Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   VDS57_RS01070 Genome accession   NZ_CP141839
Coordinates   188660..189070 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain PN1236     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 183660..194070
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VDS57_RS01040 (VDS57_01040) - 183758..183898 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  VDS57_RS01045 (VDS57_01045) yqeF 184204..184935 (-) 732 WP_029317882.1 SGNH/GDSL hydrolase family protein -
  VDS57_RS01050 (VDS57_01050) cwlH 185187..185939 (-) 753 WP_017696287.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  VDS57_RS01055 (VDS57_01055) yqeD 186126..186752 (+) 627 WP_014480319.1 TVP38/TMEM64 family protein -
  VDS57_RS01060 (VDS57_01060) gnd 186771..187664 (-) 894 WP_324271581.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  VDS57_RS01065 (VDS57_01065) yqeB 187905..188627 (+) 723 WP_014480321.1 hypothetical protein -
  VDS57_RS01070 (VDS57_01070) nucA/comI 188660..189070 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  VDS57_RS01075 (VDS57_01075) sigK 189266..189994 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  VDS57_RS01080 (VDS57_01080) - 189994..190092 (+) 99 WP_031600702.1 hypothetical protein -
  VDS57_RS01085 (VDS57_01085) - 190089..190301 (-) 213 Protein_214 recombinase family protein -
  VDS57_RS01090 (VDS57_01090) fumC 190520..191908 (-) 1389 WP_103330414.1 class II fumarate hydratase -
  VDS57_RS01095 (VDS57_01095) - 192075..192965 (+) 891 WP_014480326.1 LysR family transcriptional regulator -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=920122 VDS57_RS01070 WP_009967785.1 188660..189070(-) (nucA/comI) [Bacillus subtilis strain PN1236]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=920122 VDS57_RS01070 WP_009967785.1 188660..189070(-) (nucA/comI) [Bacillus subtilis strain PN1236]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529