Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AB3N51_RS15795 Genome accession   NZ_CP162190
Coordinates   3248700..3250133 (+) Length   477 a.a.
NCBI ID   WP_000809036.1    Uniprot ID   -
Organism   Escherichia coli strain STK77-E     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3243700..3255133
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3N51_RS15765 (AB3N51_15765) yrbG 3244258..3245235 (+) 978 WP_000922901.1 calcium/sodium antiporter -
  AB3N51_RS15770 (AB3N51_15770) kdsD 3245249..3246235 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  AB3N51_RS15775 (AB3N51_15775) kdsC 3246256..3246822 (+) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  AB3N51_RS15780 (AB3N51_15780) lptC 3246819..3247394 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  AB3N51_RS15785 (AB3N51_15785) lptA 3247363..3247920 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  AB3N51_RS15790 (AB3N51_15790) lptB 3247927..3248652 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  AB3N51_RS15795 (AB3N51_15795) rpoN/rpoN1 3248700..3250133 (+) 1434 WP_000809036.1 RNA polymerase factor sigma-54 Machinery gene
  AB3N51_RS15800 (AB3N51_15800) hpf 3250156..3250443 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  AB3N51_RS15805 (AB3N51_15805) ptsN 3250561..3251052 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AB3N51_RS15810 (AB3N51_15810) rapZ 3251098..3251952 (+) 855 WP_000243741.1 RNase adapter RapZ -
  AB3N51_RS15815 (AB3N51_15815) npr 3251949..3252221 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  AB3N51_RS15820 (AB3N51_15820) yrbL 3252435..3253067 (+) 633 WP_000620387.1 PhoP regulatory network protein YrbL -
  AB3N51_RS15825 (AB3N51_15825) mtgA 3253064..3253792 (-) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  AB3N51_RS15830 (AB3N51_15830) elbB 3253789..3254442 (-) 654 WP_001299134.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54007.82 Da        Isoelectric Point: 4.3639

>NTDB_id=920587 AB3N51_RS15795 WP_000809036.1 3248700..3250133(+) (rpoN/rpoN1) [Escherichia coli strain STK77-E]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPIYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=920587 AB3N51_RS15795 WP_000809036.1 3248700..3250133(+) (rpoN/rpoN1) [Escherichia coli strain STK77-E]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGATCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGATTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACAGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCTCGCAATGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465