTGCTAGGTGGTGGAGTAGTACCAGGTTCTATGGTCTTAATTGGTGGTGACCCAGGTATTGGTAAATCAACACTACTTTT
ACAAGTATCTCAGCAACTTAGCCTCTTAGATGGTAATGTGTTATATGTTTCTGGTGAGGAAAGCTCTGAACAAATAAAAA
TGCGAGCAGAGCGTCTGCAAGATGGTAGTAATGATTTTTATGTGTACCCAGAAACAGATATCGGTGCCATTAAGCAAACC
ATTGACCAACTACAACCAGAATTTGTTATCATTGATTCGATTCAAACAATGATTCATCCAGATGTAGATAGCGCAGCTGG
TAGTGTAAGTCAAGTCAGAGCTACAACGGCTGAATTGATGCAAATTGCTAAGACGAACCATATTGCGATTTTTATTGTGG
GACACGTCACAAAAGAGGGGTCTTTAGCTGGCCCTCGGATGCTTGAGCATATGGTGGATACTGTTCTTTATTTTGAAGGT
GACAGGCATCATACGTTTCGTATATTACGAGCCGTCAAAAATAGGTTTGGCTCTACCAATGAAATTGGGATTTTTGAAAT
GAGACAAATCGGACTGGTAGAAGTATTAAATCCATCCGAAGCTTTTTTAGAAGAGCGGATTGACGGGGCAACAGGTTCTG
CTATTGTGTGTTCTATGGAGGGGACAAGGCCGATTTTAGCTGAAGTCCAGTCCCTTGTAACACCAAGTGTATTTGGTAAT
GCTAAGCGTACTGCAACGGGTCTTGACTATAATAGGGTATCACTTATTATGGCTGTTTTAGAAAAAAGAGCTGGACTTAT
GCTTCAAAATCAAGATGCTTACCTTAAAGCTGCTGGAGGTGTTAAATTAGATGAACCAGCGATTGATTTAGCCATTGCCG
TCAGCATTGCCTCAAGTTATAAAGATCAAGGAACGCTACCATCAGATTGCTTTATTGGTGAAATTGGATTAACTGGTGAA
GTTAGACGAGTCAACCGGATTGATCAACGTGTGTTAGAAGCACAAAAATTAGGATTTAAGCGTGCCTATGTACCAAAAAA
TAATTTACCTGGTTGGGATAAACCACAAGGAATTGAAGTGATTGGGGTTGAAACAGTATCAGAAACTCTACACAAAGTAT
TTTCATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(70-220)

Subunit ChlI of Mg-chelatase (ChlI)

(362-428)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

72.088

100

0.721
  radA Streptococcus pneumoniae Rx1

72.088

100

0.721
  radA Streptococcus pneumoniae D39

72.088

100

0.721
  radA Streptococcus pneumoniae R6

72.088

100

0.721
  radA Streptococcus pneumoniae TIGR4

72.088

100

0.721
  radA Streptococcus mitis SK321

72.088

100

0.721
  radA Bacillus subtilis subsp. subtilis str. 168

67.181

99.78

0.67