Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   VDS58_RS14335 Genome accession   NZ_CP141997
Coordinates   2626244..2626654 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain PM0031     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2621244..2631654
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VDS58_RS14305 (VDS58_14305) - 2621330..2621470 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  VDS58_RS14310 (VDS58_14310) yqeF 2621776..2622507 (-) 732 WP_260665349.1 SGNH/GDSL hydrolase family protein -
  VDS58_RS14315 (VDS58_14315) cwlH 2622759..2623511 (-) 753 WP_070547555.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  VDS58_RS14320 (VDS58_14320) yqeD 2623698..2624324 (+) 627 WP_260665348.1 TVP38/TMEM64 family protein -
  VDS58_RS14325 (VDS58_14325) gnd 2624344..2625237 (-) 894 WP_260665347.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  VDS58_RS14330 (VDS58_14330) yqeB 2625489..2626211 (+) 723 WP_032726205.1 hypothetical protein -
  VDS58_RS14335 (VDS58_14335) nucA/comI 2626244..2626654 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  VDS58_RS14340 (VDS58_14340) sigK 2626850..2627578 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  VDS58_RS14345 (VDS58_14345) - 2627578..2627694 (+) 117 WP_041850087.1 hypothetical protein -
  VDS58_RS14350 (VDS58_14350) - 2627660..2627857 (-) 198 Protein_2782 recombinase family protein -
  VDS58_RS14355 (VDS58_14355) - 2627815..2627993 (-) 179 Protein_2783 hypothetical protein -
  VDS58_RS14360 (VDS58_14360) psiE 2628534..2628950 (-) 417 WP_003246154.1 phosphate-starvation-inducible protein PsiE -
  VDS58_RS14365 (VDS58_14365) yrkQ 2628996..2630294 (-) 1299 WP_049141120.1 two-component system sensor histidine kinase YrkQ -
  VDS58_RS14370 (VDS58_14370) yrkP 2630281..2630976 (-) 696 WP_021480140.1 two-component response regulator YrkP -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=920738 VDS58_RS14335 WP_009967785.1 2626244..2626654(-) (nucA/comI) [Bacillus subtilis strain PM0031]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=920738 VDS58_RS14335 WP_009967785.1 2626244..2626654(-) (nucA/comI) [Bacillus subtilis strain PM0031]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCAATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529