Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   VML59_RS19305 Genome accession   NZ_CP142003
Coordinates   3715243..3716286 (-) Length   347 a.a.
NCBI ID   WP_001013195.1    Uniprot ID   A0AAP5FWA8
Organism   Bacillus pacificus strain AT31     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3710243..3721286
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VML59_RS19260 - 3710671..3710871 (-) 201 WP_000106079.1 YqzE family protein -
  VML59_RS19265 aroK 3710910..3711407 (-) 498 WP_000836625.1 shikimate kinase AroK -
  VML59_RS19270 - 3711527..3712177 (-) 651 WP_000183849.1 2OG-Fe(II) oxygenase -
  VML59_RS19275 comGG 3712353..3712724 (-) 372 WP_074567439.1 competence type IV pilus minor pilin ComGG -
  VML59_RS19280 comGF 3712721..3713191 (-) 471 WP_000923092.1 competence type IV pilus minor pilin ComGF -
  VML59_RS19285 comGE 3713161..3713463 (-) 303 WP_124878201.1 competence type IV pilus minor pilin ComGE -
  VML59_RS19290 comGD 3713456..3713911 (-) 456 WP_000810388.1 comG operon protein ComGD -
  VML59_RS19295 comGC 3713908..3714207 (-) 300 WP_001178694.1 competence type IV pilus major pilin ComGC -
  VML59_RS19300 comGB 3714219..3715250 (-) 1032 WP_074567440.1 competence type IV pilus assembly protein ComGB -
  VML59_RS19305 comGA 3715243..3716286 (-) 1044 WP_001013195.1 competence protein ComGA Machinery gene
  VML59_RS19310 - 3716492..3717187 (+) 696 WP_014893514.1 helix-turn-helix transcriptional regulator -
  VML59_RS19315 - 3717313..3717555 (+) 243 WP_000440713.1 DUF2626 domain-containing protein -
  VML59_RS19320 - 3717663..3719057 (+) 1395 WP_001094339.1 L-cystine transporter -
  VML59_RS19325 - 3719137..3719529 (-) 393 WP_049113842.1 hypothetical protein -
  VML59_RS19330 - 3719617..3719832 (-) 216 WP_001008330.1 DUF3912 family protein -
  VML59_RS19335 - 3720085..3720396 (+) 312 WP_014893513.1 hypothetical protein -
  VML59_RS19340 - 3720432..3720920 (-) 489 WP_000764258.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39359.83 Da        Isoelectric Point: 9.0210

>NTDB_id=920820 VML59_RS19305 WP_001013195.1 3715243..3716286(-) (comGA) [Bacillus pacificus strain AT31]
MNGIESFANMILKEACRVQASDLHIVPRQKDVVVQLRIGKDLMTKQYIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
IQIDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKKLLSFLHYSHGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIVTLEDPVEKRNDDVLQIQINEKAGITYEAGLKAILRHDPDVILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAILRFMDFGITRQEIEQSLLAIAAQRLVELKCPFCKRKCSTVCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYKHETLESSLRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=920820 VML59_RS19305 WP_001013195.1 3715243..3716286(-) (comGA) [Bacillus pacificus strain AT31]
ATGAATGGAATTGAAAGCTTTGCGAATATGATTTTAAAAGAAGCGTGCAGGGTACAAGCGTCGGACTTACATATTGTGCC
CCGGCAGAAGGATGTAGTGGTTCAACTGCGCATAGGAAAAGATTTAATGACGAAACAATACATTGAAAAGGAATTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCCTCCATGGATATAGGAGAGAGGCGGAAACCACAAAACGGTTCACTATAT
ATACAAATAGATGGACAAGAAGTGTATTTACGCCTTTCCACGCTTCCAACCGTATATCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCGTCTATTCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACAGCGAAGAAACTACTTTCTTTTTTAC
ATTATTCCCATGGATTACTCGTCTTTACGGGACCGACTGGTTCAGGAAAGACAACAACAATGTATGCATTATTAGAGGTG
ATTAGAAAAAAGAAAACACGTCGCATCGTTACGCTGGAAGATCCAGTTGAAAAAAGAAATGACGATGTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCTGGACTAAAGGCTATTTTGCGTCATGATCCAGATGTTATTTTAGTGG
GTGAAATTCGTGATGAAGAAACAGCAAAAATCGCTATAAGAGCAAGTTTGACTGGCCATTTAGTAATGACGACACTGCAT
ACGAATGATGCGAAAGGAGCAATACTTCGGTTCATGGATTTTGGTATAACGAGGCAAGAGATTGAACAATCTTTATTGGC
TATAGCTGCACAGCGACTTGTTGAATTGAAGTGTCCGTTTTGCAAAAGAAAGTGCTCAACTGTATGTAAATCAATGAGGC
AAGTAAGGCAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGAAGCAAACGGGGAATGT
GTCACGTACAAGCATGAGACATTAGAGTCTTCGCTGCGAAAAGGATACGCTTTAGGATTTTTAGAAGAGGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.925

100

0.579
  pilB Vibrio campbellii strain DS40M4

35.977

100

0.366