Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   DGAOKPDK_RS13165 Genome accession   NZ_CP142019
Coordinates   2790518..2791318 (-) Length   266 a.a.
NCBI ID   WP_324641742.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain EMB-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2785518..2796318
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DGAOKPDK_RS13145 (VOI31_RS13140) - 2786583..2787281 (-) 699 WP_000058788.1 YfgM family protein -
  DGAOKPDK_RS13150 (VOI31_RS13145) hisS 2787310..2788602 (-) 1293 WP_000095254.1 histidine--tRNA ligase -
  DGAOKPDK_RS13155 (VOI31_RS13150) ispG 2788599..2789714 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  DGAOKPDK_RS13160 (VOI31_RS13155) - 2789736..2790527 (-) 792 WP_000405161.1 helix-turn-helix domain-containing protein -
  DGAOKPDK_RS13165 (VOI31_RS13160) pilF 2790518..2791318 (-) 801 WP_324641742.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  DGAOKPDK_RS13170 (VOI31_RS13165) rlmN 2791334..2792566 (-) 1233 WP_032043679.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  DGAOKPDK_RS13175 (VOI31_RS13170) ndk 2792689..2793120 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  DGAOKPDK_RS13180 (VOI31_RS13175) iscX 2793234..2793434 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  DGAOKPDK_RS13185 (VOI31_RS13180) - 2793534..2794085 (-) 552 WP_000669568.1 phosphatase PAP2 family protein -
  DGAOKPDK_RS13190 (VOI31_RS13185) - 2794110..2795408 (-) 1299 WP_001991191.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30108.47 Da        Isoelectric Point: 9.2307

>NTDB_id=921060 DGAOKPDK_RS13165 WP_324641742.1 2790518..2791318(-) (pilF) [Acinetobacter baumannii strain EMB-1]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYLRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=921060 DGAOKPDK_RS13165 WP_324641742.1 2790518..2791318(-) (pilF) [Acinetobacter baumannii strain EMB-1]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATCTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

73.846

97.744

0.722