Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   VIW49_RS20485 Genome accession   NZ_CP142002
Coordinates   4275263..4276696 (+) Length   477 a.a.
NCBI ID   WP_028014571.1    Uniprot ID   A0AAU7N964
Organism   Enterobacter bugandensis strain H4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4270263..4281696
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VIW49_RS20455 (VIW49_20455) - 4270830..4271807 (+) 978 WP_028014566.1 calcium/sodium antiporter -
  VIW49_RS20460 (VIW49_20460) kdsD 4271821..4272807 (+) 987 WP_326431382.1 arabinose-5-phosphate isomerase KdsD -
  VIW49_RS20465 (VIW49_20465) kdsC 4272822..4273388 (+) 567 WP_025756695.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  VIW49_RS20470 (VIW49_20470) lptC 4273385..4273960 (+) 576 WP_028014568.1 LPS export ABC transporter periplasmic protein LptC -
  VIW49_RS20475 (VIW49_20475) lptA 4273929..4274483 (+) 555 WP_028014569.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  VIW49_RS20480 (VIW49_20480) lptB 4274490..4275215 (+) 726 WP_028014570.1 LPS export ABC transporter ATP-binding protein -
  VIW49_RS20485 (VIW49_20485) rpoN/rpoN1 4275263..4276696 (+) 1434 WP_028014571.1 RNA polymerase factor sigma-54 Machinery gene
  VIW49_RS20490 (VIW49_20490) hpf 4276719..4277006 (+) 288 WP_014171818.1 ribosome hibernation promoting factor -
  VIW49_RS20495 (VIW49_20495) ptsN 4277089..4277580 (+) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  VIW49_RS20500 (VIW49_20500) rapZ 4277626..4278480 (+) 855 WP_014885397.1 RNase adapter RapZ -
  VIW49_RS20505 (VIW49_20505) npr 4278477..4278749 (+) 273 WP_025756690.1 PTS phosphocarrier protein NPr -
  VIW49_RS20510 (VIW49_20510) mtgA 4278788..4279513 (-) 726 WP_063438544.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  VIW49_RS20515 (VIW49_20515) elbB 4279510..4280163 (-) 654 WP_326431387.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54181.95 Da        Isoelectric Point: 4.3173

>NTDB_id=920781 VIW49_RS20485 WP_028014571.1 4275263..4276696(+) (rpoN/rpoN1) [Enterobacter bugandensis strain H4]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQADLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDEEIEIEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=920781 VIW49_RS20485 WP_028014571.1 4275263..4276696(+) (rpoN/rpoN1) [Enterobacter bugandensis strain H4]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGCTGGCGATGACGCCGCAGTTACAGCAGGCAATTCGTCTGTTGCA
ACTGTCCACCTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCCCTGCTGGAGCAAGCCGATCTTCATG
ACGAGGTAGATACCCAGCAGTCACAGGACACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCGGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAAATCTATACCGCCGGGACCCCTTCCGGCACGCGTGCAGACTACCAGGACGA
CGAGCTACCGGTCTACCAGGGCGAAACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTAGAGCTTACCCCCTTCT
CCGATACCGACCGCGCTATTGCCACGTCGATTGTCGACGCCGTTGATGACACCGGTTATTTGACCGTCACGCTGGACGAC
ATTCTGGAAAGCATGGGCGATGAAGAGATTGAGATTGAGGAGATTGAAGCGGTACTGAAACGCATTCAGCGCTTCGATCC
GGTCGGGGTGGCCGCGAAGGACCTGCGTGACTGTCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCGTGGCTCG
ACGAGGCACGCTTAATCATCAGCGATCATCTGGATCTTCTGGCTAACCACGATTTCCGCACCCTGATGCGCGTGACGCGC
CTGAAGGAAGAGGTGCTGAAAGAGGCGGTAAATCTGATCCAGTCCCTCGATCCGCGTCCCGGTCAGTCGATCCAGACCAG
CGAACCCGAGTACGTTATCCCGGACGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCTGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTACGCCTCCATGTGTACCAGCGCGCGCAACGACTCCGACAATCAATATATTCGC
AGCAATCTTCAGGAAGCGCGATGGTTGATCAAGAGCCTGGAGAGCCGGAATGATACCCTGCTTCGCGTGAGCCGCTGCAT
CGTCGAACAGCAGCAGGCCTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCCACTATTTCCCGCGTTACTACGCAGAAGTATCTGCACAGTCCGCGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAGGGTGGCGGCGAAGCCTCCTCAACGGCGATTCGCGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAATCCCGCGAAGCCACTAAGTGACAGTAAGTTGACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48