Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   VP456_RS13330 Genome accession   NZ_CP142142
Coordinates   2664753..2665553 (-) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus velezensis strain PD9     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 2659753..2670553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VP456_RS13300 (VP456_13265) - 2660301..2660933 (+) 633 WP_014418348.1 helix-turn-helix domain-containing protein -
  VP456_RS13305 (VP456_13270) - 2661224..2661361 (+) 138 WP_014470646.1 hypothetical protein -
  VP456_RS13310 (VP456_13275) - 2661455..2662573 (+) 1119 WP_029973892.1 NADH-dependent flavin oxidoreductase -
  VP456_RS13315 (VP456_13280) - 2662699..2662911 (+) 213 WP_029973891.1 spore germination protein -
  VP456_RS13320 (VP456_13285) - 2663037..2663249 (+) 213 WP_014418351.1 spore germination protein -
  VP456_RS13325 (VP456_13290) alr 2663424..2664605 (+) 1182 WP_377864790.1 alanine racemase -
  VP456_RS13330 (VP456_13295) spo0A 2664753..2665553 (-) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  VP456_RS13335 (VP456_13300) spoIVB 2665834..2667114 (-) 1281 WP_029973889.1 SpoIVB peptidase -
  VP456_RS13340 (VP456_13305) recN 2667278..2669008 (-) 1731 WP_007408359.1 DNA repair protein RecN Machinery gene
  VP456_RS13345 (VP456_13310) ahrC 2669040..2669489 (-) 450 WP_003153173.1 transcriptional regulator AhrC/ArgR -
  VP456_RS13350 (VP456_13315) - 2669586..2670431 (-) 846 WP_029973888.1 TlyA family RNA methyltransferase -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=921924 VP456_RS13330 WP_003153177.1 2664753..2665553(-) (spo0A) [Bacillus velezensis strain PD9]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=921924 VP456_RS13330 WP_003153177.1 2664753..2665553(-) (spo0A) [Bacillus velezensis strain PD9]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCTGATGTACTCG
TTTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGGGAATCAGAGCTCGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGATGTTACGAAAAAAGCCGTCGATTTAGGCGCGTCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCGC
CGTCCGCGCAAAGCAGCATTATCCGCAGCCAGCCGGAGCCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACCGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAACCAACCAATTCAGAATTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97