Detailed information NTG0920831

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: VML59_RS23970
• Genome accession: NZ_CP142003
• Coordinates: 4579143..4580492 (-)
• Length: 449 a.a.
• NCBI ID: WP_088106316.1
• Uniprot ID: A0A1Y5Z255
• Organism: Bacillus pacificus strain AT31
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4574143..4585492

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VML59_RS23950	secA	4574468..4576975 (-)	2508	WP_000579366.1	preprotein translocase subunit SecA	-
VML59_RS23955	hpf	4577251..4577793 (-)	543	WP_000671190.1	ribosome hibernation-promoting factor, HPF/YfiA family	-
VML59_RS23960	cspC	4578115..4578312 (-)	198	WP_001162041.1	cold shock protein CspC	-
VML59_RS23965	-	4578439..4579143 (-)	705	WP_001998620.1	ComF family protein	-
VML59_RS23970	comFA	4579143..4580492 (-)	1350	WP_088106316.1	ATP-dependent helicase ComFA	Machinery gene
VML59_RS23975	-	4580619..4582064 (-)	1446	WP_041184798.1	NlpC/P60 family protein	-
VML59_RS23980	-	4582211..4582525 (-)	315	WP_000400857.1	winged helix-turn-helix transcriptional regulator	-
VML59_RS23985	-	4582699..4583541 (-)	843	WP_000844764.1	DegV family protein	-
VML59_RS23990	-	4583781..4584416 (+)	636	WP_000926694.1	YigZ family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51083.73 Da
• Isoelectric Point: 9.9947

>NTDB_id=920831 VML59_RS23970 WP_088106316.1 4579143..4580492(-) (comFA) [Bacillus pacificus strain AT31]
MLAGKQLLLEELSSDLRRELSDLKKKGEVVCVQGITKKASKYICQRCGNIEQRLFASFLCKRCGKACAYCRKCITMGRVS
ECTVLVRGIHERNGDRELNPLQWNGTLSIGQELAAQGVKEAIKQKKSFFIWAVCGAGKTEMLFYGIEEALRKGERVCIAT
PRTDVVLELAPRLQEVFPSITVAALYGGSVDHKKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVRQAM
KEKAARIYLTATPDEKWKRNFRKGKQKGVIVSGRYHRHPLPVPLFSWCGNWKKSLHHKKIPRVLLQWLKMNLNKKYPIFL
FVPHVRYIEEISQLLKGLDNRIDGVHAEDSMRKEKVASFRKGDIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEEPYGEVMYFHYGKTEAMVRAKKHIQSMNKNAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=920831 VML59_RS23970 WP_088106316.1 4579143..4580492(-) (comFA) [Bacillus pacificus strain AT31]
ATGCTTGCTGGAAAACAGTTGCTATTAGAAGAACTCTCTTCAGATTTACGGAGAGAATTAAGTGATTTGAAAAAGAAGGG
AGAGGTTGTATGTGTACAAGGTATAACGAAGAAGGCTTCTAAATATATATGTCAGCGCTGCGGAAATATAGAGCAGCGGC
TATTTGCATCATTTTTATGTAAAAGGTGCGGTAAAGCATGCGCGTATTGCCGGAAGTGTATCACGATGGGGAGAGTTAGT
GAATGTACTGTACTTGTTCGCGGGATTCATGAAAGAAACGGGGACAGGGAGCTAAATCCATTACAGTGGAATGGGACTTT
ATCGATTGGTCAGGAGTTGGCGGCGCAAGGGGTTAAAGAAGCGATTAAGCAGAAAAAATCTTTTTTTATTTGGGCTGTGT
GCGGGGCTGGAAAAACAGAAATGTTATTTTACGGTATAGAAGAGGCGCTTCGAAAAGGAGAAAGAGTTTGTATCGCAACG
CCAAGGACGGACGTTGTACTGGAATTAGCGCCGAGATTACAAGAAGTGTTTCCAAGTATAACTGTAGCTGCTTTATACGG
AGGGAGTGTAGATCATAAAAAAGATGCAGCGTTAGTCGTTGCAACGACTCACCAACTGTTACGTTACTATAGAGCGTTTC
ATGTGATGATTGTAGATGAGATTGATGCCTTCCCGTATCATGCAGATCAAATGTTACAGTATGCAGTTAGGCAAGCAATG
AAAGAGAAAGCAGCGCGTATTTATTTAACTGCAACCCCTGATGAGAAGTGGAAGCGTAATTTTAGAAAGGGGAAACAAAA
AGGTGTTATTGTGTCAGGACGATACCATCGTCACCCGTTACCAGTTCCTCTATTTAGTTGGTGCGGAAATTGGAAGAAAA
GCCTCCATCATAAAAAAATTCCTCGCGTGTTACTACAATGGTTAAAAATGAACTTAAACAAAAAGTATCCTATTTTTTTA
TTTGTTCCTCATGTGCGATATATAGAAGAAATAAGTCAGTTGTTGAAAGGGTTGGATAATAGAATAGATGGCGTGCATGC
AGAAGATTCGATGAGAAAAGAAAAAGTAGCATCTTTCAGAAAGGGAGACATTCCGTTATTAGTTACAACGACAATTTTAG
AAAGAGGAGTAACTGTGAAGAACTTACAAGTGGCTGTGTTAGGAGCTGAAGAAGAAATTTTTTCAGAGAGTGCGCTCGTA
CAAATTGCAGGCCGAGCGGGGCGTAGTTTTGAAGAGCCATATGGTGAGGTTATGTATTTTCATTACGGTAAGACAGAGGC
AATGGTACGCGCGAAAAAACATATTCAAAGTATGAACAAAAATGCGAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (110-254)

• Helicase conserved C-terminal domain (Helicase_C) (311-408)

3D structure

• AlphaFold DB: A0A1Y5Z255

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	50.679	98.441	0.499
comFA	Latilactobacillus sakei subsp. sakei 23K	40	90.2	0.361