Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   VK758_RS17365 Genome accession   NZ_CP141897
Coordinates   3608369..3609802 (-) Length   477 a.a.
NCBI ID   WP_028014571.1    Uniprot ID   A0AAU7N964
Organism   Enterobacter bugandensis strain Eltt.P1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3603369..3614802
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VK758_RS17335 (VK758_17335) elbB 3604902..3605555 (+) 654 WP_248111016.1 isoprenoid biosynthesis glyoxalase ElbB -
  VK758_RS17340 (VK758_17340) mtgA 3605552..3606277 (+) 726 WP_324660651.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  VK758_RS17345 (VK758_17345) npr 3606316..3606588 (-) 273 WP_025756690.1 PTS phosphocarrier protein NPr -
  VK758_RS17350 (VK758_17350) rapZ 3606585..3607439 (-) 855 WP_014885397.1 RNase adapter RapZ -
  VK758_RS17355 (VK758_17355) ptsN 3607485..3607976 (-) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  VK758_RS17360 (VK758_17360) hpf 3608059..3608346 (-) 288 WP_014171818.1 ribosome hibernation promoting factor -
  VK758_RS17365 (VK758_17365) rpoN/rpoN1 3608369..3609802 (-) 1434 WP_028014571.1 RNA polymerase factor sigma-54 Machinery gene
  VK758_RS17370 (VK758_17370) lptB 3609850..3610575 (-) 726 WP_028014570.1 LPS export ABC transporter ATP-binding protein -
  VK758_RS17375 (VK758_17375) lptA 3610582..3611136 (-) 555 WP_028014569.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  VK758_RS17380 (VK758_17380) lptC 3611105..3611680 (-) 576 WP_028014568.1 LPS export ABC transporter periplasmic protein LptC -
  VK758_RS17385 (VK758_17385) kdsC 3611677..3612243 (-) 567 WP_025756695.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  VK758_RS17390 (VK758_17390) kdsD 3612258..3613244 (-) 987 WP_028014567.1 arabinose-5-phosphate isomerase KdsD -
  VK758_RS17395 (VK758_17395) - 3613258..3614235 (-) 978 WP_028014566.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54181.95 Da        Isoelectric Point: 4.3173

>NTDB_id=920416 VK758_RS17365 WP_028014571.1 3608369..3609802(-) (rpoN/rpoN1) [Enterobacter bugandensis strain Eltt.P1]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQADLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDEEIEIEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=920416 VK758_RS17365 WP_028014571.1 3608369..3609802(-) (rpoN/rpoN1) [Enterobacter bugandensis strain Eltt.P1]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGCTGGCGATGACGCCGCAGTTACAGCAGGCAATTCGTCTGTTGCA
ACTGTCCACCTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCCCTGCTGGAGCAAGCCGATCTTCATG
ACGAGGTAGATACCCAGCAGTCACAGGACACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCGGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAAATCTATACCGCCGGGACCCCTTCCGGCACGCGTGCAGACTACCAGGACGA
CGAGCTACCGGTCTACCAGGGCGAAACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTAGAGCTTACCCCCTTCT
CCGATACCGACCGCGCTATTGCCACGTCGATTGTCGACGCCGTTGATGACACCGGTTATTTGACCGTCACGCTGGACGAC
ATTCTGGAAAGCATGGGCGATGAAGAGATTGAGATTGAGGAGATTGAAGCGGTACTGAAACGCATTCAGCGCTTCGATCC
GGTCGGGGTGGCCGCGAAGGACCTGCGTGACTGTCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCGTGGCTCG
ACGAGGCACGCTTAATCATCAGCGATCATCTGGATCTTCTGGCTAACCACGATTTCCGCACCCTGATGCGCGTGACGCGC
CTGAAGGAAGAGGTGCTGAAAGAGGCGGTAAATCTGATCCAGTCCCTCGATCCGCGTCCCGGTCAGTCGATCCAGACCAG
CGAACCCGAGTACGTTATCCCGGACGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCTGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTACGCCTCCATGTGTACCAGCGCGCGCAACGACTCCGACAATCAATATATTCGC
AGCAATCTTCAGGAAGCGCGATGGTTGATCAAGAGCCTGGAGAGCCGGAATGATACCCTGCTTCGCGTGAGCCGCTGCAT
CGTCGAACAGCAGCAGGCCTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCCACTATTTCCCGCGTTACTACGCAGAAGTATCTGCACAGTCCGCGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAGGGTGGCGGCGAAGCCTCCTCAACGGCGATTCGCGCCTTGGTGAAGAAGTT
GATCGCCGCGGAGAATCCCGCGAAGCCACTAAGTGACAGTAAGTTGACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48