Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   VML59_RS18075 Genome accession   NZ_CP142003
Coordinates   3507915..3508349 (-) Length   144 a.a.
NCBI ID   WP_049111162.1    Uniprot ID   -
Organism   Bacillus pacificus strain AT31     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3502915..3513349
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VML59_RS18060 - 3503939..3504496 (+) 558 WP_000062077.1 PadR family transcriptional regulator -
  VML59_RS18065 metE 3505164..3507452 (+) 2289 WP_001007593.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  VML59_RS18070 comJ 3507490..3507879 (-) 390 WP_000424014.1 competence protein ComJ -
  VML59_RS18075 nucA/comI 3507915..3508349 (-) 435 WP_049111162.1 DNA-entry nuclease Machinery gene
  VML59_RS18080 - 3508739..3509488 (+) 750 WP_000388461.1 DUF3967 domain-containing protein -
  VML59_RS18085 kinB 3509542..3510816 (-) 1275 WP_000420388.1 sporulation sensor histidine kinase KinB -
  VML59_RS18090 - 3511126..3511326 (+) 201 WP_000929285.1 hypothetical protein -
  VML59_RS18095 - 3511450..3512613 (+) 1164 WP_049113003.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16028.41 Da        Isoelectric Point: 8.4331

>NTDB_id=920817 VML59_RS18075 WP_049111162.1 3507915..3508349(-) (nucA/comI) [Bacillus pacificus strain AT31]
MKQLKGIIISIIAILSIVVAMYEVLVPEETSIKKTNAYDQVLEFPKDRYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=920817 VML59_RS18075 WP_049111162.1 3507915..3508349(-) (nucA/comI) [Bacillus pacificus strain AT31]
ATGAAACAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGATGTATGAAGTGCTTGTTCC
AGAGGAAACAAGTATTAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGATCGATATCCAGAGACAGGGA
AACATATTACGGATGCTATAAAAGAAGGACATTCAGAAGTTTGTACAATCGACCGTGGTGGTGCTGCGGATAGAAGAAAA
TTATCGTTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCGCATATTGAATATATAAGTCCAGCCGATAACCGCGGAGCAGGCTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

72.072

77.083

0.556