Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   VK677_RS20725 Genome accession   NZ_CP141894
Coordinates   4492680..4494119 (-) Length   479 a.a.
NCBI ID   WP_017784940.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain 202108C2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4487680..4499119
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VK677_RS20695 (VK677_20695) dagF 4487955..4488695 (+) 741 WP_201889598.1 2-dehydro-3-deoxy-phosphogluconate aldolase -
  VK677_RS20700 (VK677_20700) - 4488800..4490704 (+) 1905 WP_201889597.1 BglG family transcription antiterminator -
  VK677_RS20705 (VK677_20705) npr 4490720..4490992 (-) 273 WP_005305943.1 PTS phosphocarrier protein NPr -
  VK677_RS20710 (VK677_20710) rapZ 4491013..4491879 (-) 867 WP_005305946.1 RNase adapter RapZ -
  VK677_RS20715 (VK677_20715) ptsN 4491922..4492368 (-) 447 WP_005305951.1 PTS IIA-like nitrogen regulatory protein PtsN -
  VK677_RS20720 (VK677_20720) hpf 4492371..4492658 (-) 288 WP_005305957.1 ribosome hibernation promoting factor -
  VK677_RS20725 (VK677_20725) rpoN/rpoN1 4492680..4494119 (-) 1440 WP_017784940.1 RNA polymerase factor sigma-54 Machinery gene
  VK677_RS20730 (VK677_20730) lptB 4494185..4494910 (-) 726 WP_005305964.1 LPS export ABC transporter ATP-binding protein -
  VK677_RS20735 (VK677_20735) lptA 4494914..4495441 (-) 528 WP_099359776.1 lipopolysaccharide transport periplasmic protein LptA -
  VK677_RS20740 (VK677_20740) lptC 4495428..4495988 (-) 561 WP_010635927.1 LPS export ABC transporter periplasmic protein LptC -
  VK677_RS20745 (VK677_20745) kdsC 4495985..4496539 (-) 555 WP_201889596.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  VK677_RS20750 (VK677_20750) - 4496539..4497534 (-) 996 WP_139397670.1 KpsF/GutQ family sugar-phosphate isomerase -
  VK677_RS20755 (VK677_20755) mlaF 4497742..4498542 (+) 801 WP_005305979.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53876.83 Da        Isoelectric Point: 4.3584

>NTDB_id=920321 VK677_RS20725 WP_017784940.1 4492680..4494119(-) (rpoN/rpoN1) [Aeromonas dhakensis strain 202108C2]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENDVQETSPVDQVDVSQLDSSDAMAQDQMP
DELPVDTTWEEVYSAGTAQSAGPIHDGEDSIYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVSLED
IQAAVSSEDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLNEAREVILEHMDLLGNRDYRTLARK
TKLRENDLKEVLDLIQQLEPRPGNGVIQSESQYVIPDVSVVKKGNKWVVELNPDSAPRIRVNETYAAMARQARTSQDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=920321 VK677_RS20725 WP_017784940.1 4492680..4494119(-) (rpoN/rpoN1) [Aeromonas dhakensis strain 202108C2]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGACAACAACCCCTTGCTGGAGCAAGAGGAAAACGACG
TTCAGGAGACCAGCCCGGTCGACCAGGTGGACGTCAGCCAGCTCGATTCCAGCGATGCCATGGCCCAGGACCAGATGCCG
GACGAACTGCCGGTGGATACCACCTGGGAAGAGGTCTACTCGGCCGGCACGGCCCAGAGCGCAGGCCCCATCCATGACGG
GGAAGACAGCATCTATCAGGGTGAGACCACCGAGAACCTGCAGGACTACCTGTTGTGGCAGATGCGCTTGACCCCGTTCA
GCGACGTGGATGCCGCCATCGCCCTCGCCATCATAGATGCTATCGACGAATCCGGTTATCTGACGGTGTCGCTTGAGGAT
ATTCAGGCGGCGGTGAGCAGCGAGGATGTCGAGGTGGAACTCGATGAAGTGGAGGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCCCGCTCGGTGCAGGAGTGCCTGCTGATCCAGCTGGGCCAGTACTCCCCTGACTTGCCCT
GGCTCAACGAAGCCAGGGAGGTGATCCTCGAGCACATGGATCTGCTCGGCAACCGCGATTACCGCACCCTGGCCCGCAAG
ACCAAGCTGAGAGAGAACGACCTCAAGGAGGTGCTGGATCTCATCCAGCAGCTGGAGCCGCGGCCCGGCAACGGCGTCAT
CCAGAGCGAATCCCAGTACGTGATCCCGGATGTTTCCGTGGTCAAGAAGGGCAACAAGTGGGTGGTCGAGCTCAACCCTG
ACTCGGCCCCGCGCATCCGGGTCAACGAAACCTATGCCGCCATGGCCAGACAGGCCCGCACCAGTCAGGATACCCAATTC
ATCCGCTCCCACCTGCAGGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGCCGCAACGACACCCTGCTCAAGGTGGCCAG
CTGCATCGTCGAGCAGCAGCAGGGCTTCTTCGAATACGGTGAAGAGGCCATGAAACCCATGGTGCTCAACGACATCGCCG
AAGCGGTGGAGATGCACGAGTCGACCATCTCGCGGGTGACCACCCAGAAGTACATGCACACGCCGCGCGGCATCTTCGAA
CTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAGGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCGGAGAATCCCGCCAAACCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.866

100

0.486