Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   VPX56_RS20270 Genome accession   NZ_CP142124
Coordinates   4252109..4253542 (+) Length   477 a.a.
NCBI ID   WP_326468592.1    Uniprot ID   -
Organism   Enterobacter wuhouensis strain AV1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4247109..4258542
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VPX56_RS20240 (VPX56_20240) - 4247676..4248653 (+) 978 WP_131635303.1 calcium/sodium antiporter -
  VPX56_RS20245 (VPX56_20245) kdsD 4248667..4249653 (+) 987 WP_131635302.1 arabinose-5-phosphate isomerase KdsD -
  VPX56_RS20250 (VPX56_20250) kdsC 4249668..4250234 (+) 567 WP_131635301.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  VPX56_RS20255 (VPX56_20255) lptC 4250231..4250806 (+) 576 WP_131635300.1 LPS export ABC transporter periplasmic protein LptC -
  VPX56_RS20260 (VPX56_20260) lptA 4250775..4251329 (+) 555 WP_131635299.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  VPX56_RS20265 (VPX56_20265) lptB 4251336..4252061 (+) 726 WP_131635298.1 LPS export ABC transporter ATP-binding protein -
  VPX56_RS20270 (VPX56_20270) rpoN/rpoN1 4252109..4253542 (+) 1434 WP_326468592.1 RNA polymerase factor sigma-54 Machinery gene
  VPX56_RS20275 (VPX56_20275) hpf 4253565..4253852 (+) 288 WP_119936816.1 ribosome hibernation promoting factor -
  VPX56_RS20280 (VPX56_20280) ptsN 4253935..4254426 (+) 492 WP_131635296.1 PTS IIA-like nitrogen regulatory protein PtsN -
  VPX56_RS20285 (VPX56_20285) rapZ 4254472..4255326 (+) 855 WP_131635295.1 RNase adapter RapZ -
  VPX56_RS20290 (VPX56_20290) npr 4255323..4255595 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  VPX56_RS20295 (VPX56_20295) mtgA 4255635..4256360 (-) 726 WP_131635294.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  VPX56_RS20300 (VPX56_20300) elbB 4256357..4257010 (-) 654 WP_326472148.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54253.99 Da        Isoelectric Point: 4.3123

>NTDB_id=921878 VPX56_RS20270 WP_326468592.1 4252109..4253542(+) (rpoN/rpoN1) [Enterobacter wuhouensis strain AV1]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTETLDTVDALEQKEMPD
ELPLDASWDELYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESIGDDEIELEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLDEARLIISDHLDLLANHDFRTLMRVTR
QKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSVRNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVSTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=921878 VPX56_RS20270 WP_326468592.1 4252109..4253542(+) (rpoN/rpoN1) [Enterobacter wuhouensis strain AV1]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAACAACTGGCAATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
GTTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGATACTCAGCAATCGCAGGACACTGAAACGCTCGATACCGTTGATGCGCTCGAACAAAAAGAGATGCCGGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAACTCTACACCGCCGGAACGCCTTCGGGCACGCGTGCAGATTATCAGGACGA
CGAGCTGCCGGTTTATCAGGGCGAAACGACCCAGTCGCTGCAGGACTACCTGATGTGGCAAGTTGAGCTGACGCCCTTCT
CCGACACCGACCGCGCAATTGCGACGTCAATTGTCGATGCCGTCGATGACACCGGCTACTTGACCGTCACGCTTGACGAT
ATTCTGGAAAGCATCGGCGACGACGAGATTGAACTCGAAGAAATTGAAGCCGTCCTGAAGCGCATCCAGCGCTTCGATCC
GGTCGGGGTCGCGGCAAAAGATCTGCGCGATTGCCTGCTGATCCAGCTCTCGCAGTTCGACAAAACCACGCCCTGGCTTG
ATGAGGCCCGATTAATCATCAGCGATCATCTGGATCTGCTGGCTAACCACGATTTCCGTACCCTGATGCGCGTCACGCGC
CAGAAGGAAGAGGTGCTGAAAGAAGCGGTTAACCTGATCCAGTCGCTCGATCCGCGCCCCGGTCAGTCGATCCAGACCAG
CGAACCCGAGTATGTCATCCCGGATGTCCTGGTCAGAAAGCACAACGACCGCTGGGTCGTTGAACTGAACTCAGACAGCA
TTCCTCGCCTGCAAATTAATCAGCAATACGCCTCCATGTGCACCAGCGTGCGTAACGACTCAGACAATCAATATATTCGC
AGCAACCTGCAGGAAGCGCGATGGCTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTGCGCGTAAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTACATGAAGCCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGCCCGCGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAGCACCGAGGGCGGTGGCGAAGCCTCGTCCACGGCGATCCGTGCACTGGTGAAGAAGTT
GATCGCAGCGGAGAACCCCGCGAAGCCACTGAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48